Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 May;32(2):969–972. doi: 10.1128/iai.32.2.969-972.1981

Influence of the Fusogenic Agent Polyethylene Glycol on Attachment of Mycoplasma pneumoniae to Other Cells

Gyan Prakash 1,†, Michael G Gabridge 1,‡
PMCID: PMC351539  PMID: 6788705

Abstract

The effect of fusogenic agents on the interaction of Mycoplasma pneumoniae with human lung fibroblasts was evaluated. A 60-s exposure of the fibroblasts to polyethylene glycol (molecular weight 200; 10%) significantly increased attachment of mycoplasmas to the fibroblasts.

Full text

PDF
969

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anné J., Peberdy J. F. Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions. Arch Microbiol. 1975 Nov 7;105(3):201–205. doi: 10.1007/BF00447138. [DOI] [PubMed] [Google Scholar]
  2. Biberfeld G., Biberfeld P. Ultrastructural features of Mycoplasma pneumoniae. J Bacteriol. 1970 Jun;102(3):855–861. doi: 10.1128/jb.102.3.855-861.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chanock R. M. Mycoplasma infections of man. N Engl J Med. 1965 Dec 2;273(23):1257–concl. doi: 10.1056/NEJM196512022732307. [DOI] [PubMed] [Google Scholar]
  4. Davidson R. L., Gerald P. S. Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic Cell Genet. 1976 Mar;2(2):165–176. doi: 10.1007/BF01542629. [DOI] [PubMed] [Google Scholar]
  5. EDWARDS G. A., FOGH J. Fine structure of pleuropneumonia-like organisms in pure culture and in infected tissue culture cells. J Bacteriol. 1960 Feb;79:267–276. doi: 10.1128/jb.79.2.267-276.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gabridge M. G., Barden-Stahl Y. D., Polisky R. B., Engelhardt J. A. Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium. Infect Immun. 1977 Jun;16(3):766–772. doi: 10.1128/iai.16.3.766-772.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gabridge M. G., Johnson C. K., Cameron A. M. Cytotoxicity of Mycoplasma pneumoniae Membranes. Infect Immun. 1974 Nov;10(5):1127–1134. doi: 10.1128/iai.10.5.1127-1134.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gabridge M. G., Taylor-Robinson D., Davies H. A., Dourmashkin R. R. Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model. Infect Immun. 1979 Jul;25(1):446–454. doi: 10.1128/iai.25.1.446-454.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gabridge M. G., Taylor-Robinson D. Interaction of Mycoplasma pneumoniae with human lung fibroblasts: role of receptor sites. Infect Immun. 1979 Jul;25(1):455–459. doi: 10.1128/iai.25.1.455-459.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grant C. W., McConnell H. M. Fusion of phospholipid vesicles with viable Acholeplasma laidlawii. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1238–1240. doi: 10.1073/pnas.70.4.1238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HUMMELER K., ARMSTRONG D., TOMASSINI N. CYTOPATHOGENIC MYCOPLASMAS ASSOCIATED WITH TWO HUMAN TUMORS. II. MORPHOLOGICAL ASPECTS. J Bacteriol. 1965 Aug;90:511–516. doi: 10.1128/jb.90.2.511-516.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hirachi Y., Kurono M., Kotani S. Polyethylene glycol-induced fusion of L-forms of Staphylococcus aureus. Biken J. 1979 Mar;22(1):25–29. [PubMed] [Google Scholar]
  13. Maisel J. C., Babbitt L. H., John T. J. Fatal Mycoplasma pneumoniae infection with isolation of organisms from lung. JAMA. 1967 Oct 23;202(4):287–290. [PubMed] [Google Scholar]
  14. Manchee R. J., Taylor-Robinson D. Studies on the nature of receptors involved in attachment of tissue culture cells to mycoplasmas. Br J Exp Pathol. 1969 Feb;50(1):66–75. [PMC free article] [PubMed] [Google Scholar]
  15. Organick A. B., Siegesmund K. A., Lutsky I. I. Pneumonia due to mycoplasma in gnotobiotic mice. II. Localization of Mycoplasma pulmonis in the lungs of infected gnotobiotic mice by electron microscopy. J Bacteriol. 1966 Oct;92(4):1164–1176. doi: 10.1128/jb.92.4.1164-1176.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pontecorvo G. Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somatic Cell Genet. 1975 Oct;1(4):397–400. doi: 10.1007/BF01538671. [DOI] [PubMed] [Google Scholar]
  17. Schaeffer P., Cami B., Hotchkiss R. D. Fusion of bacterial protoplasts. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151–2155. doi: 10.1073/pnas.73.6.2151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sobeslavsky O., Prescott B., Chanock R. M. Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J Bacteriol. 1968 Sep;96(3):695–705. doi: 10.1128/jb.96.3.695-705.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Teuber M., Bader J. Action of polymyxin B on bacterial membranes: phosphatidylglycerol- and cardiolipin-induced susceptibility to polymyxin B in Acholeplasma laidlawii B. Antimicrob Agents Chemother. 1976 Jan;9(1):26–35. doi: 10.1128/aac.9.1.26. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wise K. S., Cassell G. H., Action R. T. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4479–4483. doi: 10.1073/pnas.75.9.4479. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES