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. 1982 May;36(2):591–597. doi: 10.1128/iai.36.2.591-597.1982

Chemotaxis of purified human monocytes in vitro: lack of accessory cell requirement.

W Falk, E J Leonard
PMCID: PMC351269  PMID: 7085073

Abstract

This study was undertaken to determine whether cell cooperation, either among monocytes or between monocytes and lymphocytes, is a prerequisite for monocyte chemotactic responsiveness. We compared Ficoll-Hypaque-separated mononuclear cells and a preparation of 99% pure monocytes obtained by chemotaxis in a newly designed separation chamber. Monocytes of both preparations migrated to chemoattractants without a lag phase, and no further increase in migrated cells was observed after 70 min. The cell dose-response was linear for both preparations over a wide range of cell concentrations in the cell input well of the chemotaxis chamber, suggesting that no monocyte-monocyte interaction was required. Since only 20 to 60% of the monocytes purified by chemotaxis migrated a second time, the possibility of a requirement for an accessory cell was tested. The addition to purified monocytes of several different mononuclear cell preparations comprising lymphocytes or nonmigrating monocytes had no effect on monocyte migration. These experiments show that normal human blood monocytes in vitro do not require stimuli from other cells to respond to chemoattractants. Their behavior is profoundly different from that of mouse peritoneal macrophages, which exhibit a time lag in vitro before migration toward an attractant and become more responsive with either increasing cell concentration or addition of purified lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aksamit R. R., Falk W., Leonard E. J. Chemotaxis by mouse macrophage cell lines. J Immunol. 1981 Jun;126(6):2194–2199. [PubMed] [Google Scholar]
  2. Altman L. C., Furukawa C. T., Klebanoff S. J. Depressed mononuclear leukocyte chemotaxis in thermally injured patients. J Immunol. 1977 Jul;119(1):199–205. [PubMed] [Google Scholar]
  3. Boetcher D. A., Leonard E. J. Abnormal monocyte chemotactic response in cancer patients. J Natl Cancer Inst. 1974 Apr;52(4):1091–1099. doi: 10.1093/jnci/52.4.1091. [DOI] [PubMed] [Google Scholar]
  4. Boetcher D. A., Meltzer M. S. Mouse mononuclear cell chemotaxis: description of system. J Natl Cancer Inst. 1975 Mar;54(3):795–799. [PubMed] [Google Scholar]
  5. Boumsell L., Meltzer M. S. Mouse mononuclear cell chemotaxis. I. Differential response of monocytes and macrophages. J Immunol. 1975 Dec;115(6):1746–1748. [PubMed] [Google Scholar]
  6. Falk W., Goodwin R. H., Jr, Leonard E. J. A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 1980;33(3):239–247. doi: 10.1016/0022-1759(80)90211-2. [DOI] [PubMed] [Google Scholar]
  7. Falk W., Leonard E. J. Specificity and reversibility of chemotactic deactivation of human monocytes. Infect Immun. 1981 May;32(2):464–468. doi: 10.1128/iai.32.2.464-468.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fernandez H. N., Hugli T. E. Partial characterization of human C5a anaphylatoxin. I. Chemical description of the carbohydrate and polypeptide prtions of human C5a. J Immunol. 1976 Nov;117(5 Pt 1):1688–1694. [PubMed] [Google Scholar]
  9. Harvath L., Falk W., Leonard E. J. Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly. J Immunol Methods. 1980;37(1):39–45. doi: 10.1016/0022-1759(80)90179-9. [DOI] [PubMed] [Google Scholar]
  10. Lake W. W., Bice D., Schwartz H. J., Salvaggio J. Suppression of in vitro antigen-induced lymphocyte transformation by carrageenan, a macrophage-toxic agent. J Immunol. 1971 Dec;107(6):1745–1751. [PubMed] [Google Scholar]
  11. Leonard E. J., Skeel A. Effects of cell concentration on chemotactic responsiveness of mouse resident peritoneal macrophages. J Reticuloendothel Soc. 1981 Oct;30(4):271–282. [PubMed] [Google Scholar]
  12. Norris D. A., Perez R. E., Golitz L. E., Seitz L. E., Weston W. L. Defective monocyte chemotaxis in mycosis fungoides: lack of essential helper lymphocytes. Cancer. 1979 Jul;44(1):124–130. doi: 10.1002/1097-0142(197907)44:1<124::aid-cncr2820440122>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  13. Pasternack G. R., Snyderman R., Pike M. C., Johnson R. J., Shin H. S. Resistance of neoplasms to immunological destruction: role of a macrophage chemotaxis inhibitor. J Exp Med. 1978 Jul 1;148(1):93–102. doi: 10.1084/jem.148.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pertoft H., Johnsson A., Wärmegård B., Seljelid R. Separation of human monocytes on density gradients of Percoll. J Immunol Methods. 1980;33(3):221–229. doi: 10.1016/0022-1759(80)90209-4. [DOI] [PubMed] [Google Scholar]
  15. Pike M. C., Snyderman R. Depression of macrophage function by a factor produced by neoplasms: a merchanism for abrogation of immune surveillance. J Immunol. 1976 Oct;117(4):1243–1249. [PubMed] [Google Scholar]
  16. Snyderman R., Pike M. C. An inhibitor of macrophage chemotaxis produced by neoplasms. Science. 1976 Apr 23;192(4237):370–372. doi: 10.1126/science.946556. [DOI] [PubMed] [Google Scholar]
  17. Territo M. C., Cline M. J. Monocyte function in man. J Immunol. 1977 Jan;118(1):187–192. [PubMed] [Google Scholar]

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