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. 1982 Jan;35(1):270–275. doi: 10.1128/iai.35.1.270-275.1982

Serum resistance among Escherichia coli strains causing urinary tract infection in relation to O type and the carriage of hemolysin, colicin, and antibiotic resistance determinants.

C Hughes, R Phillips, A P Roberts
PMCID: PMC351025  PMID: 7033137

Abstract

The sensitivity to normal human serum of 91 smooth strains of Escherichia coli isolated from urinary tract infections was determined. Production of hemolysin, which was common and associated primarily with the types O4, O6, O18, and O75, was significantly correlated with high levels of serum resistance, both within the total population and within individual O types. In contrast, serum resistance was not significantly associated with antibiotic resistance (whether transmissible or not), with colicinogeny in general, or with colicin V production in particular. This indicates that the carriage of R and ColV plasmids, shown previously to be capable of conferring increased levels of serum resistance on individual strains of E. coli isolated from other sources, does not play an important part in determining the serum sensitivity of the E. coli population involved in urinary tract infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns M. M., Davies D. L., Hardy K. G. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature. 1979 Jun 28;279(5716):778–781. doi: 10.1038/279778a0. [DOI] [PubMed] [Google Scholar]
  2. Cooke E. M., Ewins S. P. Properties of strains of Escherichia coli isolated from a variety of sources. J Med Microbiol. 1975 Feb;8(1):107–111. doi: 10.1099/00222615-8-1-107. [DOI] [PubMed] [Google Scholar]
  3. Cooke E. M. Properties of strains of Escherichia coli isolated from the faeces of patients with ulcerative colitis, patients with acute diarrhoea and normal persons. J Pathol Bacteriol. 1968 Jan;95(1):101–113. doi: 10.1002/path.1700950112. [DOI] [PubMed] [Google Scholar]
  4. Davies D. L., Falkiner F. R., Hardy K. G. Colicin V production by clinical isolates of Escherichia coli. Infect Immun. 1981 Feb;31(2):574–579. doi: 10.1128/iai.31.2.574-579.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Durack D. T., Beeson P. B. Protective role of complement in experimental Escherichia coli endocarditis. Infect Immun. 1977 Apr;16(1):213–217. doi: 10.1128/iai.16.1.213-217.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feingold D. S. The serum bactericidal reaction. IV. Phenotypic conversion of Escherichia coli from serum-resistance to serum-sensitivity by diphenylamine. J Infect Dis. 1969 Oct;120(4):437–444. doi: 10.1093/infdis/120.4.437. [DOI] [PubMed] [Google Scholar]
  7. Glynn A. A., Howard C. J. The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology. 1970 Mar;18(3):331–346. [PMC free article] [PubMed] [Google Scholar]
  8. Goebel W., Royer-Pokora B., Lindenmaier W., Bujard H. Plasmids controlling synthesis of hemolysin in Escherichia coli: molecular properties. J Bacteriol. 1974 Jun;118(3):964–973. doi: 10.1128/jb.118.3.964-973.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gower P. E., Taylor P. W., Koutsaimanis K. G., Roberts A. P. Serum bactericidal activity in patients with upper and lower urinary tract infections. Clin Sci. 1972 Jul;43(1):13–22. doi: 10.1042/cs0430013. [DOI] [PubMed] [Google Scholar]
  10. Hildebrandt J. F., Mayer L. W., Wang S. P., Buchanan T. M. Neisseria gonorrhoeae acquire a new principal outer-membrane protein when transformed to resistance to serum bactericidal activity. Infect Immun. 1978 Apr;20(1):267–272. doi: 10.1128/iai.20.1.267-272.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Howard C. J., Glynn A. A. The virulence for mice of strains of Escherichia coli related to the effects of K antigens on their resistance to phagocytosis and killing by complement. Immunology. 1971 May;20(5):767–777. [PMC free article] [PubMed] [Google Scholar]
  12. Hughes C., Bauer E., Roberts A. P. Spread of R-plasmids among Escherichia coli causing urinary tract infections. Antimicrob Agents Chemother. 1981 Oct;20(4):496–502. doi: 10.1128/aac.20.4.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hughes V., Le Grice S., Hughes C., Meynell G. G. Two major groups of colicin factors: their molecular weights. Mol Gen Genet. 1978 Feb 16;159(2):219–221. doi: 10.1007/BF00270897. [DOI] [PubMed] [Google Scholar]
  14. McCabe W. R., Kaijser B., Olling S., Uwaydah M., Hanson L. A. Escherichia coli in bacteremia: K and O antigens and serum sensitivity of strains from adults and neonates. J Infect Dis. 1978 Jul;138(1):33–41. doi: 10.1093/infdis/138.1.33. [DOI] [PubMed] [Google Scholar]
  15. Minshew B. H., Jorgensen J., Swanstrum M., Grootes-Reuvecamp G. A., Falkow S. Some characteristics of Escherichia coli strains isolated from extraintestinal infections of humans. J Infect Dis. 1978 May;137(5):648–654. doi: 10.1093/infdis/137.5.648. [DOI] [PubMed] [Google Scholar]
  16. Moll A., Manning P. A., Timmis K. N. Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli. Infect Immun. 1980 May;28(2):359–367. doi: 10.1128/iai.28.2.359-367.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Noegel A., Rdest U., Goebel W. Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli. J Bacteriol. 1981 Jan;145(1):233–247. doi: 10.1128/jb.145.1.233-247.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Olling S., Hanson L. A., Holmgren J., Jodal U., Lincoln K., Lindberg U. The bactericidal effect of normal human serum on E. coli strains from normals and from patients with urinary tract infections. Infection. 1973;1(1):24–28. doi: 10.1007/BF01638251. [DOI] [PubMed] [Google Scholar]
  19. Petrocheilou V., Richmond M. H. Distribution of R plasmids among the Q-antigen types of Escherichia coli isolated from various clinical sources. Antimicrob Agents Chemother. 1976 Jan;9(1):1–5. doi: 10.1128/aac.9.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Roberts A. P., Phillips R. Bacteria causing symptomatic urinary tract infection or asymptomatic bacteriuria. J Clin Pathol. 1979 May;32(5):492–496. doi: 10.1136/jcp.32.5.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Simberkoff M. S., Ricupero I., Rahal J. J., Jr Host resistance to Serratia marcescens infection: serum bactericidal activity and phagocytosis by normal blood leukocytes. J Lab Clin Med. 1976 Feb;87(2):206–217. [PubMed] [Google Scholar]
  22. Smith H. W., Halls S. The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production. J Gen Microbiol. 1967 Apr;47(1):153–161. doi: 10.1099/00221287-47-1-153. [DOI] [PubMed] [Google Scholar]
  23. Taylor P. W. Genetical studies of serum resistance in Escherichia coli. J Gen Microbiol. 1975 Jul;89(1):57–66. doi: 10.1099/00221287-89-1-57. [DOI] [PubMed] [Google Scholar]
  24. Taylor P. W., Hughes C. Plasmid carriage and the serum sensitivity of enterobacteria. Infect Immun. 1978 Oct;22(1):10–17. doi: 10.1128/iai.22.1.10-17.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taylor P. W. Immunochemical investigations on lipopolysaccharides and acidic polysaccharides from serum-sensitive and serum-resistant strains of Escherichia coli isolated from urinary-tract infections. J Med Microbiol. 1976 Nov;9(4):405–421. doi: 10.1099/00222615-9-4-405. [DOI] [PubMed] [Google Scholar]
  26. Taylor P. W., Koutsaimanis K. G. Experimental Escherichia coli urinary infection in the rat. Kidney Int. 1975 Oct;8(4):233–238. doi: 10.1038/ki.1975.106. [DOI] [PubMed] [Google Scholar]
  27. Taylor P. W., Parton R. A protein factor associated with serum resistance in Escherichia coli. J Med Microbiol. 1977 May;10(2):225–232. doi: 10.1099/00222615-10-2-225. [DOI] [PubMed] [Google Scholar]
  28. Taylor P. W., Robinson M. K. Determinants that increase the serum resistance of Escherichia coli. Infect Immun. 1980 Jul;29(1):278–280. doi: 10.1128/iai.29.1.278-280.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Taylor P. W. Sensitivity of some smooth strains of Escherichia coli to the bactericidal action of normal human serum. J Clin Pathol. 1974 Aug;27(8):626–629. doi: 10.1136/jcp.27.8.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. de la Cruz F., Müller D., Ortiz J. M., Goebel W. Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups. J Bacteriol. 1980 Aug;143(2):825–833. doi: 10.1128/jb.143.2.825-833.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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