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. 1982 Jan;35(1):206–212. doi: 10.1128/iai.35.1.206-212.1982

Chemiluminescence of human neutrophils induced by soluble stimuli: effect of divalent cations.

L R DeChatelet, P S Shirley
PMCID: PMC351017  PMID: 6797947

Abstract

The effect of three soluble stimuli, phorbol myristate acetate, concanavalin A, and the calcium ionophore A23187, on the luminol-enhanced chemiluminescence of human neutrophils was investigated. All three stimuli elicited a strong burst of chemiluminescence which was dose dependent. The effect of phorbol myristate acetate was independent of the presence of divalent cations in the medium and, in fact, was greater in the presence of ethylene glycol-bis(beta-aminoethyl ether)-N,N-tetraacetic acid. The effect of concanavalin A was greatly stimulated by the presence of calcium in the medium, although some reaction was observed in the absence of this cation. In contrast, chemiluminescence induced by A23187 was absolutely dependent upon the presence of extracellular calcium. These results suggest that the mobilization of calcium into the cell is a sufficient, but not necessary, stimulus for initiation of the respiratory burst. Compounds such as phorbol myristate acetate, which act independently of extracellular divalent cations, may function by altering the intracellular ratio of bound/free calcium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. C., Stjernholm R. L., Steele R. H. Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity. Biochem Biophys Res Commun. 1972 May 26;47(4):679–684. doi: 10.1016/0006-291x(72)90545-1. [DOI] [PubMed] [Google Scholar]
  2. Cheson B. D., Christensen R. L., Sperling R., Kohler B. E., Babior B. M. The origin of the chemiluminescence of phagocytosing granulocytes. J Clin Invest. 1976 Oct;58(4):789–796. doi: 10.1172/JCI108530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen M. S., Metcalf J. A., Root R. K. Regulation of oxygen metabolism in human granulocytes: relationship between stimulus binding and oxidative response using plant lectins as probes. Blood. 1980 Jun;55(6):1003–1010. [PubMed] [Google Scholar]
  4. DeChatelet L. R., Cooper M. R. A modified procedure for the determination of leukocyte alkaline phosphatase. Biochem Med. 1970 Aug;4(1):61–68. doi: 10.1016/0006-2944(70)90103-1. [DOI] [PubMed] [Google Scholar]
  5. DeChatelet L. R. Initiation of the respiratory burst in human polymorphonuclear neutrophils: a critical review. J Reticuloendothel Soc. 1978 Jul;24(1):73–91. [PubMed] [Google Scholar]
  6. DeChatelet L. R., Shirley P. S., Johnston R. B., Jr Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood. 1976 Apr;47(4):545–554. [PubMed] [Google Scholar]
  7. Goldstein I. M., Horn J. K., Kaplan H. B., Weissmann G. Calcium-induced lysozyme secretion from human polymorphonuclear leukocytes. Biochem Biophys Res Commun. 1974 Sep 23;60(2):807–812. doi: 10.1016/0006-291x(74)90312-x. [DOI] [PubMed] [Google Scholar]
  8. Hoffstein S. T. Ultrastructural demonstration of calcium loss from local regions of the plasma membrane of surface-stimulated human granulocytes. J Immunol. 1979 Sep;123(3):1395–1402. [PubMed] [Google Scholar]
  9. Korchak H. M., Weissmann G. Changes in membrane potential of human granulocytes antecede the metabolic responses to surface stimulation. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3818–3822. doi: 10.1073/pnas.75.8.3818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McPhail L. C., DeChatelet L. R., Johnston R. B., Jr Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events. J Clin Invest. 1979 Apr;63(4):648–655. doi: 10.1172/JCI109347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Naccache P. H., Volpi M., Showell H. J., Becker E. L., Sha'afi R. I. Chemotactic factor-induced release of membrane calcium in rabbit neutrophils. Science. 1979 Feb 2;203(4379):461–463. doi: 10.1126/science.760200. [DOI] [PubMed] [Google Scholar]
  12. Nelson R. D., Herron M. J., Schmidtke J. R., Simmons R. L. Chemiluminescence response of human leukocytes: influence of medium components on light production. Infect Immun. 1977 Sep;17(3):513–520. doi: 10.1128/iai.17.3.513-520.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Newburger P. E., Chovaniec M. E., Cohen H. J. Activity and activation of the granulocyte superoxide-generating system. Blood. 1980 Jan;55(1):85–92. [PubMed] [Google Scholar]
  14. Prendergast E., Proctor R. A. Simple procedure for measuring neutrophil chemiluminescence. J Clin Microbiol. 1981 Feb;13(2):390–392. doi: 10.1128/jcm.13.2.390-392.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schell-Frederick E. Stimulation of the oxidative metabolism of polymorphonuclear leucocytes by the calcium ionophore A23187. FEBS Lett. 1974 Nov 1;48(1):37–40. doi: 10.1016/0014-5793(74)81056-2. [DOI] [PubMed] [Google Scholar]
  16. Smith R. J., Ignarro L. J. Bioregulation of lysosomal enzyme secretion from human neutrophils: roles of guanosine 3':5'-monophosphate and calcium in stimulus-secretion coupling. Proc Natl Acad Sci U S A. 1975 Jan;72(1):108–112. doi: 10.1073/pnas.72.1.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takeshige K., Minakami S. Involvement of calmodulin in phagocytotic respiratory burst of leukocytes. Biochem Biophys Res Commun. 1981 Mar 31;99(2):484–490. doi: 10.1016/0006-291x(81)91770-8. [DOI] [PubMed] [Google Scholar]
  18. Westrick M. A., Shirley P. S., DeChatelet L. R. Generation of chemiluminescence by human neutrophils exposed to soluble stimuli of oxidative metabolism. Infect Immun. 1980 Nov;30(2):385–390. doi: 10.1128/iai.30.2.385-390.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilson M. E., Trush M. A., van Dyke K., Neal W. Induction of chemiluminescence in human polymorphonuclear leukocytes by the calcium ionophore A23187. FEBS Lett. 1978 Oct 15;94(2):387–390. doi: 10.1016/0014-5793(78)80984-3. [DOI] [PubMed] [Google Scholar]
  20. Zabucchi G., Romeo D. The dissociation of exocytosis and respiratory stimulation in leucocytes by ionophores. Biochem J. 1976 May 15;156(2):209–213. doi: 10.1042/bj1560209. [DOI] [PMC free article] [PubMed] [Google Scholar]

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