Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1982 May;36(2):834–836. doi: 10.1128/iai.36.2.834-836.1982

Enhancement of invasiveness of Yersinia enterocolitica and Escherichia coli in HEp-2 cells by centrifugation.

T Vesikari, J Bromirska, M Mäki
PMCID: PMC351304  PMID: 7044978

Abstract

Centrifugation enhanced the infectivity of invasive Escherichia coli and Yersinia enterocolitica for HEp-2 cells. Noninvasive bacteria were not endocytosed after centrifugation. The centrifugation procedure may increase the sensitivity of testing for bacterial invasiveness in cell culture without causing false-positive results.

Full text

PDF
834

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Darougar S., Cubitt S., Jones B. R. Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of Chlamydia. Br J Vener Dis. 1974 Aug;50(4):308–312. doi: 10.1136/sti.50.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Devenish J. A., Schiemann D. A. HeLa cell infection by Yersinia enterocolitica: evidence for lack of intracellular multiplication and development of a new procedure for quantitative expression of infectivity. Infect Immun. 1981 Apr;32(1):48–55. doi: 10.1128/iai.32.1.48-55.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DuPont H. L., Formal S. B., Hornick R. B., Snyder M. J., Libonati J. P., Sheahan D. G., LaBrec E. H., Kalas J. P. Pathogenesis of Escherichia coli diarrhea. N Engl J Med. 1971 Jul 1;285(1):1–9. doi: 10.1056/NEJM197107012850101. [DOI] [PubMed] [Google Scholar]
  4. Giannella R. A., Washington O., Gemski P., Formal S. B. Invasion of HeLa cells by Salmonella typhimurium: a model for study of invasiveness of Salmonella. J Infect Dis. 1973 Jul;128(1):69–75. doi: 10.1093/infdis/128.1.69. [DOI] [PubMed] [Google Scholar]
  5. Hale T. L., Formal S. B. Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. Infect Immun. 1981 Apr;32(1):137–144. doi: 10.1128/iai.32.1.137-144.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harris J. C., Dupont H. L., Hornick R. B. Fecal leukocytes in diarrheal illness. Ann Intern Med. 1972 May;76(5):697–703. doi: 10.7326/0003-4819-76-5-697. [DOI] [PubMed] [Google Scholar]
  7. Kapperud G. Studies on the pathogenicity of Yersinia enterocolitica and Y. enterocolitica-like bacteria. 2. Interaction with HeLa cells among environmental and human isolates from Scandinavia. Acta Pathol Microbiol Scand B. 1980 Oct;88(5):293–297. [PubMed] [Google Scholar]
  8. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
  10. Mehlman I. J., Eide E. L., Sanders A. C., Fishbein M., Aulisio C. C. Methodology for recognition of invasive potential of Escherichia coli. J Assoc Off Anal Chem. 1977 May;60(3):546–562. [PubMed] [Google Scholar]
  11. Mäki M., Grönroos P., Vesikari T. In vitro invasiveness of Yersinia enterocolitica isolated from children with diarrhea. J Infect Dis. 1978 Nov;138(5):677–680. doi: 10.1093/infdis/138.5.677. [DOI] [PubMed] [Google Scholar]
  12. Mäki M., Vesikari T., Grönroos P. Enterotoxigenic and invasive Escherichia coli as causes of childhood diarrhoea in Finland. Acta Paediatr Scand. 1980 Mar;69(2):219–224. doi: 10.1111/j.1651-2227.1980.tb07064.x. [DOI] [PubMed] [Google Scholar]
  13. Mäki M., Vesikari T., Rantala I., Grönroos P. Yersiniosis in children. Arch Dis Child. 1980 Nov;55(11):861–865. doi: 10.1136/adc.55.11.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pedersen K. B., Winblad S., Bitsch V. Studies on the interaction between different O-serotypes of Yersinia enterocolitica and HeLa cells. Acta Pathol Microbiol Scand B. 1979 Apr;87B(2):141–145. doi: 10.1111/j.1699-0463.1979.tb02417.x. [DOI] [PubMed] [Google Scholar]
  15. Portnoy D. A., Moseley S. L., Falkow S. Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981 Feb;31(2):775–782. doi: 10.1128/iai.31.2.775-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rudoy R. C. Enteroinvasive and enterotoxigenic Escherichia coli. Occurrence in acute diarrhea of infants and children. Am J Dis Child. 1975 Jun;129(6):668–672. doi: 10.1001/archpedi.1975.02120430008004. [DOI] [PubMed] [Google Scholar]
  17. SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
  18. Une T. Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro. Microbiol Immunol. 1977;21(7):365–377. doi: 10.1111/j.1348-0421.1977.tb00301.x. [DOI] [PubMed] [Google Scholar]
  19. Vesikari T., Nurmi T., Mäki M., Skurnik M., Sundqvist C., Granfors K., Grönroos P. Plasmids in Yersinia enterocolitica serotypes O:3 and O:9: correlation with epithelial cell adherence in vitro. Infect Immun. 1981 Sep;33(3):870–876. doi: 10.1128/iai.33.3.870-876.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES