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. 1981 May;32(2):484–489. doi: 10.1128/iai.32.2.484-489.1981

Escherichia coli adhesion to Saccharomyces cerevisiae and mammalian cells: role of piliation and surface hydrophobicity.

K Jann, G Schmidt, E Blumenstock, K Vosbeck
PMCID: PMC351471  PMID: 6114035

Abstract

A number of Escherichia coli strains isolated from patients with urinary tract infections, bacteremia, or diarrhea were studied with respect to their (i) capacity to agglutinate human AB, bovine, and guinea pig erythrocytes as well as yeast (Saccharomyces cerevisiae) cells; (ii) adhesion to monolayers of cells from human intestine (intestine 407; ATCC CCL6), monkey kidney (Vero; ATCC CCL81), feline embryo (Flow no. 05-552), and porcine kidney (PK1; ATCC CRL1392) and of primary rat kidney cell cultures; and (iii) surface hydrophobicity as measured by hydrophobic interaction chromatography. No correlation could be found between the capacity of the bacteria to adhere to the different cultured mammalian cells and their agglutination patterns. The results indicated not only a complexity of bacterial receptors on the eucaryotic cells, but also a multiplicity of bacterial adhesions as expressed by the selectivity of bacterial binding. Binding of bacteria was found to be attributed to the presence of pili on the bacterial surface. It was observed that the bacteria were differently piliated: some had only common type I or related pili which gave rise to mannose-sensitive (MS) adhesion or agglutination (MS pili), some had only pili which gave rise to mannose-resistant (MR) adhesion or agglutination (MR pili), and some had both MS and MR pili. Bacteria with MS pili were more hydrophobic than those with MR pili or with none at all.

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Selected References

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  1. Candy D. C., Chadwick J., Leung T., Phillips A., Harries J. T., Marshall W. C. Adhesion of Enterobacteriaceae to buccal epithelial cells. Lancet. 1978 Nov 25;2(8100):1157–1158. doi: 10.1016/s0140-6736(78)92323-1. [DOI] [PubMed] [Google Scholar]
  2. DUGUID J. P., SMITH I. W., DEMPSTER G., EDMUNDS P. N. Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. J Pathol Bacteriol. 1955 Oct;70(2):335–348. doi: 10.1002/path.1700700210. [DOI] [PubMed] [Google Scholar]
  3. Eden C. S., Eriksson B., Hanson L. A. Adhesion of Escherichia coli to human uroepithelial cells in vitro. Infect Immun. 1977 Dec;18(3):767–774. doi: 10.1128/iai.18.3.767-774.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edén C. S., Hansson H. A. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infect Immun. 1978 Jul;21(1):229–237. doi: 10.1128/iai.21.1.229-237.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans D. G., Evans D. J., Jr, Clegg S., Pauley J. A. Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979 Aug;25(2):738–748. doi: 10.1128/iai.25.2.738-748.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Evans D. G., Evans D. J., Jr New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978 Aug;21(2):638–647. doi: 10.1128/iai.21.2.638-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans D. G., Evans D. J., Jr, Tjoa W. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infect Immun. 1977 Nov;18(2):330–337. doi: 10.1128/iai.18.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans D. G., Silver R. P., Evans D. J., Jr, Chase D. G., Gorbach S. L. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975 Sep;12(3):656–667. doi: 10.1128/iai.12.3.656-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jones G. W., Rutter J. M. Role of the K88 antigen in the pathogenesis of neonatal diarrhea caused by Escherichia coli in piglets. Infect Immun. 1972 Dec;6(6):918–927. doi: 10.1128/iai.6.6.918-927.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Källenius G., Möllby R., Winberg J. In vitro adhesion of uropathogenic Escherichia coli to human periurethral cells. Infect Immun. 1980 Jun;28(3):972–980. doi: 10.1128/iai.28.3.972-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mirelman D., Altmann G., Eshdat Y. Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. J Clin Microbiol. 1980 Apr;11(4):328–331. doi: 10.1128/jcm.11.4.328-331.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ofek I., Beachey E. H. Mannose binding and epithelial cell adherence of Escherichia coli. Infect Immun. 1978 Oct;22(1):247–254. doi: 10.1128/iai.22.1.247-254.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Orskov I., Ferencz A., Orskov F. Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type 1 fimbriated Escherichia coli. Lancet. 1980 Apr 19;1(8173):887–887. doi: 10.1016/s0140-6736(80)91396-3. [DOI] [PubMed] [Google Scholar]
  15. Orskov I., Orskov F., Birch-Andersen A. Comparison of Escherichia coli fimbrial antigen F7 with type 1 fimbriae. Infect Immun. 1980 Feb;27(2):657–666. doi: 10.1128/iai.27.2.657-666.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salit I. E., Gotschlich E. C. Type I Escherichia coli pili: characterization of binding to monkey kidney cells. J Exp Med. 1977 Nov 1;146(5):1182–1194. doi: 10.1084/jem.146.5.1182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schaeffer A. J., Amundsen S. K., Schmidt L. N. Adherence of Escherichia coli to human urinary tract epithelial cells. Infect Immun. 1979 Jun;24(3):753–759. doi: 10.1128/iai.24.3.753-759.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smyth C. J., Jonsson P., Olsson E., Soderlind O., Rosengren J., Hjertén S., Wadström T. Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infect Immun. 1978 Nov;22(2):462–472. doi: 10.1128/iai.22.2.462-472.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sugarman B., Donta S. T. Specificity of attachment of certain enterobacteriaceae to mammalian cells. J Gen Microbiol. 1979 Dec;115(2):509–512. doi: 10.1099/00221287-115-2-509. [DOI] [PubMed] [Google Scholar]
  20. Vosbeck K., Handschin H., Menge E. B., Zak O. Effects of subminimal inhibitory concentrations of antibiotics on adhesiveness of Escherichia coli in vitro. Rev Infect Dis. 1979 Sep-Oct;1(5):845–851. doi: 10.1093/clinids/1.5.845. [DOI] [PubMed] [Google Scholar]
  21. Wevers P., Picken R., Schmidt G., Jann B., Jann K., Golecki J. R., Kist M. Characterization of pili associated with Escherichia coli O18ac. Infect Immun. 1980 Aug;29(2):685–691. doi: 10.1128/iai.29.2.685-691.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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