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. 1981 Jan;31(1):386–390. doi: 10.1128/iai.31.1.386-390.1981

Membrane and cytoplasmic location of streptolysin S precursor.

G B Calandra, R M Cole
PMCID: PMC351795  PMID: 7011998

Abstract

Group A streptococci which produce streptolysin S (SLS) contain a cellular potential hemolysin (CPH) which is precursor to extracellular SLS. Since the cellular location of CPH is unknown, protoplasts prepared with group C phage-associated lysin or mutanolysin from 18 strains of group A streptococci were fractionated into subcellular components and assayed for CPH. In all strains, most of the CPH was membrane associated, and most could not be removed from membranes by washing with buffer or 2 M LiCl. CPH remaining in the cytoplasmic fraction was sedimentable, but not associated with membrane fragments. Ribonuclease digestion neither solubilized nor inactivated CPH from membranes. Streptococcal proteinase also did not affect CPH, although it did inactivate SLS. We conclude that group A streptococci contain a major pool of CPH in the membrane and a smaller pool in the cytoplasm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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