Abstract
We have previously characterized a rabbit model of invasive Escherichia coli diarrhea. The purpose of this study was to measure the in vitro synthesis of immunoglobulin and anti-invasive E. coli antibody by ileal tissue and levels of anti-invasive E. coli antibody in sera and ileal contents at intervals after diarrhea. Immunoglobulin synthesis by the ileum peaked at 7 to 9 days, but returned to normal by 11 to 13 days post-diarrhea.l Secretory immunoglobulin A (IgA) was synthesized in greater quantity than was IgG or IgM. Anti-invasive E. coli antibody synthesis peaked at 11 to 13 days, decreased to less than half of maximum by 21 to 24 days, but was increased again at 30 to 33 days post-diarrhea. Secretory IgA anti-invasive E. coli antibody was synthesized in greater quantity than was IgG or IgM antibody. Specific antibody of the IgG and IgM classes, but not of the IgA class, appeared in sera by 4 to 6 days and peaked at 7 to 15 days post-diarrhea. Secretory IgA anti-invasive E. coli antibody was detected in ileal contents by 7 to 13 days, but maximum levels were not reached until 50 to 55 days post-diarrhea. IgG and IgM anti-invasive E. coli antibodies were not detected in ileal contents. The synthesis and secretion of secretory IgA antibody were major components of the immune response of the ileum after infection with an invasive bacterium.
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Selected References
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- Cantey J. R., Blake R. K. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis. 1977 Mar;135(3):454–462. doi: 10.1093/infdis/135.3.454. [DOI] [PubMed] [Google Scholar]
- Cantey J. R., O'Hanley P. D., Blake R. K. A rabbit model of diarrhea due to invasive Escherichia coli. J Infect Dis. 1977 Nov;136(5):640–648. doi: 10.1093/infdis/136.5.640. [DOI] [PubMed] [Google Scholar]
- Cantey J. R. Prevention of bacterial infections of mucosal surfaces by immune secretory IgA. Adv Exp Med Biol. 1978;107:461–470. doi: 10.1007/978-1-4684-3369-2_52. [DOI] [PubMed] [Google Scholar]
- Cash R. A., Music S. I., Libonati J. P., Craig J. P., Pierce N. F., Hornick R. B. Response of man to infection with Vibrio cholerae. II. Protection from illness afforded by previous disease and vaccine. J Infect Dis. 1974 Oct;130(4):325–333. doi: 10.1093/infdis/130.4.325. [DOI] [PubMed] [Google Scholar]
- Cebra J. J., Small P. A., Jr Polypeptide chain structure of rabbit immunoglobulins. 3. Secretory gamma-A-immunoglobulin from colostrum. Biochemistry. 1967 Feb;6(2):503–512. doi: 10.1021/bi00854a019. [DOI] [PubMed] [Google Scholar]
- De Buysscher E. V., Dubois P. R. Detection of IgA anti-Escherichia coli plasma cells in the intestine and salivary glands of pigs orally and locally infected with E. coli. Adv Exp Med Biol. 1978;107:593–600. doi: 10.1007/978-1-4684-3369-2_67. [DOI] [PubMed] [Google Scholar]
- Eddie D. S., Schulkind M. L., Robbins J. B. The isolation and biologic activities of purified secretory IgA and IgG anti-Salmonella typhimurium "O" antibodies from rabbit intestinal fluid and colostrum. J Immunol. 1971 Jan;106(1):181–190. [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Fubara E. S., Freter R. Protection against enteric bacterial infection by secretory IgA antibodies. J Immunol. 1973 Aug;111(2):395–403. [PubMed] [Google Scholar]
- Ganguly R., Clem L. W., Bencić Z., Sinha R., Sakazaki R., Waldman R. H. Antibody response in the intestinal secretions of volunteers immunized with various cholera vaccines. Bull World Health Organ. 1975;52(3):323–330. [PMC free article] [PubMed] [Google Scholar]
- Hand W. L., Cantey J. R. Antibacterial mechanisms of the lower respiratory tract. I. Immunoglobulin synthesis and secretion. J Clin Invest. 1974 Feb;53(2):354–362. doi: 10.1172/JCI107567. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hohmann A., Schmidt G., Rowley D. Intestinal and serum antibody responses in mice after oral immunization with Salmonella, Escherichia coli, and Salmonella-Escherichia coli hybrid strains. Infect Immun. 1979 Jul;25(1):27–33. doi: 10.1128/iai.25.1.27-33.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmgren J., Svennerholm A. M., Ouchterlony O., Anderson A., Walletström G., Westerberg-Berndtsson U. Antitoxic immunity in experimental cholera: protection, and serum and local antibody responses in rabbits after enteral and parenteral immunization. Infect Immun. 1975 Dec;12(6):1331–1340. doi: 10.1128/iai.12.6.1331-1340.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Husband A. J., Gowans J. L. The origin and antigen-dependent distribution of IgA-containing cells in the intestine. J Exp Med. 1978 Nov 1;148(5):1146–1160. doi: 10.1084/jem.148.5.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson G. D., Lemaître-Coelho I., Vaerman J. P., Bazin H., Beckers A. Rapid disappearance from serum of intravenously injected rat myeloma IgA and its secretion into bile. Eur J Immunol. 1978 Feb;8(2):123–126. doi: 10.1002/eji.1830080210. [DOI] [PubMed] [Google Scholar]
- Kaur J., Burrows W., Furlong M. A. Immunity to cholera: antibody response in the lower ileum of the rabbit. J Infect Dis. 1971 Oct;124(4):359–366. doi: 10.1093/infdis/124.4.359. [DOI] [PubMed] [Google Scholar]
- Keren D. F., Holt P. S., Collins H. H., Gemski P., Formal S. B. The role of Peyer's patches in the local immune response of rabbit ileum to live bacteria. J Immunol. 1978 Jun;120(6):1892–1896. [PubMed] [Google Scholar]
- Lange S., Hansson H. A., Molin S. O., Nygren H. Local cholera immunity in mice: intestinal antitoxin-containing cells and their correlation with protective immunity. Infect Immun. 1979 Mar;23(3):743–750. doi: 10.1128/iai.23.3.743-750.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lehmann J. D., Smith J. W., Miller T. E., Barnett J. A., Sanford J. P. Local immune response in experimental pyelonephritis. J Clin Invest. 1969 Nov;47(11):2541–2550. doi: 10.1172/JCI105936. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine M. M., Nalin D. R., Hoover D. L., Bergquist E. J., Hornick R. B., Young C. R. Immunity to enterotoxigenic Escherichia coli. Infect Immun. 1979 Mar;23(3):729–736. doi: 10.1128/iai.23.3.729-736.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Menzel J., Rowley D. Immunoglobulin synthesis and antibody content in the small intestine of the rabbit. Clin Exp Immunol. 1975 Mar;19(3):551–562. [PMC free article] [PubMed] [Google Scholar]
- Merrill D., Hartley T. F., Claman H. N. Electroimmunodiffusion (EID): a simple, rapid method for quantitation of immunoglobulins in dilute biological fluids. J Lab Clin Med. 1967 Jan;69(1):151–159. [PubMed] [Google Scholar]
- O'Daly J. A., Cebra J. J. Rabbit secretory IgA. I. Isolation of secretory component after selective dissociation of the immunoglobulin. J Immunol. 1971 Aug;107(2):436–448. [PubMed] [Google Scholar]
- O'Hanley P. D., Cantey J. R. Surface structures of Escherichia coli that produce diarrhea by a variety of enteropathic mechanisms. Infect Immun. 1978 Sep;21(3):874–878. doi: 10.1128/iai.21.3.874-878.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orlans E., Peppard J., Fry J. F., Hinton R. H., Mullock B. M. Secretory component as the receptor for polymeric IgA on rat hepatocytes. J Exp Med. 1979 Dec 1;150(6):1577–1581. doi: 10.1084/jem.150.6.1577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce N. F., Cray W. C., Jr, Sircar B. K. Induction of a mucosal antitoxin response and its role in immunity to experimental canine cholera. Infect Immun. 1978 Jul;21(1):185–193. doi: 10.1128/iai.21.1.185-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce N. F., Gowans J. L. Cellular kinetics of the intestinal immune response to cholera toxoid in rats. J Exp Med. 1975 Dec 1;142(6):1550–1563. doi: 10.1084/jem.142.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter P., Kenworthy R., Noakes D. E., Allen W. D. Intestinal antibody secretion in the young pig in response to oral immunization with Escherichia coli. Immunology. 1974 Nov;27(5):841–853. [PMC free article] [PubMed] [Google Scholar]
- Rowley D. Specific immune antibacterial mechanisms in the intestines of mice. Am J Clin Nutr. 1974 Dec;27(12):1417–1423. doi: 10.1093/ajcn/27.12.1417. [DOI] [PubMed] [Google Scholar]
- Smiley J. D., Sachs C., Ziff M. In vitro synthesis of immunoglobulin by rheumatoid synovial membrane. J Clin Invest. 1968 Mar;47(3):624–632. doi: 10.1172/JCI105758. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith J. W., Kaijser B. The local immune response to Escherichia coli O and K antigen in experimental pyelonephritis. J Clin Invest. 1976 Aug;58(2):276–281. doi: 10.1172/JCI108469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steele E. J., Chaicumpa W., Rowley D. Isolation and biological properties of three classes of rabbit antibody to Vibrio cholerae. J Infect Dis. 1974 Aug;130(2):93–103. doi: 10.1093/infdis/130.2.93. [DOI] [PubMed] [Google Scholar]
- Svennerholm A. M., Holmgren J. Immunoglobulin and specific-antibody synthesis in vitro by enteral and nonenteral lymphoid tissues after subcutaneous cholera immunization. Infect Immun. 1977 Feb;15(2):360–369. doi: 10.1128/iai.15.2.360-369.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Svennerholm A., Lange S., Holmgren J. Correlation between intestinal synthesis of specific immunoglobulin A and protection against experimental cholera in mice. Infect Immun. 1978 Jul;21(1):1–6. doi: 10.1128/iai.21.1.1-6.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yardley J. H., Keren D. F., Hamilton S. R., Brown G. D. Local (immunoglobulin A) immune response by the intestine to cholera toxin and its partial suppression with combined systemic and intra-intestinal immunization. Infect Immun. 1978 Feb;19(2):589–597. doi: 10.1128/iai.19.2.589-597.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]