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. 1981 May;32(2):474–479. doi: 10.1128/iai.32.2.474-479.1981

Ability of an avirulent mutant of Vibrio cholerae to colonize in the infant mouse upper bowel.

S P Sigel, R A Finkelstein, C D Parker
PMCID: PMC351469  PMID: 7251134

Abstract

Vibrio cholerae strain 3083 (biotype El Tor, serotype Ogawa) and Texas Star-SR (SR), a mutant derived from 3083 that produces the B (binding) but not the A (toxic) subunit of choleragen, were compared in their abilities to: (i) associate with the infant mouse upper bowel; (ii) survive and multiple there; and (iii) induce diarrhea. Vibrios labeled with 35SO4 were used to determine association with the upper bowel and ability to multiply. The parental strain associated significantly better than SR, although viable mutant cells were found in the infant mouse intestine 16 to 18 h after challenge. Addition of exogenous toxin enhanced the rate at which labeled SR (but not 3083) was cleared, further suggesting that SR associates less well with the upper bowel. Both SR and 3083 multiplied in the upper bowel but, due perhaps to slight net killing during the first 3 h and its more rapid rate of clearance, SR achieved a population size only 10% that of 3083 by 8 h postchallenge. Strain 3083 elicited diarrhea in infant mice but SR did not, even after 10 successive passages through the infant mouse intestine. Strain SR was slightly temperature sensitive at 37 and 40 degrees C. Its potential use as a live vaccine is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baselski V. S., Medina R. A., Parker C. D. In vivo and in vitro characterization of virulence-deficient mutants of Vibrio cholerae. Infect Immun. 1979 Apr;24(1):111–116. doi: 10.1128/iai.24.1.111-116.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baselski V. S., Medina R. A., Parker C. D. Survival and multiplication of Vibrio cholerae in the upper bowel of infant mice. Infect Immun. 1978 Nov;22(2):435–440. doi: 10.1128/iai.22.2.435-440.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baselski V. S., Parker C. D. Intestinal distribution of Vibrio cholerae in orally infected infant mice: kinetics of recovery of radiolabel and viable cells. Infect Immun. 1978 Aug;21(2):518–525. doi: 10.1128/iai.21.2.518-525.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baselski V. S., Upchurch S., Parker C. D. Isolation and phenotypic characterization of virulence-deficient mutants of Vibrio cholerae. Infect Immun. 1978 Oct;22(1):181–188. doi: 10.1128/iai.22.1.181-188.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baselski V., Briggs R., Parker C. Intestinal fluid accumulation induced by oral challenge with Vibrio cholerae or cholera toxin in infant mice. Infect Immun. 1977 Mar;15(3):704–712. doi: 10.1128/iai.15.3.704-712.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DUTTA N. K., HABBU M. K. Experimental cholera in infant rabbits: a method for chemotherapeutic investigation. Br J Pharmacol Chemother. 1955 Jun;10(2):153–159. doi: 10.1111/j.1476-5381.1955.tb00074.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fernandes P. B., Bayer M. E. Membrane-bound enterotoxin of Vibrio cholerae. J Gen Microbiol. 1977 Dec;103(2):381–387. doi: 10.1099/00221287-103-2-381. [DOI] [PubMed] [Google Scholar]
  8. Holmes R. K., Vasil M. L., Finkelstein R. A. Studies on toxinogenesis in Vibrio cholerae. III. Characterization of nontoxinogenic mutants in vitro and in experimental animals. J Clin Invest. 1975 Mar;55(3):551–560. doi: 10.1172/JCI107962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honda T., Finkelstein R. A. Selection and characteristics of a Vibrio cholerae mutant lacking the A (ADP-ribosylating) portion of the cholera enterotoxin. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2052–2056. doi: 10.1073/pnas.76.4.2052. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nelson E. T., Clements J. D., Finkelstein R. A. Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies. Infect Immun. 1976 Aug;14(2):527–547. doi: 10.1128/iai.14.2.527-547.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schneider D. R., Parker C. D. Isolation and characterization of protease-deficient mutants of vibrio cholerae. J Infect Dis. 1978 Aug;138(2):143–151. doi: 10.1093/infdis/138.2.143. [DOI] [PubMed] [Google Scholar]
  12. Sigel S. P., Lanier S., Baselski V. S., Parker C. D. In vivo evaluation of pathogenicity of clinical and environmental isolates of Vibrio cholerae. Infect Immun. 1980 Jun;28(3):681–687. doi: 10.1128/iai.28.3.681-687.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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