Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Feb;31(2):584–591. doi: 10.1128/iai.31.2.584-591.1981

Interaction between a trachoma strain of Chlamydia trachomatis and mouse fibroblasts (McCoy cells) in the absence of centrifugation.

C K Lee
PMCID: PMC351349  PMID: 7216462

Abstract

A system was devised for studying the interaction of a trachoma strain of Chlamydia trachomatis (G17) and mouse fibroblasts (McCoy cells) in the absence of centrifugation, which is usually employed to enhance the infection of cell cultures with non-lymphogranuloma venereum human strains of C. trachomatis. In this system, the conditions of infection more closely approached those encountered in natural infections, and the entry of G17 into host cells could be compared with the previously described entry of C. psittaci 6BC and a lymphogranuloma venereum strain (440L) of C. trachomatis. McCoy cells were infected by shaking at 37 degrees C with inocula suspended in 0.01 M phosphate buffer, pH 7.2, containing 0.2 M sucrose. The efficiency of infection (inclusion counts without centrifugation/inclusion counts with centrifugation) was 1.5% for monolayers and 7.5% for suspensions. When measured either by inclusion counts or by host cell-associated 14C-amino acid-labeled G17, association was proportional to G17 concentration and increased linearly for 60 min. Pretreatment of host cells with diethylaminoethyl-dextran (30 micrograms/ml, 30 min) raised the efficiency of infection to about 13% for both monolayers and suspensions. Host cells treated with cytochalasin B (2 x 10(-5) M, 90 min) or trypsin (50 micrograms/ml, 60 min) associated with G17 at undiminished rates. 14C-labeled G17 inactivated by heat (60 degrees C, 3 min) or ultraviolet light (1,800 ergs per mm2) associated with McCoy cells at the same rate as live G17. Comparison of these results with those previously reported for strains 6BC and 440L showed that strain G17 exhibited some, but not all, of the host cell association properties of the other two chlamydial strains.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander E. R., Wang S. P., Grayston J. T. Further classification of TRIC agents from ocular trachoma and other sources by the mouse toxicity prevention test. Am J Ophthalmol. 1967 May;63(5 Suppl):1469–1478. doi: 10.1016/0002-9394(67)94133-5. [DOI] [PubMed] [Google Scholar]
  2. BOVARNICK M. R., MILLER J. C. Oxidation and transamination of glutamate by typhus rickettsiae. J Biol Chem. 1950 Jun;184(2):661–676. [PubMed] [Google Scholar]
  3. Becker Y., Asher Y. Synthesis of trachoma agent proteins in emetine-treated cells. J Bacteriol. 1972 Mar;109(3):966–970. doi: 10.1128/jb.109.3.966-970.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Byrne G. I. Kinetics of phagocytosis of Chlamydia psittaci by mouse fibroblasts (L cells): separation of the attachment and ingestion stages. Infect Immun. 1978 Feb;19(2):607–612. doi: 10.1128/iai.19.2.607-612.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Byrne G. I., Moulder J. W. Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells. Infect Immun. 1978 Feb;19(2):598–606. doi: 10.1128/iai.19.2.598-606.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Byrne G. I. Requirements for ingestion of Chlamydia psittaci by mouse fibroblasts (L cells). Infect Immun. 1976 Sep;14(3):645–651. doi: 10.1128/iai.14.3.645-651.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Croy T. R., Kuo C. C., Wang S. P. Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms. J Clin Microbiol. 1975 May;1(5):434–439. doi: 10.1128/jcm.1.5.434-439.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Davies P., Allison A. C. Effects of cytochalasin B on endocytosis and exocytosis. Front Biol. 1978;46:143–160. [PubMed] [Google Scholar]
  9. Friis R. R. Interaction of L cells and Chlamydia psittaci: entry of the parasite and host responses to its development. J Bacteriol. 1972 May;110(2):706–721. doi: 10.1128/jb.110.2.706-721.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GORDON F. B., QUAN A. L. ISOLATION OF THE TRACHOMA AGENT IN CELL CULTURE. Proc Soc Exp Biol Med. 1965 Feb;118:354–359. doi: 10.3181/00379727-118-29841. [DOI] [PubMed] [Google Scholar]
  11. Grayston J. T., Wang S. New knowledge of chlamydiae and the diseases they cause. J Infect Dis. 1975 Jul;132(1):87–105. doi: 10.1093/infdis/132.1.87. [DOI] [PubMed] [Google Scholar]
  12. Gregory W. W., Byrne G. I., Gardner M., Moulder J. W. Cytochalasin B does not inhibit ingestion of Chlamydia psittaci by mouse fibroblasts (L cells) and mouse peritoneal macrophages. Infect Immun. 1979 Jul;25(1):463–466. doi: 10.1128/iai.25.1.463-466.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hale T. L., Bonventre P. F. Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infect Immun. 1979 Jun;24(3):879–886. doi: 10.1128/iai.24.3.879-886.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hale T. L., Morris R. E., Bonventre P. F. Shigella infection of henle intestinal epithelial cells: role of the host cell. Infect Immun. 1979 Jun;24(3):887–894. doi: 10.1128/iai.24.3.887-894.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hatch T. P. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect Immun. 1975 Jul;12(1):211–220. doi: 10.1128/iai.12.1.211-220.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnson F. W., Hobson D. Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents). J Hyg (Lond) 1976 Jun;76(3):441–451. doi: 10.1017/s0022172400055376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kuo C. C. Cultures of Chlamydia trachomatis in mouse peritoneal macrophages: factors affecting organism growth. Infect Immun. 1978 May;20(2):439–445. doi: 10.1128/iai.20.2.439-445.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kuo C. C., Grayston T. Interaction of Chlamydia trachomatis organisms and HeLa 229 cells. Infect Immun. 1976 Apr;13(4):1103–1109. doi: 10.1128/iai.13.4.1103-1109.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kuo C. C., Wang S. P., Grayston J. T. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction. Infect Immun. 1973 Jul;8(1):74–79. doi: 10.1128/iai.8.1.74-79.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rota T. R., Nichols R. L. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran. J Infect Dis. 1971 Oct;124(4):419–421. doi: 10.1093/infdis/124.4.419. [DOI] [PubMed] [Google Scholar]
  22. SOWA J., COLLIER L. H. Isolation of trachoma virus from patients in West Africa. J Hyg (Lond) 1960 Mar;58:99–108. doi: 10.1017/s0022172400038158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spears P., Storz J. Biotyping of Chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide. Infect Immun. 1979 Apr;24(1):224–232. doi: 10.1128/iai.24.1.224-232.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Walker T. S., Winkler H. H. Penetration of cultured mouse fibroblasts (L cells) by Rickettsia prowazeki. Infect Immun. 1978 Oct;22(1):200–208. doi: 10.1128/iai.22.1.200-208.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wentworth B. B., Alexander E. R. Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells. Appl Microbiol. 1974 May;27(5):912–916. doi: 10.1128/am.27.5.912-916.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES