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. 1982 Feb;35(2):627–632. doi: 10.1128/iai.35.2.627-632.1982

Plasmid pattern analysis of Staphylococcal epidermidis isolates from patients with prosthetic valve endocarditis.

G L Archer, N Vishniavsky, H G Stiver
PMCID: PMC351087  PMID: 7056579

Abstract

The electrophoretic pattern formed by individual bacterial plasmid DNA molecules of differing molecular size was evaluated as an epidemiological marker among isolates of Staphylococcus epidermidis from patients with prosthetic valve endocarditis (PVE). Purified covalently closed circular plasmid DNA was obtained from selected isolates, and 79% of the plasmids were found to be less than 10 megadaltons in size; only these small plasmids were sought in subsequent screening gels. Crude cell lysates obtained by a rapid lysis technique and screened by agarose gel electrophoresis revealed the presence of one or more small plasmids in 54 of 58 (93%) PVE isolates; 79% contained two or more. Among 45 plasmid-containing isolates from cases of sporadic PVE at three institutions there were no identical plasmid patterns, although several isolates differed by a single plasmid. In contrast, among nine isolates from a cluster of cases of PVE in Canada, two groups of three isolates each had identical plasmid patterns. Additional clinical data suggested that these isolates were epidemiologically related. Phage typing distinguished one of the groups with plasmid pattern identity, but not the other, from the three isolates with dissimilar patterns. Plasmid pattern analysis shows promise as an epidemiological marker for clinically important isolates of S. epidermidis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archer G. L. Antimicrobial susceptibility and selection of resistance among Staphylococcus epidermidis isolates recovered from patients with infections of indwelling foreign devices. Antimicrob Agents Chemother. 1978 Sep;14(3):353–359. doi: 10.1128/aac.14.3.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Archer G. L., Tenenbaum M. J. Antibiotic-resistant Staphylococcus epidermidis in patients undergoing cardiac surgery. Antimicrob Agents Chemother. 1980 Feb;17(2):269–272. doi: 10.1128/aac.17.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  4. Blouse L., Kolonel L. N., Watkins C. A., Atherton J. M. Efficacy of phage typing epidemiologically related Staphylococcus epidermidis strains. J Clin Microbiol. 1975 Oct;2(4):318–321. doi: 10.1128/jcm.2.4.318-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hammond G. W., Stiver H. G. Combination antibiotic therapy in an outbreak of prosthetic endocarditis caused by Staphylococcus epidermidis. Can Med Assoc J. 1978 Mar 4;118(5):524–530. [PMC free article] [PubMed] [Google Scholar]
  6. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  7. Macrina F. L., Reider J. L., Virgili S. S., Kopecko D. J. Survey of the extrachromosomal gene pool of Streptococcus mutans. Infect Immun. 1977 Jul;17(1):215–226. doi: 10.1128/iai.17.1.215-226.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Macrina F. L., Wood P. H., Jones K. R. Simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci. Appl Environ Microbiol. 1980 May;39(5):1070–1073. doi: 10.1128/aem.39.5.1070-1073.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Masur H., Johnson W. D., Jr Prosthetic valve endocarditis. J Thorac Cardiovasc Surg. 1980 Jul;80(1):31–37. [PubMed] [Google Scholar]
  10. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Parisi J. T., Hecht D. W. Plasmid profiles in epidemiologic studies of infections by Staphylococcus epidermidis. J Infect Dis. 1980 May;141(5):637–643. doi: 10.1093/infdis/141.5.637. [DOI] [PubMed] [Google Scholar]
  12. Richardson J. V., Karp R. B., Kirklin J. W., Dismukes W. E. Treatment of infective endocarditis: a 10-year comparative analysis. Circulation. 1978 Oct;58(4):589–597. doi: 10.1161/01.cir.58.4.589. [DOI] [PubMed] [Google Scholar]
  13. Rubens C. E., Farrar W. E., Jr, McGee Z. A., Schaffner W. Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. J Infect Dis. 1981 Feb;143(2):170–181. doi: 10.1093/infdis/143.2.170. [DOI] [PubMed] [Google Scholar]
  14. Schaberg D. R., Tompkins L. S., Falkow S. Use of agarose gel electrophoresis of plasmid deoxyribonucleic acid to fingerprint gram-negative bacilli. J Clin Microbiol. 1981 Jun;13(6):1105–1108. doi: 10.1128/jcm.13.6.1105-1108.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Skahan J. M., Parisi J. T. Development of a bacteriophage-typing set for Staphylococcus epidermidis. J Clin Microbiol. 1977 Jul;6(1):16–18. doi: 10.1128/jcm.6.1.16-18.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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