Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Jan;31(1):71–77. doi: 10.1128/iai.31.1.71-77.1981

Direct evidence for the involvement of capsular polysaccharide in the immunoprotective activity of Klebsiella pneumoniae ribosomal preparations.

M M Riottot, J M Fournier, H Jouin
PMCID: PMC351753  PMID: 7012008

Abstract

Previous work has demonstrated the capsular serotypic specificity of the protection conferred on mice by Klebsiella pneumoniae ribosomal preparations. The data in these studies support the hypothesis that capsular polysaccharide plays at least some role in the specificity of the protection conferred by ribosomal preparations. In this investigation, the presence of capsular polysaccharide and lipopolysaccharide in K. pneumoniae ribosomal preparations was demonstrated by using immunodiffusion tests. Lipopolysaccharide content was determined for mice treated with actinomycin D. The serotype of O antigen did not play a role in the orientation of the specificity of the protection. The possibility that lipopolysaccharide might act as an adjuvant was not unlikely since the ribosomal preparations which contained the greatest amounts of lipopolysaccharide appeared to be the most immunoprotective preparations. Ribosomal preparations extracted from a noncapsulated mutant of K. pneumoniae did not protect mice. This finding suggested that capsular polysaccharide might play a role in the immunoprotective activity of ribosomes. This hypothesis was tested by using K. pneumoniae K2 bacteriophage-associated-glycanase, which specifically hydrolyzed K. pneumoniae K2 capsular polysaccharide and thereby suppressed the immunoprotective activity of K. pneumoniae K2 ribosomal preparations. In contrast, the K2 bacteriophage-associated glycanase did not interfere with the immunoprotective activity of K. pneumoniae K1 ribosomal preparations. These results clearly demonstrate that capsular polysaccharide, which is an extraribosomal antigen, is involved in the immunoprotective activity of K. pneumoniae ribosomal preparations.

Full text

PDF

Images in this article

73Image
on p.73

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS M. H., PARK B. H. An enzyme produced by a phage-host cell system. II. The properties of the polysaccharide depolymerase. Virology. 1956 Dec;2(6):719–736. doi: 10.1016/0042-6822(56)90054-x. [DOI] [PubMed] [Google Scholar]
  2. Baba T. Immunogenic activity of a ribosomal fraction obtained from Pasteurella multocida. Infect Immun. 1977 Jan;15(1):1–6. doi: 10.1128/iai.15.1.1-6.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cofré G., Calderón I., Mora G. C. Immunogenic capacity of ribosomes of Salmonella typhi interfered with a flagellin-like material contaminant. Infect Immun. 1978 Apr;20(1):161–166. doi: 10.1128/iai.20.1.161-166.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Corbel M. J. The immunogenic activity of ribosomal fractions derived from Brucella abortus. J Hyg (Lond) 1976 Feb;76(1):65–74. doi: 10.1017/s0022172400054954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DANDEU J. P., QUASH G., BARBU E. PR'ESENCE D'ENDOTOXINE DANS LE PR'EPARATIONS DE RIBOSOMES ET ANTIG'ENICIT'E DES ACIDES NUCL'EIQUES. C R Hebd Seances Acad Sci. 1964 Apr 13;258:3932–3935. [PubMed] [Google Scholar]
  6. Eisenstein T. K., Angerman C. R. Immunity to experimental Salmonella infection: studies on the protective capacity and immunogenicity of lipopolysaccharide, acetone-killed cells, and ribosome-rich extracts of Salmonella typhimurium in C3H/HeJ and CD-1 mice. J Immunol. 1978 Sep;121(3):1010–1014. [PubMed] [Google Scholar]
  7. Eisenstein T. K. Evidence for O antigens as the antigenic determinants in "ribosomal" vaccines prepared from Salmonella. Infect Immun. 1975 Aug;12(2):364–377. doi: 10.1128/iai.12.2.364-377.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gahan L. C., Sandford P. A., Conrad H. E. The structure of the serotype 2 capsular polysaccharide of Aerobacter aerogenes. Biochemistry. 1967 Sep;6(9):2755–2767. doi: 10.1021/bi00861a016. [DOI] [PubMed] [Google Scholar]
  9. Geyer H., Stirm S., Himmelspach K. Immunochemical properties of oligosaccharide-protein conjugates with Klebsiella-K2 specificity. I. Specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies. Med Microbiol Immunol. 1979 Jan 24;165(4):271–288. doi: 10.1007/BF02152925. [DOI] [PubMed] [Google Scholar]
  10. Gonggrijp R., Mullers W. J., Lemmens P. J., van Boven C. P. Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa. Infect Immun. 1980 Jan;27(1):204–210. doi: 10.1128/iai.27.1.204-210.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Guentzel M. N., Berry L. J. Protection of suckling mice from experimental cholera by maternal immunization: comparison of the efficacy of whole-cell, ribosomal-derived, and enterotoxin immunogens. Infect Immun. 1974 Jul;10(1):167–172. doi: 10.1128/iai.10.1.167-172.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoops P., Prather N. E., Berry J., Ravel J. M. Evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium. Infect Immun. 1976 Apr;13(4):1184–1192. doi: 10.1128/iai.13.4.1184-1192.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Levenson V. I., Subbotina Iu L., Gofman I. L., Iarovaia L. M. Ribosomal'naia dizenteriinaia vaktsina. Soobshchenie I. Metod polucheniia, fizicheskie i khimicheskie svoistva. Zh Mikrobiol Epidemiol Immunobiol. 1978 Mar;(3):60–65. [PubMed] [Google Scholar]
  17. Levinson V. I., Subbotina Iu L. Ribosomal'naia dizenteriinaia vaktsina. Soobshchenie II. Biologicheskie ispytaniia v opytakh aktivnoi zashchity morskikh svinok i myshei. Zh Mikrobiol Epidemiol Immunobiol. 1978 Jul;(7):81–86. [PubMed] [Google Scholar]
  18. Lieberman M. M. Direct evidence for the presence of lipopolysaccharide components in Pseudomonas ribosomal vaccine. Infect Immun. 1977 Aug;17(2):471–473. doi: 10.1128/iai.17.2.471-473.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lieberman M. M. Pseudomonas ribosomal vaccines: preparation, properties, and immunogenicity. Infect Immun. 1978 Jul;21(1):76–86. doi: 10.1128/iai.21.1.76-86.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lin J. H., Berry L. J. The use of strain LT2-Ml in identifying the protective antigens in a Salmonella typhimurium-derived ribosomal vaccine. J Reticuloendothel Soc. 1978 Feb;23(2):135–143. [PubMed] [Google Scholar]
  21. Misfeldt M. L., Johnson W. Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium. Infect Immun. 1979 Jun;24(3):808–816. doi: 10.1128/iai.24.3.808-816.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Misfeldt M. L., Johnson W. Protective ability of Salmonella ribosomal protein and RNA in inbred mice. Infect Immun. 1978 Jul;21(1):286–291. doi: 10.1128/iai.21.1.286-291.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Misfeldt M. L., Johnson W. Role of endotoxin contamination in ribiosomal vaccines prepared from Salmonella typhimurium. Infect Immun. 1977 Jul;17(1):98–104. doi: 10.1128/iai.17.1.98-104.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Morris J. A., Orr H. S. Immunity induced in hamsters vaccinated with a ribosome extract of Leptospira interrogans serogroup Icterohaemorrhagiae. J Biol Stand. 1979 Jan;7(1):81–87. doi: 10.1016/s0092-1157(79)80040-2. [DOI] [PubMed] [Google Scholar]
  25. Pieroni R. E., Broderick E. J., Bundeally A., Levine L. A simple method for the quantitation of submicrogram amounts of bacterial endotoxin. Proc Soc Exp Biol Med. 1970 Mar;133(3):790–794. doi: 10.3181/00379727-133-34565. [DOI] [PubMed] [Google Scholar]
  26. Richard C. Etude antigénique et biochimique de 500 souches de Klebsiella. Ann Biol Clin (Paris) 1973;31(4):295–303. [PubMed] [Google Scholar]
  27. Riottot M., Fournier J. M., Pillot J. Capsular serotypic specificity of the protection conferred on mice by Klebsiella pneumoniae ribosomal preparations. Infect Immun. 1979 May;24(2):476–482. doi: 10.1128/iai.24.2.476-482.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  29. Schalla W. O., Johnson W. Immunogenicity of ribosomal vaccines isolated from group A, type 14 Streptococcus pyogenes. Infect Immun. 1975 Jun;11(6):1195–1202. doi: 10.1128/iai.11.6.1195-1202.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Swendsen C. L., Johnson W. Humoral immunity to Streptococcus pneumoniae induced by a pneumococcal ribosomal protein fraction. Infect Immun. 1976 Aug;14(2):345–354. doi: 10.1128/iai.14.2.345-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tewari R. P., Lynn M., Birnbaum A. J., Solotorovsky M. Characterization of the immunoprotective antigen of ribosomal preparations from Haemophilus influenzae. Infect Immun. 1978 Jan;19(1):58–65. doi: 10.1128/iai.19.1.58-65.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tewari R. P., Sharma D., Solotorovsky M., Lafemina R., Balint J. Adoptive transfer of immunity from mice immunized with ribosomes or live yeast cells of Histoplasma capsulatum. Infect Immun. 1977 Mar;15(3):789–795. doi: 10.1128/iai.15.3.789-795.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Thomas D. W., Weiss E. Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infect Immun. 1972 Sep;6(3):355–363. doi: 10.1128/iai.6.3.355-363.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Thompson H. C., Eisenstein T. K. Biological properties of an immunogenic pneumococcal subcellular preparation. Infect Immun. 1976 Mar;13(3):750–757. doi: 10.1128/iai.13.3.750-757.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Winston S., Berry L. J. Immunity induced by ribosomal extracts from Staphylococcus aureus. J Reticuloendothel Soc. 1970 Jul;8(1):66–73. [PubMed] [Google Scholar]
  38. YOUMANS A. S., YOUMANS G. P. NATURE OF THE LABILE IMMUNOGENIC SUBSTANCE IN THE PARTICULATE FRACTION ISOLATED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1964 Oct;88:1030–1037. doi: 10.1128/jb.88.4.1030-1037.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Youmans A. S., Youmans G. P. Immunogenic mycobacterial ribosomal and ribonucleic Acid preparations: chemical and physical characteristics. Infect Immun. 1970 Nov;2(5):659–668. doi: 10.1128/iai.2.5.659-668.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Youmans G. P., Youmans A. S. The effect of mycobacterial RNA on the primary antibody response of mice to bovine globulin. J Immunol. 1972 Aug;109(2):217–221. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES