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Fibroblast spreading on 0.5 – 8.5 kPa polyacrylamide gels crosslinked with 50 μg/ml fibronectin.
BR5 fibroblasts were cultured 4 and 16 hr in FBS and PDGF-containing medium on 0.5 – 8.5 kPa polyacrylamide gels as indicated with fibronectin crosslinked at 50 μg/ml. At the end of incubations, samples were fixed and stained for actin. Scale bar, 100 μm.
