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. 1982 Mar;35(3):915–920. doi: 10.1128/iai.35.3.915-920.1982

Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from lactoferrin.

P A Mickelsen, E Blackman, P F Sparling
PMCID: PMC351134  PMID: 6121757

Abstract

The ability of 107 Neisseria isolates to compete for iron with human lactoferrin (LF) was examined. Each of 15 meningococci, 53% of 59 selected gonococci, and 24% of 33 commensal Neisseria could use LF-bound iron for growth. Isolates which could not obtain iron from LF were growth inhibited when sufficient LF was added to defined agar medium to bind available free iron. No difference was observed in the ability of colony type 1 and colony type 4 gonococci of the same strain to compete with LF for iron. LF was growth inhibitory for 50% of 22 disseminated disease isolates (DGI strains) and 51% of 35 local urogenital disease isolates (UGI strains). Only 14% of gonococcal isolates requiring arginine, hypoxanthine, and uracil for growth were able to compete with LF for iron, whereas 87% of all other gonococcal isolates could do so (P less than 0.005). Ability to obtain iron from LF does not appear to be required for survival of Neisseria on mucosal surfaces, nor essential for invasion of the bloodstream by gonococci. However, ability to utilize LF as a source of iron may contribute to differences in pathogenicity among certain gonococcal isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Archibald F. S., DeVoe I. W. Iron acquisition by Neisseria meningitidis in vitro. Infect Immun. 1980 Feb;27(2):322–334. doi: 10.1128/iai.27.2.322-334.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnold R. R., Brewer M., Gauthier J. J. Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms. Infect Immun. 1980 Jun;28(3):893–898. doi: 10.1128/iai.28.3.893-898.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Arnold R. R., Russell J. E., Champion W. J., Gauthier J. J. Bactericidal activity of human lactoferrin: influence of physical conditions and metabolic state of the target microorganism. Infect Immun. 1981 May;32(2):655–660. doi: 10.1128/iai.32.2.655-660.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bullen J. J., Rogers H. J., Griffiths E. Role of iron in bacterial infection. Curr Top Microbiol Immunol. 1978;80:1–35. doi: 10.1007/978-3-642-66956-9_1. [DOI] [PubMed] [Google Scholar]
  5. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  6. Crawford C., Knapp J. S., Hale J., Holmes K. K. Asymptomatic gonorrhea in men: caused by gonococci with unique nutritional requirements. Science. 1977 Jun 17;196(4296):1352–1353. doi: 10.1126/science.405742. [DOI] [PubMed] [Google Scholar]
  7. Crosa J. H. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature. 1980 Apr 10;284(5756):566–568. doi: 10.1038/284566a0. [DOI] [PubMed] [Google Scholar]
  8. Draper D. L., James J. F., Hadley W. K., Sweet R. L. Auxotypes and antibiotic susceptibilities of Neisseria gonorrhoeae from women with acute salpingitis. Comparison with gonococci causing uncomplicated genital tract infections in women. Sex Transm Dis. 1981 Apr-Jun;8(2):43–50. doi: 10.1097/00007435-198104000-00001. [DOI] [PubMed] [Google Scholar]
  9. Eisenstein B. I., Lee T. J., Sparling P. F. Penicillin sensitivity and serum resistance are independent attributes of strains of Neisseria gonorrhoeae causing disseminated gonococcal infection. Infect Immun. 1977 Mar;15(3):834–841. doi: 10.1128/iai.15.3.834-841.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hebeler B. H., Young F. E. Autolysis of Neisseria gonorrhoeae. J Bacteriol. 1975 May;122(2):385–392. doi: 10.1128/jb.122.2.385-392.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holbein B. E. Iron-controlled infection with Neisseria meningitidis in mice. Infect Immun. 1980 Sep;29(3):886–891. doi: 10.1128/iai.29.3.886-891.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holbein B. E., Jericho K. W., Likes G. C. Neisseria meningitidis infection in mice: influence of iron, variations in virulence among strains, and pathology. Infect Immun. 1979 May;24(2):545–551. doi: 10.1128/iai.24.2.545-551.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holmes K. K., Counts G. W., Beaty H. N. Disseminated gonococcal infection. Ann Intern Med. 1971 Jun;74(6):979–993. doi: 10.7326/0003-4819-74-6-979. [DOI] [PubMed] [Google Scholar]
  14. James J. F., Swanson J. Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun. 1978 Jan;19(1):332–340. doi: 10.1128/iai.19.1.332-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  18. Kojima N., Bates G. W. The reduction and release of iron from Fe3+ .transferrin.CO3(2-). J Biol Chem. 1979 Sep 25;254(18):8847–8854. [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Marcelis J. H., den Daas-Slagt H. J., Hoogkamp-Korstanje J. A. Iron requirement and chelator production of staphylococci, Streptococcus faecalis and enterobacteriaceae. Antonie Van Leeuwenhoek. 1978;44(3-4):257–267. doi: 10.1007/BF00394304. [DOI] [PubMed] [Google Scholar]
  21. Masson P. L., Heremans J. F., Prignot J. J., Wauters G. Immunohistochemical localization and bacteriostatic properties of an iron-binding protein from bronchial mucus. Thorax. 1966 Nov;21(6):538–544. doi: 10.1136/thx.21.6.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Masson P. L., Heremans J. F., Schonne E. Lactoferrin, an iron-binding protein in neutrophilic leukocytes. J Exp Med. 1969 Sep 1;130(3):643–658. doi: 10.1084/jem.130.3.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mickelsen P. A., Sparling P. F. Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infect Immun. 1981 Aug;33(2):555–564. doi: 10.1128/iai.33.2.555-564.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Querinjean P., Masson P. L., Heremans J. F. Molecular weight, single-chain structure and amino acid composition of human lactoferrin. Eur J Biochem. 1971 Jun 11;20(3):420–425. doi: 10.1111/j.1432-1033.1971.tb01408.x. [DOI] [PubMed] [Google Scholar]
  25. Reiter B., Brock J. H., Steel E. D. Inhibition of Escherichia coli by bovine colostrum and post-colostral milk. II. The bacteriostatic effect of lactoferrin on a serum susceptible and serum resistant strain of E. coli. Immunology. 1975 Jan;28(1):83–95. [PMC free article] [PubMed] [Google Scholar]
  26. Salit I. E., Gotschlich E. C. Gonococcal color and opacity variants: virulence for chicken embryos. Infect Immun. 1978 Nov;22(2):359–364. doi: 10.1128/iai.22.2.359-364.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schoolnik G. K., Buchanan T. M., Holmes K. K. Gonococci causing disseminated gonococcal infection are resistant to the bactericidal action of normal human sera. J Clin Invest. 1976 Nov;58(5):1163–1173. doi: 10.1172/JCI108569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sox T. E., Mohammed W., Blackman E., Biswas G., Sparling P. F. Conjugative plasmids in Neisseria gonorrhoeae. J Bacteriol. 1978 Apr;134(1):278–286. doi: 10.1128/jb.134.1.278-286.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Swanson J. Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci. Infect Immun. 1978 Jan;19(1):320–331. doi: 10.1128/iai.19.1.320-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Van Snick J. L., Masson P. L., Heremans J. F. The involvement of lactoferrin in the hyposideremia of acute inflammation. J Exp Med. 1974 Oct 1;140(4):1068–1084. doi: 10.1084/jem.140.4.1068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yancey R. J., Finkelstein R. A. Siderophore production by pathogenic Neisseria spp. Infect Immun. 1981 May;32(2):600–608. doi: 10.1128/iai.32.2.600-608.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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