Abstract
Studies were conducted to determine the immunogenicity of purified Staphylococcus aureus delta-toxin. Rabbits and guinea pigs immunized with delta-toxin incorporated into a multiple antibody, whereas animals given toxin in saline or toxin in saline with Tween 80 did not produce antibody. The immunoglobulin G (IgG) fraction isolated by chromatography on protein A-Sepharose was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Although delta-toxin-specific IgG precipitated the toxin in agar gels, the antibody did not neutralize the toxin's hemolytic activity. Delta-toxin binding to human erythrocyte membranes was demonstrated by indirect immunofluorescent staining of toxin-treated erythrocytes.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birkbeck T. H., Whitelaw D. D. Immunogenicity and molecular characterisation of staphylococcal delta haemolysin. J Med Microbiol. 1980 May;13(2):213–221. doi: 10.1099/00222615-13-2-213. [DOI] [PubMed] [Google Scholar]
- DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
- Donahue J. A. Antistaphylococcal hemolysins and delta hemolysin inhibitor in adult human serum. Can J Microbiol. 1969 Aug;15(8):957–959. doi: 10.1139/m69-167. [DOI] [PubMed] [Google Scholar]
- Fackrell H. B., Wiseman G. M. Immunogenicity of the delta-haemolysin of Staphylococcus aureus. J Med Microbiol. 1974 Nov;7(4):411–414. doi: 10.1099/00222615-7-4-411. [DOI] [PubMed] [Google Scholar]
- Fitton J. E., Dell A., Shaw W. V. The amino acid sequence of the delta haemolysin of Staphylococcus aureus. FEBS Lett. 1980 Jun 30;115(2):209–212. doi: 10.1016/0014-5793(80)81170-7. [DOI] [PubMed] [Google Scholar]
- Forsgren A. Protein A from Staphylococcus aureus. VI. Reaction with subunits from guinea pig gamma-1- and gamma-2-globulin. J Immunol. 1968 May;100(5):927–930. [PubMed] [Google Scholar]
- Gladstone G. P., Yoshida A. The cytopathic action of purified staphylococcal delta-hemolysin. Br J Exp Pathol. 1967 Feb;48(1):11–19. [PMC free article] [PubMed] [Google Scholar]
- Hallander H. O. Characterization and partial purification of staphylococcal delta-lysin. Acta Pathol Microbiol Scand. 1968;72(4):586–600. doi: 10.1111/j.1699-0463.1968.tb00471.x. [DOI] [PubMed] [Google Scholar]
- Heatley N. G. Production of antibody to staphylococcal delta-haemolysin in the rabbit. Br J Exp Pathol. 1977 Aug;58(4):400–411. [PMC free article] [PubMed] [Google Scholar]
- Kantor H. S., Temples B., Shaw W. V. Staphylococcal delta hemolysin: purification and characterization. Arch Biochem Biophys. 1972 Jul;151(1):142–156. doi: 10.1016/0003-9861(72)90483-3. [DOI] [PubMed] [Google Scholar]
- Kapral F. A. Effect of fatty acids on Staphylococcus aureus delta-toxin hemolytic activity. Infect Immun. 1976 Jan;13(1):114–119. doi: 10.1128/iai.13.1.114-119.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapral F. A. Inhibition of Staphylococcus aureus delta hemolysin by phospholipids. Proc Soc Exp Biol Med. 1972 Nov;141(2):519–521. doi: 10.3181/00379727-141-36812. [DOI] [PubMed] [Google Scholar]
- Kapral F. A., Miller M. M. Product of Staphylococcus aureus responsible for the scalded-skin syndrome. Infect Immun. 1971 Nov;4(5):541–545. doi: 10.1128/iai.4.5.541-545.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leslie R. G., Cohen S. Chemical properties of guinea-pig immunoglobulins gamma-1 G, gamma-2 G and gamma M. Biochem J. 1970 Dec;120(4):787–795. doi: 10.1042/bj1200787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniar A. C., Weslake C., Warner P. Prevalence of staphylococcal anti-delta lysin in man. Can J Microbiol. 1967 Aug;13(8):925–929. doi: 10.1139/m67-124. [DOI] [PubMed] [Google Scholar]
- Nolte F. S., Kapral F. A. Binding of radiolabeled Staphylococcus aureus delta-toxin to human erythrocytes. Infect Immun. 1981 Mar;31(3):1086–1093. doi: 10.1128/iai.31.3.1086-1093.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reif A. E. Batch preparation of rabbit gammaG globulin with deae-cellulose. Immunochemistry. 1969 Sep;6(5):723–731. doi: 10.1016/0019-2791(67)90136-x. [DOI] [PubMed] [Google Scholar]
- Turner W. H., Pickard D. J. Neutralisation of immunologically distinct staphylococcal delta haemolysins by antibodies. J Med Microbiol. 1980 Feb;13(1):151–153. doi: 10.1099/00222615-13-1-151. [DOI] [PubMed] [Google Scholar]
- Turner W. H. Purification and characterization of an immunologically distinct delta-hemolysin from a canine strain of Staphylococcus aureus. Infect Immun. 1978 May;20(2):485–494. doi: 10.1128/iai.20.2.485-494.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warr G. W., Hart I. R. Binding of canine IgM and IgG to protein A of Staphylococcus aureus: a simple method for the isolation of canine immunoglobulins from serum and the lymphocyte surface. Am J Vet Res. 1979 Jul;40(7):922–926. [PubMed] [Google Scholar]