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. 1982 Feb;35(2):560–565. doi: 10.1128/iai.35.2.560-565.1982

Enhanced adoptive transfer of immunity to Listeria monocytogenes after in vitro culture of murine spleen cells with concanavalin A.

R A Barry, D J Hinrichs
PMCID: PMC351077  PMID: 6799407

Abstract

In vitro incubation of spleen cells with T-cell mitogens has been shown to augment cytotoxic and cytolytic T-cell function. The plant lectins Concanavalin A and phytohemagglutinin were employed in a similar fashion to investigate their abilities to enhance cell-mediated immunity to the microbial pathogen Listeria monocytogenes. Spleen cells from immune mice were incubated in vitro with Concanavalin A or phytohemagglutinin before passive transfer into normal recipients. Results indicated a 10- to 100-fold enhancement in the ability of these cells stimulated in vitro to transfer antilisterial resistance, as assayed by changes in 50% lethal dose values and enumeration of splenic bacterial proliferation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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