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. 1981 Dec;34(3):1065–1067. doi: 10.1128/iai.34.3.1065-1067.1981

Surface expression of a nonstructural antigen on influenza A virus-infected cells.

M W Shaw, E W Lamon, R W Compans
PMCID: PMC350976  PMID: 7037639

Abstract

Rabbit antiserum to the influenza A virus nonstructural protein, NS1, was used for indirect immunofluorescence studies of infected cells. Nonstructural antigens were detected on surfaces of P815 cells as early as 4 h after infection with A/WSN/40 virus. Adsorption of the serum with virion structural proteins did not affect the observed fluorescence, and a progressive increase in surface fluorescence at later times postinfection indicated that the surface antigen was newly synthesized during the replication cycle.

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Selected References

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  1. Biddison W. E., Doherty P. C., Webster R. G. Antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with type A influenza viruses. J Exp Med. 1977 Sep 1;146(3):690–697. doi: 10.1084/jem.146.3.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Braciale T. J. Immunologic recognition of influenza virus-infected cells. II. Expression of influenza A matrix protein on the infected cell surface and its role in recognition by cross-reactive cytotoxic T cells. J Exp Med. 1977 Sep 1;146(3):673–689. doi: 10.1084/jem.146.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Choppin P. W. Replication of influenza virus in a continuous cell line: high yield of infective virus from cells inoculated at high multiplicity. Virology. 1969 Sep;39(1):130–134. doi: 10.1016/0042-6822(69)90354-7. [DOI] [PubMed] [Google Scholar]
  5. Dimmock N. J. New virus-specific antigens in cells infected with influenza virus. Virology. 1969 Oct;39(2):224–234. doi: 10.1016/0042-6822(69)90042-7. [DOI] [PubMed] [Google Scholar]
  6. Doherty P. C., Effros R. B., Bennink J. Heterogeneity of the cytotoxic response of thymus-derived lymphocytes after immunization with influenza viruses. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1209–1213. doi: 10.1073/pnas.74.3.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Effros R. B., Doherty P. C., Gerhard W., Bennink J. Generation of both cross-reactive and virus-specific T-cell populations after immunization with serologically distinct influenza A viruses. J Exp Med. 1977 Mar 1;145(3):557–568. doi: 10.1084/jem.145.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hackett C. J., Askonas B. A., Webster R. G., van Wyke K. Quantitation of influenza virus antigens on infected target cells and their recognition by cross-reactive cytotoxic T cells. J Exp Med. 1980 May 1;151(5):1014–1025. doi: 10.1084/jem.151.5.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  10. Koszinowski U. H., Allen H., Gething M. J., Waterfield M. D., Klenk H. D. Recognition of viral glycoproteins by influenza A-specific cross-reactive cytolytic T lymphocytes. J Exp Med. 1980 Apr 1;151(4):945–958. doi: 10.1084/jem.151.4.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Morrongiello M. P., Dales S. Characterization of cytoplasmic inclusions formed during influenza/WSN virus infection of chick embryo fibroblast cells. Intervirology. 1977;8(5):281–293. doi: 10.1159/000148903. [DOI] [PubMed] [Google Scholar]
  13. Reiss C. S., Schulman J. L. Influenza type A virus M protein expression on infected cells is responsible for cross-reactive recognition by cytotoxic thymus-derived lymphocytes. Infect Immun. 1980 Aug;29(2):719–723. doi: 10.1128/iai.29.2.719-723.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHULMAN J. L., KILBOURNE E. D. INDUCTION OF PARTIAL SPECIFIC HETEROTYPIC IMMUNITY IN MICE BY A SINGLE INFECTION WITH INFLUENZA A VIRUS. J Bacteriol. 1965 Jan;89:170–174. doi: 10.1128/jb.89.1.170-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shaw M. W., Compans R. W. Isolation and characterization of cytoplasmic inclusions from influenza A virus-infected cells. J Virol. 1978 Feb;25(2):608–615. doi: 10.1128/jvi.25.2.608-615.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stohrer R., Hunter E. Inhibition of Rous sarcoma virus replication by 2-deoxyglucose and tunicamycin: identification of an unglycosylated env gene product. J Virol. 1979 Nov;32(2):412–419. doi: 10.1128/jvi.32.2.412-419.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Virelizier J. L., Allison A. C., Oxford J. S., Schild G. C. Early presence of ribonucleoprotein antigen on surface of influenza virus-infected cells. Nature. 1977 Mar 3;266(5597):52–54. doi: 10.1038/266052a0. [DOI] [PubMed] [Google Scholar]
  18. Zweerink H. J., Courtneidge S. A., Skehel J. J., Crumpton M. J., Askonas B. A. Cytotoxic T cells kill influenza virus infected cells but do not distinguish between serologically distinct type A viruses. Nature. 1977 May 26;267(5609):354–356. doi: 10.1038/267354a0. [DOI] [PubMed] [Google Scholar]

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