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. 1981 Mar;31(3):1086–1093. doi: 10.1128/iai.31.3.1086-1093.1981

Binding of radiolabeled Staphylococcus aureus delta-toxin to human erythrocytes.

F S Nolte, F A Kapral
PMCID: PMC351428  PMID: 7228396

Abstract

The addition f [3H]isoleucine to a chemically defined medium resulted in the production of delta-toxin (DT) with a high specific radioactivity (0.47 microCi/mg). The purified tritium-labeled toxin ([3H]DT) was found to migrate in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band with a molecular weight of 1,600. Upon electrofocusing, [3H]DT yielded one major peak (pI = 5.90) and two minor peaks (pI = 5.10, 6.95) of radioactivity. The percentage of [3H]DT associated with pelleted fractions of intact erythrocytes or erythrocyte ghosts remained fairly constant over a 100-fold range of toxin concentrations. Erythrocyte ghosts, however, bound more [3H]DT than did intact erythrocytes when exposed to the same concentration of toxin. Erythrocytes maintained in isotonic sucrose were more susceptible to toxin than erythrocytes suspended in saline, but did not bind more [3H]DT. The binding of [3H]DT to erythrocyte ghosts was found to be temperature dependent from 0 to 20 degrees C but was constant from 20 to 50 degrees C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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