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. 1977 Jan;11(1):31–33. doi: 10.1128/aac.11.1.31

Bioassay Method for Polyene Antibiotics Based on the Measurement of Rubidium Efflux from Rubidium-Loaded Yeast Cells

R F Cosgrove 1, J E Fairbrother 1
PMCID: PMC351913  PMID: 319752

Abstract

A bioassay method for the polyene antibiotics nystatin and amphotericin B is proposed based on the measurement of the efflux of rubidium ions from a rubidium-loaded yeast culture challenged with the antibiotics. For this purpose a major proportion of the intracellular K+ ions in a Saccharomyces cerevisiae culture has been substituted by Rb+ ions. The rubidium leakage is measured by atomic absorption spectrophotometry, and a straight-line, dose-response correlation has been obtained for both antibiotics.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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