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. 1982 Feb;35(2):605–611. doi: 10.1128/iai.35.2.605-611.1982

Serum resistance associated with virulence in Yersinia enterocolitica.

C H Pai, L DeStephano
PMCID: PMC351084  PMID: 7056577

Abstract

Yersinia enterocolitica strains that exhibited a calcium requirement for growth and autoagglutination at 37 degrees C were invariably virulent in rabbits, causing diarrhea and a high degree of lethality, and were capable of colonizing the intestinal lumen and establishing foci of infection on the Peyer's patches of mice. Strains that had lost the properties of calcium dependency and autoagglutinability were totally avirulent in rabbits and were quickly eliminated from the intestinal lumen and tissues of mice. Virulent and avirulent strains were shown to be equally invasive to HeLa cells. However, the virulent strains were resistant to the bactericidal action of normal serum, and this serum resistance was lost with the loss of virulence. Furthermore, the serum resistance of virulent strains was expressed, as were other properties, when strains were grown at 37 degrees C, but not at 27 degrees C. These results suggest that a virulence factor associated with serum resistance plays an essential role in the pathogenicity of Y. enterocolitica.

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Selected References

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  1. Alonso J. M., Bercovier H., Destombes P., Mollaret H. H. Pouvoir pathogène expérimental de Yersinia enterocolitica chez la souris athymique (nude) Ann Microbiol (Paris) 1975 Sep;126(2):187–199. [PubMed] [Google Scholar]
  2. BACON G. A., BURROWS T. W. The basis of virulence in Pasteurella pestis: an antigen determining virulence. Br J Exp Pathol. 1956 Oct;37(5):481–493. [PMC free article] [PubMed] [Google Scholar]
  3. BRUBAKER R. R., SURGALLA M. J. THE EFFECT OF CA++ AND MG++ ON LYSIS, GROWTH, AND PRODUCTION OF VIRULENCE ANTIGENS BY PASTEURELLA PESTIS. J Infect Dis. 1964 Feb;114:13–25. doi: 10.1093/infdis/114.1.13. [DOI] [PubMed] [Google Scholar]
  4. Binns M. M., Davies D. L., Hardy K. G. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature. 1979 Jun 28;279(5716):778–781. doi: 10.1038/279778a0. [DOI] [PubMed] [Google Scholar]
  5. Bottone E. J. Yersinia enterocolitica: a panoramic view of a charismatic microorganism. CRC Crit Rev Microbiol. 1977;5(2):211–241. doi: 10.3109/10408417709102312. [DOI] [PubMed] [Google Scholar]
  6. Bradford W. D., Noce P. S., Gutman L. T. Pathologic features of enteric infection with Yersinia enterocolitica. Arch Pathol. 1974 Jul;98(1):17–22. [PubMed] [Google Scholar]
  7. Brubaker R. R. The genus Yersinia: biochemistry and genetics of virulence. Curr Top Microbiol Immunol. 1972;57:111–158. doi: 10.1007/978-3-642-65297-4_4. [DOI] [PubMed] [Google Scholar]
  8. Carter P. B. Pathogenecity of Yersinia enterocolitica for mice. Infect Immun. 1975 Jan;11(1):164–170. doi: 10.1128/iai.11.1.164-170.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Carter P. B., Zahorchak R. J., Brubaker R. R. Plague virulence antigens from Yersinia enterocolitica. Infect Immun. 1980 May;28(2):638–640. doi: 10.1128/iai.28.2.638-640.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feeley J. C., Wells J. G., Tsai T. F., Puhr N. D. Detection of enterotoxigenic and invasive strains of Yersinia enterocolitica. Contrib Microbiol Immunol. 1979;5:329–334. [PubMed] [Google Scholar]
  11. Ferber D. M., Brubaker R. R. Plasmids in Yersinia pestis. Infect Immun. 1981 Feb;31(2):839–841. doi: 10.1128/iai.31.2.839-841.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gemski P., Lazere J. R., Casey T. Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980 Feb;27(2):682–685. doi: 10.1128/iai.27.2.682-685.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gemski P., Lazere J. R., Casey T., Wohlhieter J. A. Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infect Immun. 1980 Jun;28(3):1044–1047. doi: 10.1128/iai.28.3.1044-1047.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kohl S., Jacobson J. A., Nahmias A. Yersinia enterocolitica infections in children. J Pediatr. 1976 Jul;89(1):77–79. doi: 10.1016/s0022-3476(76)80932-8. [DOI] [PubMed] [Google Scholar]
  15. LAWTON W. D., ERDMAN R. L., SURGALLA M. J. BIOSYNTHESIS AND PURIFICATION OF V AND W ANTIGEN IN PASTEURELLA PESTIS. J Immunol. 1963 Aug;91:179–184. doi: 10.21236/ad0299868. [DOI] [PubMed] [Google Scholar]
  16. Laird W. J., Cavanaugh D. C. Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980 Apr;11(4):430–432. doi: 10.1128/jcm.11.4.430-432.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
  18. Marks M. I., Pai C. H., Lafleur L., Lackman L., Hammerberg O. Yersinia enterocolitica gastroenteritis: a prospective study of clinical, bacteriologic, and epidemiologic features. J Pediatr. 1980 Jan;96(1):26–31. doi: 10.1016/s0022-3476(80)80318-0. [DOI] [PubMed] [Google Scholar]
  19. Mehlman I. J., Eide E. L., Sanders A. C., Fishbein M., Aulisio C. C. Methodology for recognition of invasive potential of Escherichia coli. J Assoc Off Anal Chem. 1977 May;60(3):546–562. [PubMed] [Google Scholar]
  20. Mors V., Pai C. H. Pathogenic properties of Yersinia enterocolitica. Infect Immun. 1980 Apr;28(1):292–294. doi: 10.1128/iai.28.1.292-294.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pai C. H., Mors V. Production of enterotoxin by Yersinia enterocolitica. Infect Immun. 1978 Mar;19(3):908–911. doi: 10.1128/iai.19.3.908-911.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pai C. H., Mors V., Seemayer T. A. Experimental Yersinia enterocolitica enteritis in rabbits. Infect Immun. 1980 Apr;28(1):238–244. doi: 10.1128/iai.28.1.238-244.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pai C. H., Mors V., Toma S. Prevalence of enterotoxigenicity in human and nonhuman isolates of Yersinia enterocolitica. Infect Immun. 1978 Nov;22(2):334–338. doi: 10.1128/iai.22.2.334-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pearson A. D., Ricciardi I. D., Wright D. H., Suckling W. G. An experimental study of the pathology and ecology of Yersinia enterocolitica infection in mice. Contrib Microbiol Immunol. 1979;5:335–345. [PubMed] [Google Scholar]
  25. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
  27. Taylor P. W., Parton R. A protein factor associated with serum resistance in Escherichia coli. J Med Microbiol. 1977 May;10(2):225–232. doi: 10.1099/00222615-10-2-225. [DOI] [PubMed] [Google Scholar]
  28. Une T. Studies on the pathogenicity of Yersinia enterocolitica. I. Experimental infection in rabbits. Microbiol Immunol. 1977;21(7):341–363. [PubMed] [Google Scholar]
  29. Une T. Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro. Microbiol Immunol. 1977;21(7):365–377. doi: 10.1111/j.1348-0421.1977.tb00301.x. [DOI] [PubMed] [Google Scholar]
  30. Zink D. L., Feeley J. C., Wells J. G., Vanderzant C., Vickery J. C., Roof W. D., O'Donovan G. A. Plasmid-mediated tissue invasiveness in Yersinia enterocolitica. Nature. 1980 Jan 10;283(5743):224–226. doi: 10.1038/283224a0. [DOI] [PubMed] [Google Scholar]

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