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. 1981 Jun;32(3):1206–1210. doi: 10.1128/iai.32.3.1206-1210.1981

Measurement of 5′-Nucleotidase in Mouse Peritoneal Macrophages in Listeriosis

C E Pietrangeli 2, E Skamene 1, P J Edelson 3, P A L Kongshavn 1,2
PMCID: PMC351580  PMID: 6265374

Abstract

5′-Nucleotidase activity in peritoneal macrophages of mice was measured both before and after infection with Listeria monocytogenes in hosts which possessed high or low anti-listerial resistance either due to a genetically determined trait or as a result of splenectomy. Reduction in enzyme activity was directly related to the degree of infection that developed in the hosts and hence was inversely related to the level of anti-listerial resistance observed. Basal 5′-nucleotidase activity was significantly lower in noninfected hosts that possessed high anti-listerial resistance, possibly reflecting a higher level of native macrophage activation in these hosts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avruch J., Wallach D. F. Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells. Biochim Biophys Acta. 1971 Apr 13;233(2):334–347. doi: 10.1016/0005-2736(71)90331-2. [DOI] [PubMed] [Google Scholar]
  2. Cheers C., McKenzie I. F., Pavlov H., Waid C., York J. Resistance and susceptibility of mice to bacterial infection: course of listeriosis in resistant or susceptible mice. Infect Immun. 1978 Mar;19(3):763–770. doi: 10.1128/iai.19.3.763-770.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Edelson P. J., Cohn Z. A. 5'-Nucleotidase activity of mouse peritoneal macrophages. I. Synthesis and degradation in resident and inflammatory populations. J Exp Med. 1976 Dec 1;144(6):1581–1595. doi: 10.1084/jem.144.6.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelson P. J., Cohn Z. A. 5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. J Exp Med. 1976 Dec 1;144(6):1596–1608. doi: 10.1084/jem.144.6.1596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Karnovsky M. L., Lazdins J. K. Biochemical criteria for activated macrophages. J Immunol. 1978 Sep;121(3):809–813. [PubMed] [Google Scholar]
  6. Karnovsky M. L., Lazdins J., Drath D., Harper A. Biochemical characteristics of activated macrophages. Ann N Y Acad Sci. 1975 Jun 13;256:266–274. doi: 10.1111/j.1749-6632.1975.tb36053.x. [DOI] [PubMed] [Google Scholar]
  7. MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mackaness G. B. The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969 May 1;129(5):973–992. doi: 10.1084/jem.129.5.973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morahan P. S., Edelson P. J., Gass K. Changes in macrophage ectoenzymes associated with anti-tumor activity. J Immunol. 1980 Sep;125(3):1312–1317. [PubMed] [Google Scholar]
  10. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  11. Skamene E., Chayasirisobhon W. Enhanced resistance to Listeria monocytogenes in splenectomized mice. Immunology. 1977 Dec;33(6):851–858. [PMC free article] [PubMed] [Google Scholar]
  12. Skamene E., Chayasirisobhon W., Konshavn P. A. Increased phagocytic activity of splenectomized mice challenged with Listeria monocytogenes. Immunology. 1978 May;34(5):901–907. [PMC free article] [PubMed] [Google Scholar]
  13. Skamene E., Kongshavn P. A. Phenotypic expression of genetically controlled host resistance to Listeria monocytogenes. Infect Immun. 1979 Jul;25(1):345–351. doi: 10.1128/iai.25.1.345-351.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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