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Effect of blebbistatin on fibroblast spreading in FBS-containing medium.
BR5 fibroblasts were cultured 4 hr in FBS-containing medium with and without 20 μM blebbistatin (Bl) on 0.5 – 8.5 kPa polyacrylamide gels as indicated with fibronectin and collagen crosslinked at 50 μg/ml. At the end of incubations, samples were fixed and stained for actin. Scale bar, 100 μm.
