Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Mar;35(3):1041–1047. doi: 10.1128/iai.35.3.1041-1047.1982

Effect of infectious bovine rhinotracheitis virus infection on bovine alveolar macrophage function.

A J Forman, L A Babiuk
PMCID: PMC351152  PMID: 6279511

Abstract

Bovine alveolar macrophages isolated in culture were assessed for immunological activity in assays for Fc and complement receptors, for phagocytosis, and for effector cell function in antibody-dependent cell cytotoxicity. In the case of uninfected alveolar macrophages, Fc receptors were detected on approximately 94% of macrophages and complement receptors were detected on 39%. Phagocytosis of immunoglobulin G-coated sheep erythrocytes occurred in 58% of macrophages, and phagocytosis of opsonized Candida parapsilosis, mediated by the complement receptor, was observed in 68% of cells. Alveolar macrophages were efficient effector cells in antibody-dependent cell cytotoxicity. Infection of macrophages with infectious bovine rhinotracheitis (IBR) virus resulted in reductions in Fc-mediated receptor activity and phagocytosis after approximately 12 and 6 h, respectively. Complement receptor activity was initially elevated and then markedly reduced. Macrophages retrieved from IBR-immune and -susceptible donors were affected to a similar extent. The ability of macrophages to participate in antibody-dependent cell cytotoxicity was reduced dramatically from 2 h after IBR virus infection, suggesting that IBR virus-infected alveolar macrophages undergo alterations in immunological activity long before morphological changes in the cells become apparent.

Full text

PDF

Images in this article

1045Image
on p.1045

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Daughaday C. C., Douglas S. D. Membrane receptors on rabbit and human pulmonary alveolar macrophages. J Reticuloendothel Soc. 1976 Jan;19(1):37–45. [PubMed] [Google Scholar]
  3. Forman A. J., Babiuk L. A., Misra V., Baldwin F. Susceptibility of bovine macrophages to infectious bovine rhinotracheitis virus infection. Infect Immun. 1982 Mar;35(3):1048–1057. doi: 10.1128/iai.35.3.1048-1057.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grewal A. S., Babiuk L. A. Complement-dependent, polymorphonuclear neutrophil-mediated cytotoxicity of herpesvirus-infected cells: possible mechanism(s) of cytotoxicity. Immunology. 1980 Jun;40(2):151–161. [PMC free article] [PubMed] [Google Scholar]
  5. Grewal A. S., Rouse B. T., Babiuk L. A. Mechanisms of recovery from viral infections: destruction of infected cells by neutrophils and complement. J Immunol. 1980 Jan;124(1):312–319. [PubMed] [Google Scholar]
  6. Hearst J. E., Warr G. A., Jakab G. J. Characterization of murine lung and peritoneal macrophages. J Reticuloendothel Soc. 1980 May;27(5):443–454. [PubMed] [Google Scholar]
  7. Hocking W. G., Golde D. W. The pulmonary-alveolar macrophage (first of two parts). N Engl J Med. 1979 Sep 13;301(11):580–587. doi: 10.1056/NEJM197909133011104. [DOI] [PubMed] [Google Scholar]
  8. Howard C. J., Taylor G., Brownlie J. Surface receptors for immunoglobulin on bovine polymorphonuclear neutrophils and macrophages. Res Vet Sci. 1980 Jul;29(1):128–130. [PubMed] [Google Scholar]
  9. Jakab G. J., Green G. M. The effect of Sendai virus infection on bactericidal and transport mechanisms of the murine lung. J Clin Invest. 1972 Aug;51(8):1989–1998. doi: 10.1172/JCI107005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jericho K. W., Darcel C. Q. Response of the respiratory tract of calves kept at controlled climatic conditions to bovine Herpesvirus 1 in aerosol. Can J Comp Med. 1978 Apr;42(2):156–167. [PMC free article] [PubMed] [Google Scholar]
  11. Jericho K. W., Langford E. V. Pneumonia in calves produced with aerosols of bovine herpesvirus 1 and Pasteurella haemolytica. Can J Comp Med. 1978 Jul;42(3):269–277. [PMC free article] [PubMed] [Google Scholar]
  12. Loosli C. G. Synergism between respiratory viruses and bacteria. Yale J Biol Med. 1968 Apr-Jun;40(5-6):522–540. [PMC free article] [PubMed] [Google Scholar]
  13. Martin S. W., Meek A. H., Davis D. G., Thomson R. G., Johnson J. A., Lopez A., Stephens L., Curtis R. A., Prescott J. F., Rosendal S. Factors associated with mortality in feedlot cattle: the Bruce County Beef Cattle Project. Can J Comp Med. 1980 Jan;44(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  14. McConnell I., Klein G., Lint T. F., Lachmann P. J. Activation of the alternative complement pathway by human B cell lymphoma lines is associated with Epstein-Barr virus transformation of the cells. Eur J Immunol. 1978 Jul;8(7):453–458. doi: 10.1002/eji.1830080702. [DOI] [PubMed] [Google Scholar]
  15. Morrison R. P., Cutler J. E. In vitro studies of the interaction of murine phagocytic cells with Candida albicans. J Reticuloendothel Soc. 1981 Jan;29(1):23–34. [PubMed] [Google Scholar]
  16. Murphey S. A., Root R. K., Schreiber A. D. The role of antibody and complement in phagocytosis by rabbit alveolar macrophages. J Infect Dis. 1979 Dec;140(6):896–903. doi: 10.1093/infdis/140.6.896. [DOI] [PubMed] [Google Scholar]
  17. Nugent K. M., Pesanti E. L. Effect of influenza infection on the phagocytic and bactericidal activities of pulmonary macrophages. Infect Immun. 1979 Nov;26(2):651–657. doi: 10.1128/iai.26.2.651-657.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reynolds H. Y., Atkinson J. P., Newball H. H., Frank M. M. Receptors for immunoglobulin and complement on human alveolar macrophages. J Immunol. 1975 Jun;114(6):1813–1819. [PubMed] [Google Scholar]
  19. Rodgers B., Mims C. A. Interaction of influenza virus with mouse macrophages. Infect Immun. 1981 Feb;31(2):751–757. doi: 10.1128/iai.31.2.751-757.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ross G. D., Polley M. J. Assay for the two different types of lymphocyte complement receptors. Scand J Immunol. 1976 Jun;Suppl 5:99–111. doi: 10.1111/j.1365-3083.1976.tb03861.x. [DOI] [PubMed] [Google Scholar]
  21. Roth J. A., Kaeberle M. L., Griffith R. W. Effects of bovine viral diarrhea virus infection on bovine polymorphonuclear leukocyte function. Am J Vet Res. 1981 Feb;42(2):244–250. [PubMed] [Google Scholar]
  22. Rouse B. T., Grewal A. S., Babiuk L. A., Fujimiya Y. Enhancement of antibody-dependent cell-mediated cytotoxicity of herpesvirus-infected cells by complement. Infect Immun. 1977 Dec;18(3):660–665. doi: 10.1128/iai.18.3.660-665.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rouse B. T., Wardley R. C., Babiuk L. A. Antibody-dependent cell-mediated cytotoxicity in cows: comparison of effector cell activity against heterologous erthrocyte and herpesvirus-infected bovine target cells. Infect Immun. 1976 May;13(5):1433–1441. doi: 10.1128/iai.13.5.1433-1441.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SELLERS T. F., Jr, SCHULMAN J., BOUVIER C., McCUNE R., KILBOURNE E. D. The influence of influenza virus infection on exogenous staphylococcal and endogenous murine bacterial infection of the bronchopulmonary tissues of mice. J Exp Med. 1961 Aug 1;114:237–256. doi: 10.1084/jem.114.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shanley J. D., Pesanti E. L. Replication of murine cytomegalovirus in lung macrophages: effect of phagocytosis of bacteria. Infect Immun. 1980 Sep;29(3):1152–1159. doi: 10.1128/iai.29.3.1152-1159.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Silverberg B. A., Jakab G. J., Thomson R. G., Warr G. A., Boo K. S. Ultrastructural alterations in phagocytic functions of alveolar macrophages after parainfluenza virus infection. J Reticuloendothel Soc. 1979 Apr;25(4):405–416. [PubMed] [Google Scholar]
  27. Thomson R. G., Benson M. L., Savan M. Pneumonic pasteurellosis of cattle: microbiology and immunology. Can J Comp Med. 1969 Jul;33(3):194–206. [PMC free article] [PubMed] [Google Scholar]
  28. Tsai K. S. Replication of parainfluenza type 3 virus in alveolar macrophages: evidence of in vivo infection and of in vitro temperature sensitivity in virus maturation. Infect Immun. 1977 Dec;18(3):780–791. doi: 10.1128/iai.18.3.780-791.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wardley R. C., Rouse B. T., Babiuk L. A. Antibody dependent cytotoxicity mediated by neutrophils: a possible mechanism of antiviral defense. J Reticuloendothel Soc. 1976 May;19(5):323–332. [PubMed] [Google Scholar]
  30. Wardley R. C., Rouse B. T., Babiuk L. A. The mammary gland of the ox: a convenient source for the repeated collection of neutrophils and macrophages. J Reticuloendothel Soc. 1976 Jan;19(1):29–36. [PubMed] [Google Scholar]
  31. Warr G. A., Jakab G. J. Alterations in lung macrophage antimicrobial activity associated with viral pneumonia. Infect Immun. 1979 Nov;26(2):492–497. doi: 10.1128/iai.26.2.492-497.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Warr G. A., Jakab G. J., Hearst J. E. Alterations in lung macrophage immune receptor(s) activity associated with viral pneumonia. J Reticuloendothel Soc. 1979 Oct;26(4):357–366. [PubMed] [Google Scholar]
  33. Warshauer D., Goldstein E., Akers T., Lippert W., Kim M. Effect of influenza viral infection on the ingestion and killing of bacteria by alveolar macrophages. Am Rev Respir Dis. 1977 Feb;115(2):269–277. doi: 10.1164/arrd.1977.115.2.269. [DOI] [PubMed] [Google Scholar]
  34. Westmoreland D., St Jeor S., Rapp F. The development by cytomegalovirus-infected cells of binding affinity for normal human immunoglobulin. J Immunol. 1976 Jun;116(6):1566–1570. [PubMed] [Google Scholar]
  35. Wilkie B. N., Markham R. J. Sequential titration of bovine lung and serum antibodies after parenteral or pulmonary inoculation with Pasteurella haemolytica. Am J Vet Res. 1979 Dec;40(12):1690–1693. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES