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. 1982 Jan;35(1):187–192. doi: 10.1128/iai.35.1.187-192.1982

Transfer of resistance with syphilitic immune cells: lack of correlation with mitogenic activity.

R F Schell, J L LeFrock, J K Chan
PMCID: PMC351014  PMID: 7054121

Abstract

Hamsters infected intradermally with Treponema pallidum Bosnia A develop extensive chronic skin lesions, usually accompanied by metastatic lesions involving the paws, lips, and anal region and by lymph nodes teeming with treponemes. Throughout the course of syphilitic infection, cells from the inguinal lymph nodes responded poorly to stimulation with suboptimal, optimal, or supraoptimal concentrations of concanavalin A, phytohemagglutinin P, or lipopolysaccharide. The response of syphilitic spleen cells was variable. Depression of lymphocyte reactivity to mitogens preceded clinical signs of infection and correlated well with the chronicity of syphilitic infection. When syphilitic hamsters were treated with a curative dose of penicillin, their mitogenic responses returned to normal or were slightly elevated. No correlation existed between mitogenic activity and the ability of lymphoid cells to induce an effective immune response when transferred to normal recipients. No significant differences in protection were detected among recipients of immune cells with or without activity to mitogens. These results demonstrate that lymphocyte transformation by mitogens in vitro is not a measure of effective treponemicidal activity and so may not be a valid indicator of the protective immune status of syphilitic animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderete J. F., Baseman J. B. Surface-associated host proteins on virulent Treponema pallidum. Infect Immun. 1979 Dec;26(3):1048–1056. doi: 10.1128/iai.26.3.1048-1056.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baseman J. B., Hayes E. C. Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum. J Exp Med. 1980 Mar 1;151(3):573–586. doi: 10.1084/jem.151.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bey R. F., Johnson R. C., Fitzgerald T. J. Suppression of lymphocyte response to concanavalin A by mucopolysaccharide material from Treponema pallidum-infected rabbits. Infect Immun. 1979 Oct;26(1):64–69. doi: 10.1128/iai.26.1.64-69.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duthu A., Haddada H., Le Goffic N., de Vaux-Saint-Cyr C. Protein A : a B cell mitogen for hamster splenic lymphoid cells. Dev Comp Immunol. 1980 Spring;4(2):331–339. doi: 10.1016/s0145-305x(80)80036-x. [DOI] [PubMed] [Google Scholar]
  5. Fitzgerald T. J., Johnson R. C. Influence of testicular fluid infected with Treponema pallidum on intradermal lesions. Br J Vener Dis. 1980 Jun;56(3):125–128. doi: 10.1136/sti.56.3.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fitzgerald T. J., Johnson R. C. Surface mucopolysaccharides of Treponema pallidum. Infect Immun. 1979 Apr;24(1):244–251. doi: 10.1128/iai.24.1.244-251.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Friedmann P. S. Cell-mediated immunological reactivity in neonates and infants with congenital syphilis. Clin Exp Immunol. 1977 Nov;30(2):271–276. [PMC free article] [PubMed] [Google Scholar]
  8. Friedmann P. S., Turk J. L. The role of cell-mediated immune mechanisms in syphilis in Ethiopia. Clin Exp Immunol. 1978 Jan;31(1):59–65. [PMC free article] [PubMed] [Google Scholar]
  9. From E., Thestrup-Pedersen K., Thulin H. Reactivity of lymphocytes from patients with syphilis towards T. pallidum antigen in the leucocyte migration and lymphocyte transformation tests. Br J Vener Dis. 1976 Aug;52(4):224–229. doi: 10.1136/sti.52.4.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green F., 3rd, Balish E. Suppression of in vitro lymphocyte transformation during an experimental dermatophyte infection. Infect Immun. 1979 Nov;26(2):554–562. doi: 10.1128/iai.26.2.554-562.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HOLLANDER D. H., TURNER T. B. The role of temperature in experimental treponemal infection. Am J Syph Gonorrhea Vener Dis. 1954 Nov;38(6):489–505. [PubMed] [Google Scholar]
  12. Loh L., Hudson J. B. Immunosuppressive effect of murine cytomegalovirus. Infect Immun. 1980 Jan;27(1):54–60. doi: 10.1128/iai.27.1.54-60.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maret S. M., Baseman J. B., Folds J. D. Cell-mediated immunity in Treponema pallidum infected rabbits: in vitro response of splenic and lymph node lymphocytes to mitogens and specific antigens. Clin Exp Immunol. 1980 Jan;39(1):38–43. [PMC free article] [PubMed] [Google Scholar]
  14. Musher D. M., Schell R. F., Knox J. M. In vitro lymphocyte response to Treponema refringens im human syphilis. Infect Immun. 1974 Apr;9(4):654–657. doi: 10.1128/iai.9.4.654-657.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pavia C. S., Baseman J. B., Folds J. D. Selective response of lymphocytes from Treponema pallidum-infected rabbits to mitogens and Treponema reiteri. Infect Immun. 1977 Feb;15(2):417–422. doi: 10.1128/iai.15.2.417-422.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pavia C. S., Folds J. D., Baseman J. B. Depression of lymphocyte response to concanavalin A in rabbits infected with Treponema pallidum (Nichols strain). Infect Immun. 1976 Jul;14(1):320–322. doi: 10.1128/iai.14.1.320-322.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schell R. F., Chan J. K., Le Frock J. L. Endemic syphilis: passive transfer of resistance with serum and cells in hamsters. J Infect Dis. 1979 Sep;140(3):378–383. doi: 10.1093/infdis/140.3.378. [DOI] [PubMed] [Google Scholar]
  18. Schell R. F., Chan J. K., LeFrock J. L., Bagasra O. Endemic syphilis: transfer of resistance to Treponema pallidum strain Bosnia A in hamsters with a cell suspension enriched in thymus-derived cells. J Infect Dis. 1980 Jun;141(6):752–758. doi: 10.1093/infdis/141.6.752. [DOI] [PubMed] [Google Scholar]
  19. Schell R. F., LeFrock J. L., Chan J. K., Bagasra O. LSH hamster model of syphilitic infection. Infect Immun. 1980 Jun;28(3):909–913. doi: 10.1128/iai.28.3.909-913.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor D. W., Crum S. R., Kramer K. J., Siddiqui W. A. Alterations in the distribution and proliferative responses of rhesus monkey peripheral blood and spleen cells during malaria (Plasmodium knowlesi) infection. Infect Immun. 1980 May;28(2):502–507. doi: 10.1128/iai.28.2.502-507.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Turcotte R. Influence of route of Mycobacterium lepraemurium injection on susceptibility to mouse leprosy and on lymphoblastic transformation. Infect Immun. 1980 Jun;28(3):660–668. doi: 10.1128/iai.28.3.660-668.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wicher V., Wicher K. In vitro cell response of Treponema pallidum-infected rabbits. I. Lymphocyte transformation. Clin Exp Immunol. 1977 Sep;29(3):480–486. [PMC free article] [PubMed] [Google Scholar]

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