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. 1982 Feb;35(2):533–536. doi: 10.1128/iai.35.2.533-536.1982

Spontaneous production of gamma interferon and induced production of beta interferon by human T-lymphoblastoid cell lines.

A G Morris, J Morser, A Meager
PMCID: PMC351073  PMID: 6173325

Abstract

Two human T-lymphoblastoid cell lines, CCRF/CEM and Molt 4, produced beta interferon (IFN-beta) upon infection with Sendai virus. Molt 4, but not CCRF/CEM, spontaneously produced up to 300 U of IFN-gamma per ml, apparently not contaminated with IFN-alpha or -beta. Phytohemagglutinin, a T-cell mitogen, did not stimulate IFN production in these lines. A third T-lymphoblastoid line, CCRF/HSB2, produced no IFN either spontaneously or after infection with Sendai virus or treatment with phytohemagglutinin. The Molt 4 cells contained an mRNA which could be translated by oocytes to give IFN-gamma. Molt 4 cells therefore provide a convenient source of human IFN-gamma and its mRNA for experimental purposes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins G. J., Johnston M. D., Westmacott L. M., Burke D. C. Department of Biological Sciences, University of Warwick, Coventry, CV47AL, England. J Gen Virol. 1974 Dec;25(3):381–390. doi: 10.1099/0022-1317-25-3-381. [DOI] [PubMed] [Google Scholar]
  2. Attallah A. M., Fleisher T., Khalil R., Noguchi P. D., Urritia-Shaw A. Proliferative and functional aspects of interferon-treated human normal and neoplastic T and B cells. Br J Cancer. 1980 Sep;42(3):423–429. doi: 10.1038/bjc.1980.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colman A., Morser J. Export of proteins from oocytes of Xenopus laevis. Cell. 1979 Jul;17(3):517–526. doi: 10.1016/0092-8674(79)90260-5. [DOI] [PubMed] [Google Scholar]
  4. FOLEY G. E., LAZARUS H., FARBER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  5. Havell E. A., Yip Y. K., Vilcek J. Characteristics of human lymphoblastoid (Namalva) interferon. J Gen Virol. 1978 Jan;38(1):51–59. doi: 10.1099/0022-1317-38-1-51. [DOI] [PubMed] [Google Scholar]
  6. Hovanessian A. G., Meurs E., Aujean O., Vaquero C., Stefanos S., Falcoff E. Antiviral response and induction of specific proteins in cells treated with immune T (type II) interferon analogous to that from viral interferon (type I)-treated cells. Virology. 1980 Jul 15;104(1):195–204. doi: 10.1016/0042-6822(80)90377-3. [DOI] [PubMed] [Google Scholar]
  7. Langford M. P., Georgiades J. A., Stanton G. J., Dianzani F., Johnson H. M. Large-scale production and physicochemical characterization of human immune interferon. Infect Immun. 1979 Oct;26(1):36–41. doi: 10.1128/iai.26.1.36-41.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Larsson I., Lundgren E., Nilsson K., Strannegard O. A human neoplastic hematopoietic cell line producing a fibroblast type of interferon. Dev Biol Stand. 1979;42:193–197. [PubMed] [Google Scholar]
  9. Minowada J., Onuma T., Moore G. E. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst. 1972 Sep;49(3):891–895. [PubMed] [Google Scholar]
  10. Mizrahi A., O'Malley J. A., Carter W. A., Takatsuki A., Tamura G., Sulkowski E. Glycosylation of interferons. Effects of tunicamycin on human immune interferon. J Biol Chem. 1978 Nov 10;253(21):7612–7615. [PubMed] [Google Scholar]
  11. Morser J., Flint J., Meager A., Graves H., Baker P. N., Colman A., Burke D. C. Characterization of interferon messenger RNA from human lymphoblastoid cells. J Gen Virol. 1979 Jul;44(1):231–234. doi: 10.1099/0022-1317-44-1-231. [DOI] [PubMed] [Google Scholar]
  12. Morser J., Meager A., Colman A. Enhancement of interferon mRNA levels in butyric acid-treated Namalwa cells. FEBS Lett. 1980 Apr 7;112(2):203–206. doi: 10.1016/0014-5793(80)80180-3. [DOI] [PubMed] [Google Scholar]
  13. Ponzio N. M., Fitzgerald K. L., Vilcek J., Thorbecke G. J. Spontaneous production of T (type II) interferon by a murine reticulum-cell sarcoma. Ann N Y Acad Sci. 1980;350:157–167. doi: 10.1111/j.1749-6632.1980.tb20616.x. [DOI] [PubMed] [Google Scholar]
  14. Tovey M. G., Begon-Lours J., Gresser I., Morris A. G. Marked enhancement of interferon production in 5-bromodeoxyuridine treated human lymphoblastoid cells. Nature. 1977 Jun 2;267(5610):455–457. doi: 10.1038/267455a0. [DOI] [PubMed] [Google Scholar]
  15. Wietzerbin J., Stefanos S., Lucero M., Falcoff E., O'Malley J. A., Sulkowski E. Physico-chemical characterization and partial purification of mouse immune interferon. J Gen Virol. 1979 Sep;44(3):773–781. doi: 10.1099/0022-1317-44-3-773. [DOI] [PubMed] [Google Scholar]
  16. Wiranowska-Stewart M., Lin L. S., Braude I. A., Stewart W. E., 2nd Production, partial purification and characterization of human and murine interferons--type II. Mol Immunol. 1980 May;17(5):625–633. doi: 10.1016/0161-5890(80)90160-1. [DOI] [PubMed] [Google Scholar]
  17. de Ley M., van Damme J., Claeys H., Weening H., Heine J. W., Billiau A., Vermylen C., de Somer P. Interferon induced in human leukocytes by mitogens: production, partial purification and characterization. Eur J Immunol. 1980 Nov;10(11):877–883. doi: 10.1002/eji.1830101113. [DOI] [PubMed] [Google Scholar]

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