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. 2012 Oct 2;3(5):e00303-12. doi: 10.1128/mBio.00303-12

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Copyright © 2012 Bek-Thomsen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 7 — ZmpC protease activity assay. Zymogram gel showing gelatin cleavage by MMP-9 (matrix metalloprotease 9) and the proteolytic effect of ZmpC. MMP-9 was preincubated with different bacteria prior to electrophoresis. Lane 1, control (no bacteria); lane 2, S. mitis SK597 (negative for ZmpC); lane 3, S. mitis SK142 (positive for ZmpC); lane 4, S. pneumoniae 276/95 (positive for ZmpC); lane 5, S. pneumoniae TIGR4 ΔzmpC mutant; lane 6, S. pneumoniae TIGR4 ΔzmpB mutant; lane 7, S. pneumoniae TIGR4 Δiga mutant; lane 8, S. pneumoniae TIGR4 (positive for ZmpC); lane 9, S. pneumoniae R6 (negative for ZmpC).