Figure 4.

RPTP-β regulates HGF-induced HNSCC cell migration. (A) UMSCC-22B metastatic HNSCC tumor cells were infected with empty adenovirus or RPTP-β-expressing adenovirus. (B) UMSCC-22A primary HNSCC tumor cells were infected with NT or RPTP-β. targeting shRNA lentivirus. Forty-eight hours post-infection, confluent cells were treated with 10 µg/ml mitomycin for 30 minutes and scratched with a pipette tip to create a cell-free zone. Cells were then incubated with or without HGF (10 ng/ml) for 48 hours. A representative image of three independent experiments is shown.