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. 1982 Mar;35(3):764–769. doi: 10.1128/iai.35.3.764-769.1982

Identification of a new genetic site (sac-3+) in Neisseria gonorrhoeae that affects sensitivity to normal human serum.

W M Shafer, L F Guymon, P F Sparling
PMCID: PMC351114  PMID: 6802757

Abstract

A previously undescribed genetic site (sac-3) affecting susceptibility of the gonococcus to normal human serum was localized on the gonococcal chromosome. The presence of the sac-3+ allele in a clinical isolate (FA889) resulted in sensitivity only to relatively high concentrations of serum (greater than or equal to 12.5%). Genetic mapping experiments demonstrated that sac-3+ was tightly linked to another genetic site (sac-1+) involved in determining susceptibility to normal human serum and to a locus (nmp-3) involved in the replacement of outer membrane protein I. The sac-1+ and sac-3+ loci resulted in phenotypically distinct levels of sensitivity to human serum. The sac-3+ serum sensitivity and sac-1+ serum sensitivity loci recombined with high frequency, resulting in serum resistance. The results show that serum sensitivity in clinical isolates may be due to different serum sensitivity loci and suggest that different antigens and immunological mechanisms could be responsible for sensitivity of different gonococcal isolates to human serum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brooks G. F., Ingwer I. Studies on the relationships between serum bactericidal activity and uncomplicated genital infections due to Neisseria gonorrhoeae. J Infect Dis. 1978 Sep;138(3):333–339. doi: 10.1093/infdis/138.3.333. [DOI] [PubMed] [Google Scholar]
  2. Cannon J. G., Klapper D. G., Blackman E. Y., Sparling P. F. Genetic locus (nmp-1) affecting the principal outer membrane protein of Neisseria gonorrhoeae. J Bacteriol. 1980 Aug;143(2):847–851. doi: 10.1128/jb.143.2.847-851.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannon J. G., Lee T. J., Guymon L. F., Sparling P. F. Genetics of serum resistance in Neisseria gonorrhoeae: the sac-1 genetic locus. Infect Immun. 1981 May;32(2):547–552. doi: 10.1128/iai.32.2.547-552.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W. Genetic transformation of biosynthetically defective Neisseria gonorrhoeae clinical isolates. J Bacteriol. 1974 Oct;120(1):203–209. doi: 10.1128/jb.120.1.203-209.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eisenstein B. I., Lee T. J., Sparling P. F. Penicillin sensitivity and serum resistance are independent attributes of strains of Neisseria gonorrhoeae causing disseminated gonococcal infection. Infect Immun. 1977 Mar;15(3):834–841. doi: 10.1128/iai.15.3.834-841.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heckels J. E. Structural comparison of Neisseria gonorrhoeae outer membrane proteins. J Bacteriol. 1981 Feb;145(2):736–742. doi: 10.1128/jb.145.2.736-742.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hildebrandt J. F., Mayer L. W., Wang S. P., Buchanan T. M. Neisseria gonorrhoeae acquire a new principal outer-membrane protein when transformed to resistance to serum bactericidal activity. Infect Immun. 1978 Apr;20(1):267–272. doi: 10.1128/iai.20.1.267-272.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  9. Lambden P. R., Heckels J. E., James L. T., Watt P. J. Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. J Gen Microbiol. 1979 Oct;114(2):305–312. doi: 10.1099/00221287-114-2-305. [DOI] [PubMed] [Google Scholar]
  10. Lambden P. R., Robertson J. N., Watt P. J. Biological properties of two distinct pilus types produced by isogenic variants of Neisseria gonorrhoeae P9. J Bacteriol. 1980 Jan;141(1):393–396. doi: 10.1128/jb.141.1.393-396.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mayer L. W., Schoolnik G. K., Falkow S. Genetic studies on Neisseria gonorrhoeae from disseminated gonococcal infections. Infect Immun. 1977 Oct;18(1):165–172. doi: 10.1128/iai.18.1.165-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schoolnik G. K., Buchanan T. M., Holmes K. K. Gonococci causing disseminated gonococcal infection are resistant to the bactericidal action of normal human sera. J Clin Invest. 1976 Nov;58(5):1163–1173. doi: 10.1172/JCI108569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  14. Sparling P. F., Sarubbi F. A., Jr, Blackman E. Inheritance of low-level resistance to penicillin, tetracycline, and chloramphenicol in Neisseria gonorrhoeae. J Bacteriol. 1975 Nov;124(2):740–749. doi: 10.1128/jb.124.2.740-749.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spratt S. K., Jones F., Shockley T. E., Jackson J. H. Cotransformation of a serum resistance phenotype with genes for arginine biosynthesis in Neisseria gonorrhoeae. Infect Immun. 1980 Jul;29(1):287–289. doi: 10.1128/iai.29.1.287-289.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Swanson J. Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci. Infect Immun. 1978 Jan;19(1):320–331. doi: 10.1128/iai.19.1.320-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Walstad D. L., Guymon L. F., Sparling P. F. Altered outer membrane protein in different colonial types of Neisseria gonorrhoeae. J Bacteriol. 1977 Mar;129(3):1623–1627. doi: 10.1128/jb.129.3.1623-1627.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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