Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 May;36(2):531–534. doi: 10.1128/iai.36.2.531-534.1982

Energy metabolism of the contagious equine metritis bacterium.

D G Lindmark, E L Jarroll, P J Timoney, S J Shin
PMCID: PMC351260  PMID: 7085071

Abstract

The energy metabolism of the English E-CMO strain of contagious equine metritis bacterium was studied in whole cells and cell extracts. This bacterium appears to have an active Krebs cycle and probably obtains energy by oxidative phosphorylation since glycolysis and the hexose monophosphate pathways appear to be absent. These conclusions are based on the findings that [U-14C]glucose incorporation by this bacterium is below the level of detection, and that respiration is stimulated by Krebs cycle intermediates (i.e., malate, citrate, and succinate), but not by glucose, fructose, maltose, or sucrose. Furthermore, support comes from the fact that enzymes generally associated with the Krebs cycle and electron transport (i.e., malate dehydrogenase, succinate dehydrogenase, isocitrate dehydrogenase, fumarate hydratase, malate dehydrogenase [decarboxylating], cytochrome oxidase, superoxide dismutase, NADH dehydrogenase, and catalase) were detected. Those enzymes normally associated with glycolysis and the hexose monophosphate pathways (i.e., hexokinase, glucose 6-phosphate dehydrogenase, fructose biphosphate aldolase, glycerol 3-phosphate dehydrogenase, phosphoenolpyruvate carboxykinase, pyruvate kinase, phosphate acetyl transferase, acetate kinase, alcohol dehydrogenase, and lactate dehydrogenase) were below the level of detection.

Full text

PDF
531

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crowhurst R. C. Genital infection in mares. Vet Rec. 1977 May 28;100(22):476–476. doi: 10.1136/vr.100.22.476-a. [DOI] [PubMed] [Google Scholar]
  2. Dabernat H. J., Delmas C. F., Tainturier D. J., Lareng M. B. In vitro susceptibility of Haemophilus equigenitalis, the causative organism of contagious equine metritis 1977, to antimicrobial agents. Antimicrob Agents Chemother. 1980 Dec;18(6):841–843. doi: 10.1128/aac.18.6.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hughes K. L., Bryden J. D., Macdonald F. Equine contagious metritis. Aust Vet J. 1978 Feb;54(2):101–101. doi: 10.1111/j.1751-0813.1978.tb00365.x. [DOI] [PubMed] [Google Scholar]
  4. Lindmark D. G. Energy metabolism of the anaerobic protozoon Giardia lamblia. Mol Biochem Parasitol. 1980 Mar;1(1):1–12. doi: 10.1016/0166-6851(80)90037-7. [DOI] [PubMed] [Google Scholar]
  5. Lindmark D. G., Müller M. Superoxide dismutase in the anaerobic flagellates, Tritrichomonas foetus and Monocercomonas sp. J Biol Chem. 1974 Jul 25;249(14):4634–4637. [PubMed] [Google Scholar]
  6. Mackintosh M. E. Bacteriological techniques in the diagnosis of equine genital infections. Vet Rec. 1981 Jan 17;108(3):52–55. doi: 10.1136/vr.108.3.52. [DOI] [PubMed] [Google Scholar]
  7. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mumme J., Ahlswede L. Nachweis von Haemophilus equigenitalis im Zervixtupfer einer Warmblutstute. Dtsch Tierarztl Wochenschr. 1979 Jul 5;86(7):257–259. [PubMed] [Google Scholar]
  9. Müller M. Biochemical cytology of trichomonad flagellates. I. Subcellular localization of hydrolases, dehydrogenases, and catalase in Tritrichomonas foetus. J Cell Biol. 1973 May;57(2):453–474. doi: 10.1083/jcb.57.2.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Müller M., Hogg J. F., De Duve C. Distribution of tricarboxylic acid cycle enzymes and glyoxylate cycle enzymes between mitochondria and peroxisomes in Tetrahymena pyriformis. J Biol Chem. 1968 Oct 25;243(20):5385–5395. [PubMed] [Google Scholar]
  11. O'Driscoll J. G., Troy P. T., Geoghegan F. J. An epidemic of venereal infection in thoroughbreds. Vet Rec. 1977 Oct 29;101(18):359–360. doi: 10.1136/vr.101.18.359. [DOI] [PubMed] [Google Scholar]
  12. Platt H., Atherton J. G., Simpson D. J., Taylor C. E., Rosenthal R. O., Brown D. F., Wreghitt T. G. Genital infection in mares. Vet Rec. 1977 Jul 2;101(1):20–20. doi: 10.1136/vr.101.1.20. [DOI] [PubMed] [Google Scholar]
  13. Powell D. G. Contagious equine metritis. Equine Vet J. 1978 Jan;10(1):1–4. doi: 10.1111/j.2042-3306.1978.tb02200.x. [DOI] [PubMed] [Google Scholar]
  14. Sugimoto C., Isayama Y., Kashiwazaki M., Fujikura T., Mitani K. Detection of Haemophilus equigenitalis, the causal agent of contagious equine metritis, in Japan. Natl Inst Anim Health Q (Tokyo) 1980 Fall;20(3):118–119. [PubMed] [Google Scholar]
  15. Swerczek T. W. The first occurrence of contagious equine metritis in the United States. J Am Vet Med Assoc. 1978 Aug 15;173(4):405–407. [PubMed] [Google Scholar]
  16. Tainturier D. J., Delmas C. F., Dabernat H. J. Bacteriological and serological studies of haemophilus equigenitalis, agent of contagious equine metritis. J Clin Microbiol. 1981 Oct;14(4):355–360. doi: 10.1128/jcm.14.4.355-360.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor C. E., Rosenthal R. O., Brown D. F., Lapage S. P., Hill L. R., Legros R. M. The causative organism of contagious equine metritis 1977: proposal for a new species to be known as Haemophilus equigenitalis. Equine Vet J. 1978 Jul;10(3):136–144. doi: 10.1111/j.2042-3306.1978.tb02242.x. [DOI] [PubMed] [Google Scholar]
  18. Timoney P. J., Ward J., Kelly P. A contagious genital infection of mares. Vet Rec. 1977 Jul 30;101(5):103–103. doi: 10.1136/vr.101.5.103-a. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES