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. 1982 Apr;36(1):1–10. doi: 10.1128/iai.36.1.1-10.1982

Immunization of cattle with a variant-specific surface antigen of Trypanosoma brucei: influence of different adjuvants.

P W Wells, D L Emery, C A Hinson, W I Morrison, M Murray
PMCID: PMC351176  PMID: 6281183

Abstract

Nine different adjuvants were examined for their ability to potentiate the humoral and cell-mediated immune responses of cattle to a soluble glycoprotein antigen prepared from Trypanosoma brucei. Serological responses as measured by the Farr assay were best augmented by the oil-based adjuvants and saponin. Cell-mediated immunity, as assessed by specific lymphocyte transformation in vitro, was enhanced by all oil-based adjuvants at different intervals after immunization. Results from a challenge infection of immunized cattle with the homologous clone of T. brucei and from neutralization tests indicated that protection against infection was better correlated with specific antibodies than with cell-mediated responses. From these considerations, and the absence of tissue reactions at the site of inoculation, saponin was considered more practical than the oil-based or bacterial adjuvants for the elicitation in cattle of antibodies to purified soluble antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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