Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 May;36(2):462–468. doi: 10.1128/iai.36.2.462-468.1982

F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects.

P Klemm, I Orskov, F Orskov
PMCID: PMC351250  PMID: 6123483

Abstract

Fimbriae from three Escherichia coli strains, C1212, C1214, and C1023, isolated from urinary tract infections, have been purified and characterized by determination of the N-terminal sequences, amino acid composition, and molecular weights of their respective subunits. Furthermore, their immunological interrelationships have been investigated. The three strains all harbored more than one fimbrial species each. Immunologically different type 1-like fimbriae, termed 1A, 1B, and 1C, with highly homologous N-terminal sequences were isolated, of which strain C1212 possessed 1A and 1C, strain C1214 possessed 1A and 1B, and strain C1023 possessed 1A and 1C. Type 1A is known to cause a mannose-sensitive hemagglutination similar to that described for type 1 fimbriae, whereas the functions of types 1B and 1C are not yet known. Strain C1212, in addition, harbored the F7 fimbrial antigen which causes mannose-resistant hemagglutination and adherence to urinary epithelial cells. The N-terminal structure of this antigen seems to indicate a possible evolutionary kinship to the type 1-like fimbriae, although they are immunologically unrelated. Our results indicate that fimbriation of pathogenic wild-type strains can be of an intricate variety.

Full text

PDF
462

Images in this article

464Image
on p.464
467Image
on p.467

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsen N. H., Bock E., Kroll J. Comparison of antigens: the reaction of 'identity'. Scand J Immunol Suppl. 1973;1:91–94. doi: 10.1111/j.1365-3083.1973.tb03785.x. [DOI] [PubMed] [Google Scholar]
  2. Edén C. S., Hanson L. A., Jodal U., Lindberg U., Akerlund A. S. Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976 Sep 4;1(7984):490–492. [PubMed] [Google Scholar]
  3. Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eng P. R., Parkes C. O. SDS electrophoresis of fluorescamine-labeled proteins. Anal Biochem. 1974 May;59(1):323–325. doi: 10.1016/0003-2697(74)90041-4. [DOI] [PubMed] [Google Scholar]
  5. Evans D. G., Evans D. J., Jr, DuPont H. L. Virulence factors of enterotoxigenic Escherichia coli. J Infect Dis. 1977 Aug;136 (Suppl):S118–S123. doi: 10.1093/infdis/136.supplement.s118. [DOI] [PubMed] [Google Scholar]
  6. Hermodson M. A., Chen K. C., Buchanan T. M. Neisseria pili proteins: amino-terminal amino acid sequences and identification of an unusual amino acid. Biochemistry. 1978 Feb 7;17(3):442–445. doi: 10.1021/bi00596a010. [DOI] [PubMed] [Google Scholar]
  7. Klemm P. Fimbrial colonization factor CFA/1 protein from human enteropathogenic Escherichia coli strains. Purification, characterization and N-terminal sequence. FEBS Lett. 1979 Dec 1;108(1):107–110. doi: 10.1016/0014-5793(79)81188-6. [DOI] [PubMed] [Google Scholar]
  8. Klemm P., Poulsen F., Harboe M. K., Foltmann B. N-terminal amino acid sequences of pepsinogens from dogfish and seal and of bovine pepsinogen B. Acta Chem Scand B. 1976;30(10):979–984. doi: 10.3891/acta.chem.scand.30b-0979. [DOI] [PubMed] [Google Scholar]
  9. Klemm P. The complete amino-acid sequence of the K88 antigen, a fimbrial protein from Escherichia coli. Eur J Biochem. 1981 Jul;117(3):617–627. doi: 10.1111/j.1432-1033.1981.tb06382.x. [DOI] [PubMed] [Google Scholar]
  10. Kroll J. Crossed-line immunoelectrophoresis (73, 76). Scand J Immunol Suppl. 1973;1:79–81. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Orskov I., Ferencz A., Orskov F. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriated Escherichia coli. Lancet. 1980 Apr 19;1(8173):887–887. doi: 10.1016/s0140-6736(80)91396-3. [DOI] [PubMed] [Google Scholar]
  13. Orskov I., Orskov F., Birch-Andersen A. Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infect Immun. 1980 Feb;27(2):657–666. doi: 10.1128/iai.27.2.657-666.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weeke B. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures. Scand J Immunol Suppl. 1973;1:15–35. doi: 10.1111/j.1365-3083.1973.tb03776.x. [DOI] [PubMed] [Google Scholar]
  15. Weeke B. Crossed immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:47–56. doi: 10.1111/j.1365-3083.1973.tb03778.x. [DOI] [PubMed] [Google Scholar]
  16. de Graaf F. K., Klemm P., Gaastra W. Purification, characterization, and partial covalent structure of Escherichia coli adhesive antigen K99. Infect Immun. 1981 Sep;33(3):877–883. doi: 10.1128/iai.33.3.877-883.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES