Figure 8. Elba proteins are bound to Fab-7 in early but not late embryos.
(A) and (B) Elba1 and 2 ChIPs. Early (2–5 hr) or late (9–12 hr) embryos were cross-linked and after processing immunoprecipitated with Elba1 or Elba2 antibodies, or pre-immune serum. Sequences from the pHS1 region of Fab-7 or control twine and Sex-lethal sequences were detected by qPCR. The y-axis shows the average immune/preimmune ratio. *p<0.05. (C) Model showing binding and enhancer blocking by the Elba complex in early but not late embryos. GAF: GAGA factor and its binding sites.