Abstract
Germfree Osborne-Mendel rats were monoassociated with Actinomyces viscosus or Streptococcus mutans. The adherence and subsequent growth of these organisms on the tooth surface was studied by means of total viable cell counts. Both A. viscosus and S. mutans showed a lag phase and an exponential growth phase, similar to logarithmic growth in batch cultures. The exponential growth rates of S. mutans and A. viscosus were 0.63 h-1 (doubling time [td] = 1.1 h) and 0.24 h-1 (td = 2.9 h), respectively. After a period of rapid growth, the rate declined and the populations approached a steady state. The presence of a sucrose-containing diet did not significantly influence the exponential growth rates of A. viscosus and S. mutans, but had a slight negative effect on the initial adherence of S. mutans at the tooth surface.
Full text
PDF![583](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d11d/351080/50c194b3207e/iai00154-0209.png)
![584](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d11d/351080/0e82209c21fc/iai00154-0210.png)
![585](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d11d/351080/53889eb51409/iai00154-0211.png)
![586](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d11d/351080/b467024e472d/iai00154-0212.png)
![587](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d11d/351080/ce1a343c0b9a/iai00154-0213.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brecher S. M., van Houte J. Relationship between host age and susceptibility to oral colonization by Actinomyces viscosus in Sprague-Dawley rats. Infect Immun. 1979 Dec;26(3):1137–1145. doi: 10.1128/iai.26.3.1137-1145.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brock T. D. Microbial growth rates in nature. Bacteriol Rev. 1971 Mar;35(1):39–58. doi: 10.1128/br.35.1.39-58.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARLSSON J., EGELBERG J. EFFECT OF DIET ON EARLY PLAQUE FORMATION IN MAN. Odontol Revy. 1965;16:112–125. [PubMed] [Google Scholar]
- Clark W. B., Gibbons R. J. Influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of Streptococcus mutans 6715 to hydroxyapatite surfaces. Infect Immun. 1977 Nov;18(2):514–523. doi: 10.1128/iai.18.2.514-523.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cowman R. A., Schaefer S. J., Fitzgerald R. J., Rosner D., Shklair I. L., Walter R. G. Differential utilization of proteins in saliva from caries-active and caries-free subjects as growth substrates by plaque-forming streptococci. J Dent Res. 1979 Oct;58(10):2019–2027. doi: 10.1177/00220345790580101501. [DOI] [PubMed] [Google Scholar]
- Ellen R. P. Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity. Infect Immun. 1976 Nov;14(5):1119–1124. doi: 10.1128/iai.14.5.1119-1124.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIBBONS R. J. BACTERIOLOGY OF DENTAL CARIES. J Dent Res. 1964 Nov-Dec;43:SUPPL–SUPPL:1028. doi: 10.1177/00220345640430060301. [DOI] [PubMed] [Google Scholar]
- Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
- Hamada S., Slade H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980 Jun;44(2):331–384. doi: 10.1128/mr.44.2.331-384.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harder W., Kuenen J. G. A review. Microbial selection in continuous culture. J Appl Bacteriol. 1977 Aug;43(1):1–24. doi: 10.1111/j.1365-2672.1977.tb00717.x. [DOI] [PubMed] [Google Scholar]
- KRASSE B. The proportional distribution of Streptococcus salivarius and other streptococci in various parts of the mouth. Odontol Revy. 1954;5(3):203–211. [PubMed] [Google Scholar]
- Krasse B., Edwardsson S., Svensson I., Trell L. Implantation of caries-inducing streptococci in the human oral cavity. Arch Oral Biol. 1967 Feb;12(2):231–236. doi: 10.1016/0003-9969(67)90042-8. [DOI] [PubMed] [Google Scholar]
- Kraus F. W., Orstavik D., Hurst D. C., Cook C. H. The acquired pellicle: variability and subject-dependence of specific proteins. J Oral Pathol. 1973;2(3):165–173. doi: 10.1111/j.1600-0714.1973.tb01680.x. [DOI] [PubMed] [Google Scholar]
- Olson G. A., Guggenheim B., Small P. A., Jr Antibody-mediated inhibition of dextran-sucrose-induced agglutination of Streptococcus mutans. Infect Immun. 1974 Feb;9(2):273–278. doi: 10.1128/iai.9.2.273-278.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogers A. H. Bacteriocinogeny and the properties of some bacteriocins of Streptococcus mutans. Arch Oral Biol. 1976;21(2):99–104. doi: 10.1016/0003-9969(76)90079-0. [DOI] [PubMed] [Google Scholar]
- Slee A. M., Tanzer J. M. Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in five serotypes of Streptococcus mutans. Infect Immun. 1979 Nov;26(2):783–786. doi: 10.1128/iai.26.2.783-786.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Socransky S. S., Manganiello A. D., Propas D., Oram V., van Houte J. Bacteriological studies of developing supragingival dental plaque. J Periodontal Res. 1977 Mar;12(2):90–106. doi: 10.1111/j.1600-0765.1977.tb00112.x. [DOI] [PubMed] [Google Scholar]
- St Martin E. J., Wittenberger C. L. Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans. Infect Immun. 1979 Jun;24(3):865–868. doi: 10.1128/iai.24.3.865-868.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Houte J., Gibbons R. J., Pulkkinen A. J. Adherence as an ecological determinant for streptococci in the human mouth. Arch Oral Biol. 1971 Oct;16(10):1131–1141. doi: 10.1016/0003-9969(71)90042-2. [DOI] [PubMed] [Google Scholar]
- Van Houte J., Upeslacis V. N., Jordan H. V., Skobe Z., Green D. B. Role of sucrose in colonization of Streptococcus mutans in conventional Sprague-Dawley rats. J Dent Res. 1976 Mar-Apr;55(2):202–215. doi: 10.1177/00220345760550020801. [DOI] [PubMed] [Google Scholar]
- Zobell C. E. The Effect of Solid Surfaces upon Bacterial Activity. J Bacteriol. 1943 Jul;46(1):39–56. doi: 10.1128/jb.46.1.39-56.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Houte J., Winkler K. C., Jansen H. M. Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci. Arch Oral Biol. 1969 Jan;14(1):45–61. doi: 10.1016/0003-9969(69)90020-x. [DOI] [PubMed] [Google Scholar]
- van der Hoeven J. S. A slime-producing microorganism in dental plaque of rats, selected by glucose feeding. Chemical composition of extracellular slime elaborated by Actinomyces viscosus, strain Nyl. Caries Res. 1974;8(3):193–210. doi: 10.1159/000260109. [DOI] [PubMed] [Google Scholar]