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. 1982 Feb;35(2):396–401. doi: 10.1128/iai.35.2.396-401.1982

Escherichia coli-associated porcine neonatal diarrhea: antibacterial activities of colostrum from genetically susceptible and resistant sows.

R Sellwood
PMCID: PMC351052  PMID: 7035361

Abstract

Antibacterial properties of colostrum from genetically resistant and susceptible sows in a herd in which only the susceptible sows had acquired natural immunity to K88-positive Escherichia coli have been investigated. Significant differences in antiadhesive and opsonic activities occurred. Colostrum from susceptible sows inhibited the binding of 125I-labeled K88 antigen to brush borders significantly better than did the colostrum from resistant dams. Colostrum from susceptible dams effected more efficient in vitro opsonic phagocytosis and killing of K88 E. coli than did colostrum from resistant dams. Differences in bactericidal properties of colostrum between the two groups of pigs were not significant. Fractionation of colostrum from susceptible dams by gel filtration and ion-exchange chromatography revealed that the fractions rich in immunoglobulin M had the highest opsonic activity, whereas those containing predominantly immunoglobulins G and A were of lower activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bellamy J. E., Nielsen N. O. Immune-mediated emigration of neutrophils into the lumen of the small intestine. Infect Immun. 1974 Apr;9(4):615–619. doi: 10.1128/iai.9.4.615-619.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bourne F. J., Newby T. J., Chidlow J. W. The influence of route of vaccination on the systemic and local immune response in the pig. Res Vet Sci. 1975 May;18(3):244–248. [PubMed] [Google Scholar]
  3. Carlson G. P., Kaneko J. J. Isolation of leukocytes from bovine peripheral blood. Proc Soc Exp Biol Med. 1973 Mar;142(3):853–856. doi: 10.3181/00379727-142-37131. [DOI] [PubMed] [Google Scholar]
  4. Chidlow J. W., Porter P. Intestinal defence of the neonatal pig: interrelationship of gut and mammary function providing surface immunity against colibacillosis. Vet Rec. 1979 Jun 2;104(22):496–500. doi: 10.1136/vr.104.22.496. [DOI] [PubMed] [Google Scholar]
  5. Chidlow J. W., Porter P. The role of oral immunisation in stimulating Escherichia coli antibody of the IgM class in porcine colostrum. Res Vet Sci. 1978 Mar;24(2):254–257. [PubMed] [Google Scholar]
  6. Chidlow J. W., Porter P. Uptake of maternal antibody by the neonatal pig following intramuscular and intramammary vaccination of the preparturient sow. Res Vet Sci. 1977 Sep;23(2):185–190. [PubMed] [Google Scholar]
  7. Horwitz M. A., Silverstein S. C. Influence of the Escherichia coli capsule on complement fixation and on phagocytosis and killing by human phagocytes. J Clin Invest. 1980 Jan;65(1):82–94. doi: 10.1172/JCI109663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones G. W., Rutter J. M. Contribution of the K88 antigen of Escherichia coli to enteropathogenicity; protection against disease by neutralizing the adhesive properties of K88 antigen. Am J Clin Nutr. 1974 Dec;27(12):1441–1449. doi: 10.1093/ajcn/27.12.1441. [DOI] [PubMed] [Google Scholar]
  9. Kohler E. M., Cross R. F., Bohl E. H. Protection against neonatal enteric colibacillosis in pigs suckling orally vaccinated sows. Am J Vet Res. 1975 Jun;36(6):757–764. [PubMed] [Google Scholar]
  10. Kohler E. M. Protection of pigs against neonatal enteric colibacillosis with colostrum and milk from orally vaccinated sows. Am J Vet Res. 1974 Mar;35(3):331–338. [PubMed] [Google Scholar]
  11. Nagy B., Moon H. W., Isaacson R. E., To C. C., Brinton C. C. Immunization of suckling pigs against enteric enterotoxigenic Escherichia coli infection by vaccinating dams with purified pili. Infect Immun. 1978 Jul;21(1):269–274. doi: 10.1128/iai.21.1.269-274.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagy L. K., Bhogal B. S., Mackenzie T. Duration of anti-adhesive and bactericidal activities of milk from vaccinated sows on Escherichia coli O149 in the digestive tract of piglets during the nursing period. Res Vet Sci. 1979 Nov;27(3):289–296. [PubMed] [Google Scholar]
  13. Porter P., Kenworthy R., Holme D. W., Horsfield S. Escherichia coli antigens as dietary additives for oral immunisation of pigs: trials with pig creep feeds. Vet Rec. 1973 Jun 16;92(24):630–636. doi: 10.1136/vr.92.24.630. [DOI] [PubMed] [Google Scholar]
  14. Porter P. Transfer of immunoglobulins IgG, IgA and IgM to lacteal secretions in the parturient sow and their absorption by the neonatal piglet. Biochim Biophys Acta. 1969 Jul 1;181(2):381–392. doi: 10.1016/0005-2795(69)90271-2. [DOI] [PubMed] [Google Scholar]
  15. ROBBINS J. B., KENNY K., SUTER E. THE ISOLATION AND BIOLOGICAL ACTIVITIES OF RABBIT GAMMA M- AND GAMMA G-ANTI-SALMONELLA TYPHIMURIUM ANTIBODIES. J Exp Med. 1965 Aug 1;122:385–402. doi: 10.1084/jem.122.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rutter J. M., Anderson J. C. Experimental neonatal diarrhoea caused by an enteropathogenic strain of Escherichia coli in piglets: a study of the disease and the effect of vaccinating the dam. J Med Microbiol. 1972 May;5(2):197–210. doi: 10.1099/00222615-5-2-197. [DOI] [PubMed] [Google Scholar]
  17. Rutter J. M., Jones G. W., Brown G. T., Burrows M. R., Luther P. D. Antibacterial activity in colostrum and milk associated with protection of piglets against enteric disease caused by K88-positive Escherichia coli. Infect Immun. 1976 Mar;13(3):667–676. doi: 10.1128/iai.13.3.667-676.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rutter J. M., Jones G. W. Protection against enteric disease caused by Escherichia coli--a model for vaccination with a virulence determinant? Nature. 1973 Apr 20;242(5399):531–532. doi: 10.1038/242531a0. [DOI] [PubMed] [Google Scholar]
  19. Sellwood R. Escherichia coli diarrhoea in pigs with or without the K88 receptor. Vet Rec. 1979 Sep 8;105(10):228–230. doi: 10.1136/vr.105.10.228. [DOI] [PubMed] [Google Scholar]
  20. Sellwood R. The interaction of the K88 antigen with porcine intestinal epithelial cell brush borders. Biochim Biophys Acta. 1980 Oct 1;632(2):326–335. doi: 10.1016/0304-4165(80)90090-2. [DOI] [PubMed] [Google Scholar]
  21. Solberg C. O. Protection of phagocytized bacteria against antibiotics. A new method for the evaluation of neutrophil granulocyte functions. Acta Med Scand. 1972 May;191(5):383–387. [PubMed] [Google Scholar]
  22. Watson D. L. Antibody response in sows following intramammary or intramuscular immunization with a killed Escherichia coli vaccine. Aust J Exp Biol Med Sci. 1975 Aug;53(4):305–313. doi: 10.1038/icb.1975.33. [DOI] [PubMed] [Google Scholar]
  23. Wilson M. R., Svendsen J. Immunity to Escherichia coli in pigs. The role of milk in protective immunity to e. coli enteritis. Can J Comp Med. 1971 Jul;35(3):239–243. [PMC free article] [PubMed] [Google Scholar]

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