Abstract
The elaboration of extracellular proteolytic activity by Candida albicans during growth in laboratory broth or in human whole salivary supernatant was investigated. Growth of the organism in broth at pH 3 to 7 followed by assay of culture supernatants at pH 4 (optimum for activity) indicated protease was only present in cultures grown at a pH of less than 5. In contrast, growth in sterile human whole salivary supernatant over the pH range of 3 to 7 uniformly failed to result in production of protease. Growth of the organism at pH 4 in broth supplemented with saliva resulted in a saliva-dependent inhibition of protease production. Although the addition of up to 16% (vol/vol) saliva had essentially no effect on growth, 4% saliva caused a 50% reduction in proteolysis of substrate protein. Due to the low pH requirement for protease production and activity and the demonstration that saliva is a potent inhibitor of protease synthesis, we conclude C. albicans most likely does not produce extracellular protease in the human oral cavity.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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