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. 1982 Feb;35(2):461–464. doi: 10.1128/iai.35.2.461-464.1982

Modulatory effect of iron on the pathogenesis of Pseudomonas aeruginosa mouse corneal infections.

D E Woods, P A Sokol, B H Iglewski
PMCID: PMC351062  PMID: 6799404

Abstract

The iron concentration of the culture medium used to prepare the inocula influenced the pathogenesis of mouse corneal infections by Pseudomonas aeruginosa. When the parental strain PAO1 was cultured in high-iron medium (5 micrograms of Fe per ml), it was less virulent than when it was cultured in low-iron medium (0.05 microgram of Fe per ml). The iron concentration of the growth medium had no effect on the virulence of a P. aeruginosa mutant which was resistant to the iron regulation of toxin A yields (PAO-toxFeR-18). A severely defective iron transport mutant, PAO-toxFeR-10, was avirulent regardless of the iron concentration of the growth medium. These studies indicate that both iron acquisition and iron regulation of toxin production are important factors in the determination of P. aeruginosa virulence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bjorn M. J., Iglewski B. H., Ives S. K., Sadoff J. C., Vasil M. L. Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103. Infect Immun. 1978 Mar;19(3):785–791. doi: 10.1128/iai.19.3.785-791.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bjorn M. J., Sokol P. A., Iglewski B. H. Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. J Bacteriol. 1979 Apr;138(1):193–200. doi: 10.1128/jb.138.1.193-200.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cox C. D., Graham R. Isolation of an iron-binding compound from Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):357–364. doi: 10.1128/jb.137.1.357-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cox C. D. Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. J Bacteriol. 1980 May;142(2):581–587. doi: 10.1128/jb.142.2.581-587.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holloway B. W., Krishnapillai V., Morgan A. F. Chromosomal genetics of Pseudomonas. Microbiol Rev. 1979 Mar;43(1):73–102. doi: 10.1128/mr.43.1.73-102.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Iglewski B. H., Burns R. P., Gipson I. K. Pathogenesis of corneal damage from pseudomonas exotoxin A. Invest Ophthalmol Vis Sci. 1977 Jan;16(1):73–76. [PubMed] [Google Scholar]
  7. Kawaharajo K., Abe C., Homma J. Y., Kawano M., Goto E. Corneal ulcers caused by protease and elastase from Pseudomonas aeruginosa. Jpn J Exp Med. 1974 Oct;44(5):435–442. [PubMed] [Google Scholar]
  8. Kreger A. S., Gray L. D. Purification of Pseudomonas aeruginosa proteases and microscopic characterization of pseudomonal protease-induced rabbit corneal damage. Infect Immun. 1978 Feb;19(2):630–648. doi: 10.1128/iai.19.2.630-648.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ohman D. E., Burns R. P., Iglewski B. H. Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa. J Infect Dis. 1980 Oct;142(4):547–555. doi: 10.1093/infdis/142.4.547. [DOI] [PubMed] [Google Scholar]
  10. Ohman D. E., Sadoff J. C., Iglewski B. H. Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization. Infect Immun. 1980 Jun;28(3):899–908. doi: 10.1128/iai.28.3.899-908.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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