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. 1981 Feb;31(2):608–614. doi: 10.1128/iai.31.2.608-614.1981

Common polysaccharide antigens from the cell envelope of Clostridium perfringens type A.

K I Dayalu, R Cherniak, C L Hatheway
PMCID: PMC351352  PMID: 6260671

Abstract

Soluble antigens were obtained by extracting five serotype strains of Clostridium perfringens type A with water at 100 degrees C. The type-specific polysaccharides were precipitated with ethanol, and the common antigens were recovered from the ethanol supernatants by concentration, dialysis, and lyophilization. Refluxing the water-extracted cell residues with 1% acetic acid followed by concentration, dialysis, and lyophilization gave additional common antigen fractions. A comprehensive, side-by-side comparison of the antigen fractions, the ethanol precipitate, the ethanol supernatant, and the acetic acid supernatant, revealed that common antigens were recovered in all three fractions, and that three distinct entities were responsible for the formation of the observed common immunoprecipitin lines; whereas many fractions possessed all three immunoprecipitin lines, others contained only one or two. The serological homology observed between the various antigen fractions was apparently a consequence of N-acetylglucosamine- and N-acetylmannosamine-containing polymers. The common antigens were presumably associated with the cell envelope and may be the type of markers sought previously by others for the serological identification of C. perfringens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  3. Baine H., Cherniak R. Capsular polysaccharides of Clostridium perfringens Hobbs 5. Biochemistry. 1971 Jul 20;10(15):2949–2952. [PubMed] [Google Scholar]
  4. Cherniak R., Frederick H. M. Capsular polysaccharide of Clostridium perfringens Hobbs 9. Infect Immun. 1977 Mar;15(3):765–771. doi: 10.1128/iai.15.3.765-771.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cherniak R., Henderson B. G. Immunochemistry of the capsular polysaccharides from Clostridium perfringens: selected Hobbs strains 1, 5, 9, and 10. Infect Immun. 1972 Jul;6(1):32–37. doi: 10.1128/iai.6.1.32-37.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cherniak R., Lombard G. L., Dowell V. R., Jr Immunochemical evidence for multiple serotypes of Bacteroides fragilis. J Clin Microbiol. 1979 Jun;9(6):699–704. doi: 10.1128/jcm.9.6.699-704.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOBBS B. C., SMITH M. E., OAKLEY C. L., WARRACK G. H., CRUICKSHANK J. C. Clostridium welchii food poisoning. J Hyg (Lond) 1953 Mar;51(1):75–101. doi: 10.1017/s0022172400015515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hughes J. A., Turnbull P. C., Stringer M. F. A serotyping system for Clostridium welchii (C. perfringens) type A, and studies on the type-specific antigens. J Med Microbiol. 1976 Nov;9(4):475–485. doi: 10.1099/00222615-9-4-475. [DOI] [PubMed] [Google Scholar]
  10. KLOTZ A. W. APPLICATION OF FA TECHNIQUES TO DETECTION OF CLOSTRIDIUM PERFRINGENS. Public Health Rep. 1965 Apr;80:305–311. [PMC free article] [PubMed] [Google Scholar]
  11. Lee L., Cherniak R. Capsular polysaccharide of Clostridium perfringens Hobbs 10. Infect Immun. 1974 Feb;9(2):318–322. doi: 10.1128/iai.9.2.318-322.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Paine C. M., Cherniak R. Composition of the capsular polysaccharides of Clostridium perfringens as a basis for their classification by chemotypes. Can J Microbiol. 1975 Feb;21(2):181–185. doi: 10.1139/m75-026. [DOI] [PubMed] [Google Scholar]
  13. Stringer M. F., Turnbull P. C., Gilbert R. J. Application of serological typing to the investigation of outbreaks of Clostridium perfringens food poisoning, 1970-1978. J Hyg (Lond) 1980 Jun;84(3):443–456. doi: 10.1017/s002217240002698x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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