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. 1982 Mar;35(3):909–914. doi: 10.1128/iai.35.3.909-914.1982

Cell-mediated immunity in malnourished guinea pigs after Mycobacterium bovis BCG vaccination.

D N McMurray, E A Yetley
PMCID: PMC351133  PMID: 7040251

Abstract

Specific-pathogen-free guinea pigs were vaccinated with viable Mycobacterium bovis BCG and maintained on purified, isocaloric diets containing either 30% or 7.5% casein, or commercial chow. At intervals of 4, 5, 6, and 8 weeks postvaccination, groups of guinea pigs from each diet treatment were skin tested with purified protein derivative and killed. Protein-deficient animals exhibited progressive reductions in total serum proteins and albumin. Significantly greater numbers of viable M. bovis BCG were recovered from the vaccination site and inguinal lymph nodes of protein-deficient guinea pigs at all intervals. In contrast, the development of delayed hypersensitivity was markedly retarded in the 7.5% casein group and was also reduced somewhat in the 30% casein group as compared to chow control. Peripheral blood lymphocytes from protein-deficient animals did not respond normally in vitro to a polyclonal T cell mitogen, phytohemagglutinin. These results demonstrate that protein-calorie malnutrition in this model impairs the development of cell-mediated immunity as evidenced by skin test anergy, lymphocyte hyporesponsiveness, and failure to control levels of viable M. bovis BCG after vaccination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbassy A. S., el-Din M. K., Hassan A. I., Aref G. H., Hammad S. A., el-Araby I. I., el-Din A. A., Soliman M. H., Hussein M. Studies of cell-mediated immunity and allergy in protein energy malnutrition. I. Cell-mediated delayed hypersensitivity. J Trop Med Hyg. 1974 Jan;77(1):13–17. [PubMed] [Google Scholar]
  2. Beisel W. R., Edelman R., Nauss K., Suskind R. M. Single-nutrient effects on immunologic functions. Report of a workshop sponsored by the Department of Food and Nutrition and its nutrition advisory group of the American Medical Association. JAMA. 1981 Jan 2;245(1):53–58. doi: 10.1001/jama.245.1.53. [DOI] [PubMed] [Google Scholar]
  3. Bhuyan U. N., Ramalingaswami V. Immune responses of the protein-deficient guinea pig to BCG vaccination. Am J Pathol. 1973 Sep;72(3):489–502. [PMC free article] [PubMed] [Google Scholar]
  4. Bhuyan U. N., Ramalingaswami V. Systemic macrophage mobilization and granulomatous response to BCG in the protein-deficient rabbit. Am J Pathol. 1974 Aug;76(2):313–322. [PMC free article] [PubMed] [Google Scholar]
  5. Chandra R. K., Au B. Single nutrient deficiency and cell-mediated immune responses. I. Zinc. Am J Clin Nutr. 1980 Apr;33(4):736–738. doi: 10.1093/ajcn/33.4.736. [DOI] [PubMed] [Google Scholar]
  6. Chandra R. K. Cell-mediated immunity in nutritional imbalance. Fed Proc. 1980 Nov;39(13):3088–3092. [PubMed] [Google Scholar]
  7. Collins F. M., Auclair L. Lymphatic drainage in BCG-infected guinea pigs. J Reticuloendothel Soc. 1977 Jul;22(1):35–44. [PubMed] [Google Scholar]
  8. Good R. A., Fernandes G., Yunis E. J., Cooper W. C., Jose D. C., Kramer T. R., Hansen M. A. Nutritional deficiency, immunologic function, and disease. Am J Pathol. 1976 Sep;84(3):599–614. [PMC free article] [PubMed] [Google Scholar]
  9. Good R. A., West A., Fernandes G. Nutritional modulation of immune responses. Fed Proc. 1980 Nov;39(13):3098–3104. [PubMed] [Google Scholar]
  10. HARLAND P. S., BROWN R. E. TUBERCULIN SENSITIVITY FOLLOWING B.C.G. VACCINATION IN UNDERNOURISHED CHILDREN. East Afr Med J. 1965 May;42:233–238. [PubMed] [Google Scholar]
  11. Han T., Pauly J. Simplified whole blood method for evaluating in vitro lymphocyte reactivity of laboratory animals. Clin Exp Immunol. 1972 May;11(1):137–142. [PMC free article] [PubMed] [Google Scholar]
  12. Harland P. S. Tuberculin reactions in malnourished children. Lancet. 1965 Oct 9;2(7415):719–721. doi: 10.1016/s0140-6736(65)90457-5. [DOI] [PubMed] [Google Scholar]
  13. Heresi G. P., Saitúa M. T., Schlesinger L. Leukocyte migration inhibition factor production in marasmic infants. Am J Clin Nutr. 1981 May;34(5):909–913. doi: 10.1093/ajcn/34.5.909. [DOI] [PubMed] [Google Scholar]
  14. Heresi G., Chandra R. K. Effects of severe calorie restriction on thymic factor activity and lymphocyte stimulation response in rats. J Nutr. 1980 Sep;110(9):1888–1893. doi: 10.1093/jn/110.9.1888. [DOI] [PubMed] [Google Scholar]
  15. Koster F., Gaffar A., Jackson T. M. Recovery of cellular immune competence during treatment of protein-calorie malnutrition. Am J Clin Nutr. 1981 May;34(5):887–891. doi: 10.1093/ajcn/34.5.887. [DOI] [PubMed] [Google Scholar]
  16. Kramer T. R., Good R. A. Increased in vitro cell-mediated immunity in protein-malnourished guinea pigs. Clin Immunol Immunopathol. 1978 Oct;11(2):212–228. doi: 10.1016/0090-1229(78)90045-4. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Ladefoged A., Bunch-Christensen K., Guld J. Tuberculin sensitivity in guinea-pigs after vaccination with varying doses of BCG of 12 different strains. Bull World Health Organ. 1976;53(4):435–443. [PMC free article] [PubMed] [Google Scholar]
  19. McMurray D. N. Cellular immune changes in undernourished children. Prog Clin Biol Res. 1981;67:305–318. [PubMed] [Google Scholar]
  20. McMurray D. N., Loomis S. A., Casazza L. J., Rey H., Miranda R. Development of impaired cell-mediated immunity in mild and moderate malnutrition. Am J Clin Nutr. 1981 Jan;34(1):68–77. doi: 10.1093/ajcn/34.1.68. [DOI] [PubMed] [Google Scholar]
  21. McMurray D. N., Watson R. R., Reyes M. A. Effect of renutrition on humoral and cell-mediated immunity in severely malnourished children. Am J Clin Nutr. 1981 Oct;34(10):2117–2126. doi: 10.1093/ajcn/34.10.2117. [DOI] [PubMed] [Google Scholar]
  22. Narain R., Lakshminarayana P., Vallishayee R. S., Narmada R., Diwakara A. M. Influence of nutrition on delayed skin hypersensitivity. Indian J Med Res. 1979 Jan;69:6–17. [PubMed] [Google Scholar]
  23. Neumann C. G., Lawlor G. J., Jr, Stiehm E. R., Swenseid M. E., Newton C., Herbert J., Ammann A. J., Jacob M. Immunologic responses in malnourished children. Am J Clin Nutr. 1975 Feb;28(2):89–104. doi: 10.1093/ajcn/28.2.89. [DOI] [PubMed] [Google Scholar]
  24. Nixon D. W., Heymsfield S. B., Cohen A. E., Kutner M. H., Ansley J., Lawson D. H., Rudman D. Protein-calorie undernutrition in hospitalized cancer patients. Am J Med. 1980 May;68(5):683–690. doi: 10.1016/0002-9343(80)90254-5. [DOI] [PubMed] [Google Scholar]
  25. Satyanarayana K., Bhaskaram P., Seshu V. C., Reddy V. Influence of nutrition on postvaccinial tuberculin sensitivity. Am J Clin Nutr. 1980 Nov;33(11):2334–2337. doi: 10.1093/ajcn/33.11.2334. [DOI] [PubMed] [Google Scholar]
  26. Scrimshaw N. S., Taylor C. E., Gordon J. E. Interactions of nutrition and infection. Monogr Ser World Health Organ. 1968;57:3–329. [PubMed] [Google Scholar]
  27. Shapiro C. D., Harding G. E., Smith D. W. Relationship of delayed-type hypersensitivity and acquired cellular resistance in experimental airborne tuberculosis. J Infect Dis. 1974 Jul;130(1):8–15. doi: 10.1093/infdis/130.1.8. [DOI] [PubMed] [Google Scholar]
  28. Sinha D. P., Bang F. B. Protein and calorie malnutrition, cell-mediated immunity, and B.C.G. vaccination in children from rural West Bengal. Lancet. 1976 Sep 11;2(7985):531–534. doi: 10.1016/s0140-6736(76)91791-8. [DOI] [PubMed] [Google Scholar]
  29. Smythe P. M., Brereton-Stiles G. G., Grace H. J., Mafoyane A., Schonland M., Coovadia H. M., Loening W. E., Parent M. A., Vos G. H. Thymolymphatic deficiency and depression of cell-mediated immunity in protein-calorie malnutrition. Lancet. 1971 Oct 30;2(7731):939–943. doi: 10.1016/s0140-6736(71)90267-4. [DOI] [PubMed] [Google Scholar]
  30. Ziegler H. D., Ziegler P. B. Depression of tuberculin reaction in mild and moderate protein-calorie malnourished children following BCG vaccination. Johns Hopkins Med J. 1975 Aug;137(2):59–64. [PubMed] [Google Scholar]

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