Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Mar;31(3):1145–1151. doi: 10.1128/iai.31.3.1145-1151.1981

Enhancement of delayed-type hypersensitivity to Trypanosoma cruzi in mice treated with Mycobacterium bovis BCG and cyclophosphamide.

I A Abrahamsohn, M H Blotta, M A Curotto
PMCID: PMC351437  PMID: 6785239

Abstract

C57BL/10J mice treated with Mycobacterium bovis BCG and cyclophosphamide were immunized with disrupted epimastigotes or with living blood trypomastigotes from Trypanosoma cruzi and assayed for delayed hypersensitivity by footpad testing with epimastigote antigens. Enhanced and lasting reactions were observed in mice pretreated with BCG or cyclophosphamide or both and immunized with epimastigotes. Whereas BCG pretreatment clearly reduced the mortality rates of mice immunized with living blood forms, no enhancement of the delayed hypersensitivity responses was observed in animals treated with BCG or cyclophosphamide or both before infection. The production of high levels of delayed hypersensitivity in the absence of infection and its adoptive transfer with cells could help to evaluate the participation of cell-mediated immunity in the protection against T. cruzi.

Full text

PDF
1145

Images in this article

1148Image
on p.1148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. Effect of cyclophosphamide treatment on the course of Mycobacterium lepraemurium infection and development of delayed-type hypersensitivity reactions in C57B1 and BALB/c mice. Clin Exp Immunol. 1978 Oct;34(1):52–58. [PMC free article] [PubMed] [Google Scholar]
  2. BRENER Z. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Rev Inst Med Trop Sao Paulo. 1962 Nov-Dec;4:389–396. [PubMed] [Google Scholar]
  3. Brener Z., Chiari E. The effects of some immunosuppressive agents in experimental chronic Chagas's disease. Trans R Soc Trop Med Hyg. 1971;65(5):629–636. doi: 10.1016/0035-9203(71)90047-2. [DOI] [PubMed] [Google Scholar]
  4. Burgess D. E., Hanson W. L. Adoptive transfer of protection against Trypanosoma cruzi with lymphocytes and macrophages. Infect Immun. 1979 Sep;25(3):838–843. doi: 10.1128/iai.25.3.838-843.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Camargo M. E. Fluorescent antibody test for the serodiagnosis of American trypanosomiasis. Technical modification employing preserved culture forms of Trypanosoma cruzi in a slide test. Rev Inst Med Trop Sao Paulo. 1966 Sep-Oct;8(5):227–235. [PubMed] [Google Scholar]
  6. Easmon C. S., Glynn A. A. Effect of cyclophosphamide on delayed hypersensitivity to Staphylococcus aureus in mice. Immunology. 1977 Nov;33(5):767–776. [PMC free article] [PubMed] [Google Scholar]
  7. González Cappa S. M., Pesce U. J., Cantarella A. I., Schmuñis G. A. Trypanosoma cruzi: protection of mice with epimastigote antigens from immunologically different parasite strains. Exp Parasitol. 1974 Apr;35(2):179–186. doi: 10.1016/0014-4894(74)90021-6. [DOI] [PubMed] [Google Scholar]
  8. Hoff R. Killing in vitro of Trypanosoma cruzi by macrophages from mice immunized with T. cruzi or BCG, and absence of cross-immunity on challege in vivo. J Exp Med. 1975 Aug 1;142(2):299–311. doi: 10.1084/jem.142.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kuhn R. E., Vaughn R. T., Herbst G. A. The effect of BCG on the course of experimental Chagas' disease in mice. Int J Parasitol. 1975 Oct;5(5):557–560. doi: 10.1016/0020-7519(75)90049-1. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lagrange P. H., Mackaness G. B., Miller T. E. Potentiation of T-cell-mediated immunity by selective suppression of antibody formation with cyclophosphamide. J Exp Med. 1974 Jun 1;139(6):1529–1539. doi: 10.1084/jem.139.6.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackaness G. B., Lagrange P. H., Ishibashi T. The modifying effect of BCG on the immunological induction of T cells. J Exp Med. 1974 Jun 1;139(6):1540–1552. doi: 10.1084/jem.139.6.1540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakamura R. M., Tokunaga T. Strain difference of delayed-type hypersensitivity to BCG and its genetic control in mice. Infect Immun. 1978 Dec;22(3):657–664. doi: 10.1128/iai.22.3.657-664.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Poulter L. W. Systemic immunological reactivity in the absence of delayed type hypersensitivity during murine leprosy. Cell Immunol. 1978 Sep 15;40(1):117–127. doi: 10.1016/0008-8749(78)90320-9. [DOI] [PubMed] [Google Scholar]
  15. Roberson E. L., Hanson W. L. Correspondence: Transfer of immunity to T. cruzi. Trans R Soc Trop Med Hyg. 1974;68(4):338–338. doi: 10.1016/0035-9203(74)90044-3. [DOI] [PubMed] [Google Scholar]
  16. Rowland E. C., Kuhn R. E. Suppression of anamnestic cellular responses during experimental American trypanosomiasis. J Parasitol. 1978 Aug;64(4):741–742. [PubMed] [Google Scholar]
  17. Schwartz A., Askenase P. W., Gershon R. K. Regulation of delayed-type hypersensitivity reactions by cyclophosphamide-sensitive T cells. J Immunol. 1978 Oct;121(4):1573–1577. [PubMed] [Google Scholar]
  18. Segura E. L., Vazquez C., Bronzina A., Campos J. M., Cerisola J. A., Cappa S. M. Antigens of the subcellular fractions of Trypanosoma cruzi. II. Flagellar and membrane fraction. J Protozool. 1977 Nov;24(4):540–543. doi: 10.1111/j.1550-7408.1977.tb01009.x. [DOI] [PubMed] [Google Scholar]
  19. Teixeira A. R., Santos-Buch C. A. The immunology of experimental Chagas' disease. II. Delayed hypersensitivity to Trypanosoma cruzi antigens. Immunology. 1975 Mar;28(3):401–410. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES