Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Mar;35(3):1058–1062. doi: 10.1128/iai.35.3.1058-1062.1982

Characterization of an antigenically distinct porcine rotavirus.

J C Bridger, I N Clarke, M A McCrae
PMCID: PMC351154  PMID: 6175575

Abstract

A porcine virus with rotavirus morphology, which was antigenically unrelated to previously described rotaviruses, is described. Particles with an outer capsid layer measured 75 nm and those lacking the outer layer were 63 nm in diameter. Particles which resembled cores were also identified. The virus was shown to be antigenically distinct from other rotaviruses as judged by immunofluorescence and immune electron microscopy, and it failed to protect piglets from challenge with porcine rotavirus. Analysis of the genome indicated that it was composed of 11 segments of double-stranded RNA with the same overall size range as other rotaviruses but with an unusual segment pattern.

Full text

PDF
1058

Images in this article

1059Image
on p.1059
1060Image
on p.1060
1061Image
on p.1061

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Bradburne A. F., Wreghitt T. G. The effect of sodium thiocyanate on virus structure. J Med Virol. 1979;4(4):269–277. doi: 10.1002/jmv.1890040405. [DOI] [PubMed] [Google Scholar]
  2. Bridger J. C., Brown J. F. Development of immunity to porcine rotavirus in piglets protected from disease by bovine colostrum. Infect Immun. 1981 Mar;31(3):906–910. doi: 10.1128/iai.31.3.906-910.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bridger J. C. Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Vet Rec. 1980 Dec 6;107(23):532–533. [PubMed] [Google Scholar]
  4. Bridger J. C. Location of type-specific antigens in calf rotaviruses. J Clin Microbiol. 1978 Dec;8(6):625–628. doi: 10.1128/jcm.8.6.625-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bridger J. C., Woode G. N. Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. Br Vet J. 1975 Sep-Oct;131(5):528–535. [PubMed] [Google Scholar]
  6. Clarke I. N., McCrae M. A. A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus. J Virol Methods. 1981 Mar;2(4):203–209. doi: 10.1016/0166-0934(81)90010-0. [DOI] [PubMed] [Google Scholar]
  7. Espejo R. T., Muñz O., Serafin F., Romero P. Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infect Immun. 1980 Feb;27(2):351–354. doi: 10.1128/iai.27.2.351-354.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. McNulty M. S. Rotaviruses. J Gen Virol. 1978 Jul;40(1):1–18. doi: 10.1099/0022-1317-40-1-1. [DOI] [PubMed] [Google Scholar]
  11. Rodger S. M., Holmes I. H. Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol. 1979 Jun;30(3):839–846. doi: 10.1128/jvi.30.3.839-846.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Saif L. J., Bohl E. H., Theil K. W., Cross R. F., House J. A. Rotavirus-like, calicivirus-like, and 23-nm virus-like particles associated with diarrhea in young pigs. J Clin Microbiol. 1980 Jul;12(1):105–111. doi: 10.1128/jcm.12.1.105-111.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tavernor W. D., Trexler P. C., Vaughan L. C., Jones D. G. The production of gnotobiotic piglets and calves by hysterotomy under general anaesthesia. Vet Rec. 1971 Jan 2;88(1):10–14. doi: 10.1136/vr.88.1.10. [DOI] [PubMed] [Google Scholar]
  14. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Woode G. N., Bridger J., Hall G. A., Jones J. M., Jackson G. The isolation of reovirus-like agents (rota-viruses) from acute gastroenteritis of piglets. J Med Microbiol. 1976 May;9(2):203–209. doi: 10.1099/00222615-9-2-203. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES