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. 1982 Apr;36(1):310–319. doi: 10.1128/iai.36.1.310-319.1982

Ultrastructural study of long-term canine distemper virus infection in tissue culture cells.

H K Narang
PMCID: PMC351220  PMID: 7076301

Abstract

The morphogenesis of canine distemper virus was studied in Vero cell cultures for 43 days post-inoculation. Active replication of the virus was observed by electron microscopy and assay from 12 h after inoculation on, and peak production was observed on days 5, 14, and 22. From day 28 on, constant but smaller amounts of infectious virus were detected. Two ultrastructural types of intracytoplasmic nucleoprotein filaments were observed; although they first appeared at different times, their subsequent chronological patterns of development were similar. The cells apparently became free of virus by a mechanism of vacuolation. Intranuclear filaments were seen about day 11 and appeared to increase in number thereafter, whereas the infectious titer declined. Possible mechanisms of persistence are discussed in the light of these findings.

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Selected References

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  1. Adams D. H., Bell T. M. The relationship between measles virus infection and subacute sclerosing panencephalitis (SSPE). Med Hypotheses. 1976 Mar-Apr;2(2):55–57. doi: 10.1016/0306-9877(76)90062-1. [DOI] [PubMed] [Google Scholar]
  2. Byington D. P., Johnson K. P. Experimental subacute sclerosing panencephalitis in the hamster: correlation of age with chronic inclusion-cell encephalitis. J Infect Dis. 1972 Jul;126(1):18–26. doi: 10.1093/infdis/126.1.18. [DOI] [PubMed] [Google Scholar]
  3. Byington D. P., Johnson K. P. Subacute sclerosing panencephalitis (SSPE) agent in hamsters. II. The neuropathology of acute and chronic infections. Exp Mol Pathol. 1973 Jun;18(3):345–356. doi: 10.1016/0014-4800(73)90030-0. [DOI] [PubMed] [Google Scholar]
  4. Chen T. T., Watanabe I., Zeman W., Mealey J., Jr Subacute sclerosing panencephalitis: propagation of measles virus from brain biopsy in tissue culture. Science. 1969 Mar 14;163(3872):1193–1194. doi: 10.1126/science.163.3872.1193. [DOI] [PubMed] [Google Scholar]
  5. Connolly J. H., Allen I. V., Hurwitz L. J., Millar J. H. Measles-virus antibody and antigen in subacute sclerosing panencephalitis. Lancet. 1967 Mar 11;1(7489):542–544. doi: 10.1016/s0140-6736(67)92117-4. [DOI] [PubMed] [Google Scholar]
  6. Horta-Barbosa L., Fuccillo D. A., Sever J. L., Zeman W. Subacute sclerosing panencephalitis: isolation of measles virus from a brain biopsy. Nature. 1969 Mar 8;221(5184):974–974. doi: 10.1038/221974a0. [DOI] [PubMed] [Google Scholar]
  7. ISAACS A., LINDENMANN J. Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):258–267. doi: 10.1098/rspb.1957.0048. [DOI] [PubMed] [Google Scholar]
  8. Imagawa D. T. Relationships among measles, canine distemper and rinderpest viruses. Prog Med Virol. 1968;10:160–193. [PubMed] [Google Scholar]
  9. Katz M., Oyanagi S., Koprowski H. Subacute sclerosing panencephalitis: structures resembling myxovirus nucleocapsids in cells cultured from brain. Nature. 1969 May 31;222(5196):888–890. doi: 10.1038/222888a0. [DOI] [PubMed] [Google Scholar]
  10. Koestner A., Long J. F. Ultrastructure of canine distemper virus in explant tissue cultures of canine cerebellum. Lab Invest. 1970 Aug;23(2):196–201. [PubMed] [Google Scholar]
  11. Kolár O., Zeman W. Immunoelectrophoretic changes of serum IgG during subacute inflammatory and demyelinating diseases of the central nervous system. Z Immunitatsforsch Allerg Klin Immunol. 1967 Dec;134(3):267–275. [PubMed] [Google Scholar]
  12. Narang H. K. Comparative morphology of measles virus and paramyxovirus-like tubules in multiple sclerosis using ruthenium red stain. Neuropathol Appl Neurobiol. 1981 Sep-Oct;7(5):411–420. doi: 10.1111/j.1365-2990.1981.tb00242.x. [DOI] [PubMed] [Google Scholar]
  13. Narang H. K. Virus-like particles in natural scrapie of the sheep. Res Vet Sci. 1973 Jan;14(1):108–110. [PubMed] [Google Scholar]
  14. Oyanagi S., ter Meulen V., Katz M., Koprowski H. Comparison of subacute sclerosing panencephalitis and measles viruses: an electron microscope study. J Virol. 1971 Jan;7(1):176–187. doi: 10.1128/jvi.7.1.176-187.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Oyanagi S., ter Meulen V., Müller D., Katz M., Koprowski H. Electron microscopic observations in subacute sclerosing panencephalitis brain cell cultures: their correlation with cytochemical and immunocytological findings. J Virol. 1970 Sep;6(3):370–379. doi: 10.1128/jvi.6.3.370-379.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Payne F. E., Baublis J. V., Itabashi H. H. Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. N Engl J Med. 1969 Sep 11;281(11):585–589. doi: 10.1056/NEJM196909112811103. [DOI] [PubMed] [Google Scholar]
  17. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. Ultrastructural study of long-term measles infection in cultures of hamster dorsal-root ganglion. J Virol. 1971 Sep;8(3):318–329. doi: 10.1128/jvi.8.3.318-329.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wiśniewski H., Raine C. S., Kay W. J. Observations on viral demyelinating encephalomyelitis. Canine distemper. Lab Invest. 1972 May;26(5):589–599. [PubMed] [Google Scholar]
  19. Wong K. T., Baron S., Ward T. G. Rubella virus: role of interferon during infection of African green monkey kidney tissue cultures. J Immunol. 1967 Dec;99(6):1140–1149. [PubMed] [Google Scholar]
  20. Zhdanov V. M. Integration of viral genomes. Nature. 1975 Aug 7;256(5517):471–473. doi: 10.1038/256471a0. [DOI] [PubMed] [Google Scholar]
  21. ter Meulen V., Müller D., Katz M., Käckell M. Y., Joppich G. Immunohistological, microscical and neurochemical studies on encephalitides. IV. Subacute sclerosing (progressive) panencephalitis. Histochemical and immunohistological findings in tissue cultures derived from SSPE brain biopsies. Acta Neuropathol. 1970;15(1):1–10. doi: 10.1007/BF00690684. [DOI] [PubMed] [Google Scholar]

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