Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 May;32(2):731–738. doi: 10.1128/iai.32.2.731-738.1981

Influence of yeast mannan on human neutrophil functions: inhibition of release of myeloperoxidase related to carbohydrate-binding property of the enzyme.

C D Wright, M J Herron, G R Gray, B Holmes, R D Nelson
PMCID: PMC351508  PMID: 6265362

Abstract

We investigated the possibility that dissolved mannan polysaccharide from Candida may have an inhibitory influence on the host defense mechanisms associated with neutrophils and thereby contribute to the pathogenesis of candidoses. We created a model for this hypothesis by using mannan derived from bakers' yeast (Saccharomyces cerevisiae) and mannan isolated from the serum of a patient with chronic mucocutaneous candidosis. We observed that these mannans inhibited the respiratory burst and release of myeloperoxidase stimulated by phagocytosis of serum-opsonized zymosan in vitro. Mannan did not inhibit release of three other lysosomal enzymes. The selective inhibition of release of myeloperoxidase was attributable to a carbohydrate-binding property of the enzyme, with the mannan causing co-sedimentation of the enzyme with the cellular fraction.

Full text

PDF
731

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodey G. P. Fungal infections complicating acute leukemia. J Chronic Dis. 1966 Jun;19(6):667–687. doi: 10.1016/0021-9681(66)90066-x. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. DeChatelet L. R., Shirley P. S., Johnston R. B., Jr Effect of phorbol myristate acetate on the oxidative metabolism of human polymorphonuclear leukocytes. Blood. 1976 Apr;47(4):545–554. [PubMed] [Google Scholar]
  4. Eickhoff T. C. Infectious complications in renal transplant recipients. Transplant Proc. 1973 Sep;5(3):1233–1238. [PubMed] [Google Scholar]
  5. Ferrante A., Thong Y. H. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique. J Immunol Methods. 1978;24(3-4):389–393. doi: 10.1016/0022-1759(78)90143-6. [DOI] [PubMed] [Google Scholar]
  6. Fiddler M. B., Gray G. R. Immobilization of proteins on aldehyde-activated polyacrylamide supports. Anal Biochem. 1978 Jun 1;86(2):716–724. doi: 10.1016/0003-2697(78)90799-6. [DOI] [PubMed] [Google Scholar]
  7. Fischer A., Ballet J. J., Griscelli C. Specific inhibition of in vitro Candida-induced lymphocyte proliferation by polysaccharidic antigens present in the serum of patients with chronic mucocutaneous candidiasis. J Clin Invest. 1978 Nov;62(5):1005–1013. doi: 10.1172/JCI109204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harrison J. E., Schultz J. Myeloperoxidase: confirmation and nature of heme-binding inequivalence. Resolution of a carbonyl-substituted heme. Biochim Biophys Acta. 1978 Oct 23;536(2):341–349. doi: 10.1016/0005-2795(78)90492-0. [DOI] [PubMed] [Google Scholar]
  9. Hilger A. E., Danley D. L. Alteration of polymorphonuclear leukocyte activity by viable Candida albicans. Infect Immun. 1980 Mar;27(3):714–720. doi: 10.1128/iai.27.3.714-720.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaplan A., Achord D. T., Sly W. S. Phosphohexosyl components of a lysosomal enzyme are recognized by pinocytosis receptors on human fibroblasts. Proc Natl Acad Sci U S A. 1977 May;74(5):2026–2030. doi: 10.1073/pnas.74.5.2026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kerkering T. M., Espinel-Ingroff A., Shadomy S. Detection of candida antigenemia by counterimmunoelectrophoresis in patients with invasive candidiasis. J Infect Dis. 1979 Nov;140(5):659–664. doi: 10.1093/infdis/140.5.659. [DOI] [PubMed] [Google Scholar]
  12. Klebanoff S. J. Myeloperoxidase-halide-hydrogen peroxide antibacterial system. J Bacteriol. 1968 Jun;95(6):2131–2138. doi: 10.1128/jb.95.6.2131-2138.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laforce F. M., Mills D. M., Iverson K., Cousins R., Everett E. D. Inhibition of leukocyte candidacidal activity by serum from patients with disseminated candidiasis. J Lab Clin Med. 1975 Oct;86(4):657–666. [PubMed] [Google Scholar]
  14. Merrill D. P. Purification of human myeloperoxidase by Concanavalin A-Sepharose affinity chromatography. Prep Biochem. 1980;10(2):133–50. doi: 10.1080/00327488008061729. [DOI] [PubMed] [Google Scholar]
  15. Myerowitz R. L., Pazin G. J., Allen C. M. Disseminated candidiasis. Changes in incidence, underlying diseases, and pathology. Am J Clin Pathol. 1977 Jul;68(1):29–38. doi: 10.1093/ajcp/68.1.29. [DOI] [PubMed] [Google Scholar]
  16. Nelson R. D., Herron M. J., Schmidtke J. R., Simmons R. L. Chemiluminescence response of human leukocytes: influence of medium components on light production. Infect Immun. 1977 Sep;17(3):513–520. doi: 10.1128/iai.17.3.513-520.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nelson R. D., Quie P. G., Simmons R. L. Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J Immunol. 1975 Dec;115(6):1650–1656. [PubMed] [Google Scholar]
  18. Sacchi F., Cisternino M. A., Clivio A., Ferrari F. A., Fortunato A., Nespoli L., Beretta A., Siccardi A. G. Disorders of candidacidal activity and other neutrophil functions in three cases of chronic candidiasis. Helv Paediatr Acta. 1979;34(6):607–616. [PubMed] [Google Scholar]
  19. Sando G. N., Neufeld E. F. Recognition and receptor-mediated uptake of a lysosomal enzyme, alpha-l-iduronidase, by cultured human fibroblasts. Cell. 1977 Nov;12(3):619–627. doi: 10.1016/0092-8674(77)90262-8. [DOI] [PubMed] [Google Scholar]
  20. Selvaraj R. J., Zgliczynski J. M., Paul B. B., Sbarra A. J. Enhanced killing of myeloperoxidase-coated bacteria in the myeloperoxidase-H2O2-Cl- system. J Infect Dis. 1978 Apr;137(4):481–485. doi: 10.1093/infdis/137.4.481. [DOI] [PubMed] [Google Scholar]
  21. Spitznagel J. K., Dalldorf F. G., Leffell M. S., Folds J. D., Welsh I. R., Cooney M. H., Martin L. E. Character of azurophil and specific granules purified from human polymorphonuclear leukocytes. Lab Invest. 1974 Jun;30(6):774–785. [PubMed] [Google Scholar]
  22. WARDLAW A. C., PILLEMER L. The properdin system and immunity. V. The bactericidal activity of the properdin system. J Exp Med. 1956 May 1;103(5):553–575. doi: 10.1084/jem.103.5.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weiner M. H., Yount W. J. Mannan antigenemia in the diagnosis of invasive Candida infections. J Clin Invest. 1976 Nov;58(5):1045–1053. doi: 10.1172/JCI108555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. West B. C., Rosenthal A. S., Gelb N. A., Kimball H. R. Separation and characterization of human neutrophil granules. Am J Pathol. 1974 Oct;77(1):41–66. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES