Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Dec;34(3):888–894. doi: 10.1128/iai.34.3.888-894.1981

Protection against feline leukemia by vaccination with a subunit vaccine.

M G Lewis, L E Mathes, R G Olsen
PMCID: PMC350952  PMID: 6277792

Abstract

An effective vaccine against feline leukemia virus infection has been developed by the collection and concentration of tissue culture medium harvested from a tumor cell line. Lymphoid cells were grown to near saturation density in a normal growth medium and then transferred to a serum-free medium. The serum-free medium was collected, concentrated, and evaluated for its vaccine potential. Cats receiving the vaccine emulsified in complete Freund adjuvant developed high antiviral and antitumor titers and were protected (81%) against virus challenge. Cats receiving the vaccine without an adjuvant developed lower antibody levels and lower protection (53%) from viremia. Age-matched and litter-matched controls developed no antibody to test antigens before the challenge, and 100% became persistently viremic after the challenge. Vaccination with the soluble tumor cell antigen vaccine proved successful in preventing the induction of feline leukemia virus infection.

Full text

PDF
888

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. J., Jarrett W. F., Jarrett O., Laird H. M. Feline leukemia-virus infection of kittens: mortality associated with atrophy of the thymus and lymphoid depletion. J Natl Cancer Inst. 1971 Oct;47(4):807–817. [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Cianciolo G. J., Matthews T. J., Bolognesi D. P., Snyderman R. Macrophage accumulation in mice is inhibited by low molecular weight products from murine leukemia viruses. J Immunol. 1980 Jun;124(6):2900–2905. [PubMed] [Google Scholar]
  4. Essex M., Klein G., Snyder S. P., Harrold J. B. Antibody to feline oncornavirus-associated cell membrane antigen in neonatal cats. Int J Cancer. 1971 Nov 15;8(3):384–390. doi: 10.1002/ijc.2910080305. [DOI] [PubMed] [Google Scholar]
  5. Hardy W. D., Jr, Hess P. W., MacEwen E. G., McClelland A. J., Zuckerman E. E., Essex M., Cotter S. M., Jarrett O. Biology of feline leukemia virus in the natural environment. Cancer Res. 1976 Feb;36(2 Pt 2):582–588. [PubMed] [Google Scholar]
  6. Hardy W. D., Jr, Hirshaut Y., Hess P. Detection of the feline leukemia virus and other mammalian oncornaviruses by immunofluorescence. Bibl Haematol. 1973;39:778–799. doi: 10.1159/000427906. [DOI] [PubMed] [Google Scholar]
  7. Hoover E. A., Mathes L. E., Rojko J. L., Schaller J. P., Olsen R. G. Modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. Am J Vet Res. 1978 Dec;39(12):1877–1880. [PubMed] [Google Scholar]
  8. JARRETT W. F., CRAWFORD E. M., MARTIN W. B., DAVIE F. A VIRUS-LIKE PARTICLE ASSOCIATED WITH LEUKEMIA (LYMPHOSARCOMA). Nature. 1964 May 9;202:567–569. doi: 10.1038/202567a0. [DOI] [PubMed] [Google Scholar]
  9. Jarrett W., Jarrett O., Mackey L., Laird H., Hood C., Hay D. Vaccination against feline leukaemia virus using a cell membrane antigen system. Int J Cancer. 1975 Jul 15;16(1):134–141. doi: 10.1002/ijc.2910160115. [DOI] [PubMed] [Google Scholar]
  10. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  11. Mathes L. E., Olsen R. G., Hebebrand L. C., Hoover E. A., Schaller J. P. Abrogation of lymphocyte blastogenesis by a feline leukaemia virus protein. Nature. 1978 Aug 17;274(5672):687–689. doi: 10.1038/274687a0. [DOI] [PubMed] [Google Scholar]
  12. Mathes L. E., Olsen R. G., Hebebrand L. C., Hoover E. A., Schaller J. P., Adams P. W., Nichols W. S. Immunosuppressive properties of a virion polypeptide, a 15,000-dalton protein, from feline leukemia virus. Cancer Res. 1979 Mar;39(3):950–955. [PubMed] [Google Scholar]
  13. Olsen R. G., Hoover E. A., Mathes L. E., Heding L., Schaller J. P. Immunization against feline oncarnavirus disease using a killed tumor cell vaccine. Cancer Res. 1976 Oct;36(10):3642–3646. [PubMed] [Google Scholar]
  14. Olsen R. G., Hoover E. A., Schaller J. P., Mathes L. E., Wolff L. H. Abrogation of resistance to feline oncornavirus disease by immunization with killed feline leukemia virus. Cancer Res. 1977 Jul;37(7 Pt 1):2082–2085. [PubMed] [Google Scholar]
  15. Olsen R. G., Milo G. E., Schaller J. P., Mathes L. E., Heding L., Yohn D. S. Influence of culture conditions of growth of FL-74 cells and feline oncornavirus cell membrane associated antigen production. In Vitro. 1976 Jan;12(1):37–43. doi: 10.1007/BF02832791. [DOI] [PubMed] [Google Scholar]
  16. Pedersen N. C., Theilen G. H., Werner L. L. Safety and efficacy studies of live- and killed-feline leukemia virus vaccines. Am J Vet Res. 1979 Aug;40(8):1120–1126. [PubMed] [Google Scholar]
  17. Rojko J. L., Hoover E. A., Mathes L. E., Krakowka S., Olsen R. G. Influence of adrenal corticosteroids on the susceptibility of cats to feline leukemia virus infection. Cancer Res. 1979 Sep;39(9):3789–3791. [PubMed] [Google Scholar]
  18. Salerno R. A., Larson V. M., Phelps A. H., Hilleman M. R. Infection and immunization of cats with the Kawakami-Theilen strain of feline leukemia virus. Proc Soc Exp Biol Med. 1979 Jan;160(1):18–23. doi: 10.3181/00379727-160-40379. [DOI] [PubMed] [Google Scholar]
  19. Salerno R. A., Lehman E. D., Larson V. M., Hilleman M. R. Feline leukemia virus envelope glycoprotein vaccine: preparation and evaluation of immunizing potency in guinea pig and cat. J Natl Cancer Inst. 1978 Dec;61(6):1487–1493. [PubMed] [Google Scholar]
  20. Schaller J. P., Hoover E. A., Olsen R. G. Active and passive immunization of cats with inactivated feline oncornaviruses. J Natl Cancer Inst. 1977 Nov;59(5):1441–1450. doi: 10.1093/jnci/59.5.1441. [DOI] [PubMed] [Google Scholar]
  21. Schaller J. P., Olsen R. G. Determination of subgroup-specific feline oncornavirus-neutralizing antibody. Infect Immun. 1975 Dec;12(6):1405–1410. doi: 10.1128/iai.12.6.1405-1410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stephenson J. R., Khan A. S., Sliski A. H., Essex M. Feline oncornavirus-associated cell membrane antigen: evidence for an immunologically crossreactive feline sarcoma virus-coded protein. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5608–5612. doi: 10.1073/pnas.74.12.5608. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Theilen G. H., Kawakami T. G., Rush J. D., Munn R. J. Replication of cat leukemia virus in cell suspension cultures. Nature. 1969 May 10;222(5193):589–590. doi: 10.1038/222589b0. [DOI] [PubMed] [Google Scholar]
  24. Wolff L. H., Mathes L. E., Olsen R. G. Recovery of soluble feline oncornavirus-associated cell membrane antigen from large volumes of tissue culture fluids. J Immunol Methods. 1979;26(2):151–156. doi: 10.1016/0022-1759(79)90078-4. [DOI] [PubMed] [Google Scholar]
  25. Yohn D. S., Olsen R. G., Schaller J. P., Hoover E. A., Mathes L. E., Heding L., Davis G. W. Experimental oncornavirus vaccines in the cat. Cancer Res. 1976 Feb;36(2 Pt 2):646–651. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES