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. 1982 Jan;35(1):138–142. doi: 10.1128/iai.35.1.138-142.1982

Preemption of Streptococcus mutans 10449S colonization by its mutant 805.

J M Tanzer, J Fisher, M L Freedman
PMCID: PMC351007  PMID: 6459292

Abstract

Oral infection of rats with Streptococcus mutans mutant 805 was used to prevent the establishment of its highly virulent wild-type progenitor NCTC 10449S. The dose of wild-type cells required to colonize 100% of the specific pathogen-free Osborne-Mendel rats (21 to 43 days old) consuming caries test diet 2000 was greater than 4 x 10(5) but less than 4 x 10(6) cells. Therefore, the latter dose was used to challenge rats which had already been colonized by an oral dose of about 6 x 10(8) cells of mutant 805. This prior infection by 805 either completely protected rats from subsequent colonization by wild-type cells or greatly delayed and diminished their emergence. Rats in which wild-type cells became established showed much lower percentages of wild-type cells in their total recoverable floras than did rats that were not first infected by the mutant. Large doses of mutant 805, however, did not displace wild-type cells from rats once it became established. There was no evidence of reversion of the mutant, which is defective in intracellular polysaccharide synthesis and hence is less virulent than wild-type cells. The data indicate that the S. mutans cell which first colonizes rats gains the strongest ecological position and is difficult to displace. Also, they suggest the possible prophylactic utility of infection by this mutant of S. mutans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birkhed D., Tanzer J. M. Glycogen synthesis pathway in Streptococcus mutans strain NCTC 10449S and its glycogen synthesis-defective mutant 805. Arch Oral Biol. 1979;24(1):67–73. doi: 10.1016/0003-9969(79)90177-8. [DOI] [PubMed] [Google Scholar]
  2. Carlos J. P., Gittelsohn A. M. Longitudinal studies of the natural history of caries. II. A life-table study of caries incidence in the permanent teeth. Arch Oral Biol. 1965 Sep-Oct;10(5):739–751. doi: 10.1016/0003-9969(65)90127-5. [DOI] [PubMed] [Google Scholar]
  3. Davidson J. N., Hirsh D. C. Bacterial competition as a means of preventing neonatal diarrhea in pigs. Infect Immun. 1976 Jun;13(6):1773–1774. doi: 10.1128/iai.13.6.1773-1774.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzgerald R. J., Larson R. H. Age and caries susceptibility in gnotobiotic rats. Helv Odontol Acta. 1967 Apr;11(1):49–52. [PubMed] [Google Scholar]
  5. Hillman J. D. Lactate dehydrogenase mutants of Streptococcus mutans: isolation and preliminary characterization. Infect Immun. 1978 Jul;21(1):206–212. doi: 10.1128/iai.21.1.206-212.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huber G., Van Houte J., Edelstein S. Relationship between the populations of Streptococcus mutans in the mouth and feces of conventional Sprague-Dawley rats. J Dent Res. 1977 Dec;56(12):1614–1619. doi: 10.1177/00220345770560123401. [DOI] [PubMed] [Google Scholar]
  7. JORDAN H. V., FITZGERALD R. J., BOWLER A. E. Inhibition of experimental caries by sodium metabisulfite and its effect on the growth and metabolism of selected bacteria. J Dent Res. 1960 Jan-Feb;39:116–123. doi: 10.1177/00220345600390010501. [DOI] [PubMed] [Google Scholar]
  8. Johnson C. P., Gross S. M., Hillman J. D. Cariogenic potential in vitro in man and in vivo in the rat of lactate dehydrogenase mutants of Streptococcus mutans. Arch Oral Biol. 1980;25(11-12):707–713. doi: 10.1016/0003-9969(80)90124-7. [DOI] [PubMed] [Google Scholar]
  9. KEYES P. H., JORDAN H. V. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. doi: 10.1016/0003-9969(64)90024-x. [DOI] [PubMed] [Google Scholar]
  10. Sanders E. Bacterial interference. I. Its occurrence among the respiratory tract flora and characterization of inhibition of group A streptococci by viridans streptococci. J Infect Dis. 1969 Dec;120(6):698–707. doi: 10.1093/infdis/120.6.698. [DOI] [PubMed] [Google Scholar]
  11. Shinefield H. R., Ribble J. C., Boris M. Bacterial interference between strains of Staphylococcus aureus, 1960 to 1970. Am J Dis Child. 1971 Feb;121(2):148–152. doi: 10.1001/archpedi.1971.02100130102013. [DOI] [PubMed] [Google Scholar]
  12. Tanzer J. M. Essential dependence of smooth surface caries on, and augmentation of fissure caries by, sucrose and Streptococcus mutans infection. Infect Immun. 1979 Aug;25(2):526–531. doi: 10.1128/iai.25.2.526-531.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tanzer J. M., Freedman M. L., Fitzgerald R. J., Larson R. H. Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):197–203. doi: 10.1128/iai.10.1.197-203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tanzer J. M., Freedman M. L. Genetic alterations of Streptococcus mutans' virulence. Adv Exp Med Biol. 1978;107:661–672. doi: 10.1007/978-1-4684-3369-2_75. [DOI] [PubMed] [Google Scholar]

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