Table 1. Primer sequences and PCR conditions for amplification.
Targets | Primer sequences (5′- 3′) | Annealing temperature (°C) |
---|---|---|
Dnmt1 |
CCAAGCTCCGGACCCTGGATGTGT |
64 |
|
CGAGGCCGGTAGTAGTCACAGTAG |
|
Dnmt3a |
GCACCTATGGGCTGCTGCGAAGACG |
64 |
|
CTGCCTCCAATCACCAGGTCGAATG |
|
Dnmt3b |
GTCTGCACACCAGAGACCAGAG |
64 |
|
TCAGAGCCATTCCCATCATCTAC |
|
Bmal1 |
ATGCAGAACACCAAGGAAGG |
64 |
|
CCATCCTTAGCACGGTGAGT |
|
Clock |
CAAAATGTCACGAGCACTTAATGC |
64 |
|
ATATCCACTGCTGGCCTTTGG |
|
Per1 |
ACAGCAGCCACGGTTCTC |
64 |
|
GCTGCCACAGTCCACACA |
|
Per2 |
CAACACAGACGACAGATCA |
64 |
|
TCCTGGTCCTCCTTCAACAC |
|
Cry1 |
CTCGGGTGAGGAGGTTTTCTT |
64 |
|
GACTTCCTCTACCGAGAGCTTCAA |
|
18S rRNA |
TACCACATCCAAGGAAGGCAG |
64 |
TGCCCTCCAATGGATCCTC |