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. 1981 Mar;31(3):1251–1260. doi: 10.1128/iai.31.3.1251-1260.1981

Immunogenicity of Staphylococcus aureus delta-toxin.

F S Nolte, F A Kapral
PMCID: PMC351449  PMID: 7014461

Abstract

Studies were conducted to determine the immunogenicity of purified Staphylococcus aureus delta-toxin. Rabbits and guinea pigs immunized with delta-toxin incorporated into a multiple antibody, whereas animals given toxin in saline or toxin in saline with Tween 80 did not produce antibody. The immunoglobulin G (IgG) fraction isolated by chromatography on protein A-Sepharose was examined for the presence of anti-delta-toxin antibody by immunoelectrophoresis, immunodiffusion, quantitative precipitation tests, affinity chromatography, and toxin neutralization tests. Although delta-toxin-specific IgG precipitated the toxin in agar gels, the antibody did not neutralize the toxin's hemolytic activity. Delta-toxin binding to human erythrocyte membranes was demonstrated by indirect immunofluorescent staining of toxin-treated erythrocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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