Abstract
A rapid method for determining the minimal inhibition concentrations (MICs) of ampicillin using Haemophilus influenzae as the test organism is described. This semiautomated technique using the Autobac system was compared with the agar dilution MIC method. Fifty-seven isolates of both susceptible and resistant H. influenzae were tested. There was exact quantitative agreement with 65% of the isolates, and 91% of the isolates showed no more than a one-dilution-interval difference when MICs were determined by both methods. Total testing time was 3 to 4 h.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ericsson H. M., Sherris J. C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;217(Suppl):1+–1+. [PubMed] [Google Scholar]
- Farrar W. E., Jr, O'Dell N. M. Beta-lactamase activity in ampicillin-resistant Haemophilus influenzae. Antimicrob Agents Chemother. 1974 Nov;6(5):625–629. doi: 10.1128/aac.6.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorgensen J. H., Jones P. M. Simplified medium for ampicillin susceptibility testing of Haemophilus influenzae. Antimicrob Agents Chemother. 1975 Feb;7(2):186–190. doi: 10.1128/aac.7.2.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khan W., Ross S., Rodriguez W., Controni G., Saz A. K. Haemophilus influenzae type B resistant to ampicillin. A report of two cases. JAMA. 1974 Jul 15;229(3):298–301. [PubMed] [Google Scholar]
- Manten A., van Klingeren B., Dessens-Kroon M. Chloramphenicol resistance in Haemophilus influenzae. Lancet. 1976 Mar 27;1(7961):702–702. doi: 10.1016/s0140-6736(76)92832-4. [DOI] [PubMed] [Google Scholar]
- Mathies A. W., Jr Penicillins in the treatment of bacterial meningitis. J R Coll Physicians Lond. 1972 Jan;6(2):139–146. [PMC free article] [PubMed] [Google Scholar]
- Rubin F. A., Smith D. H. Characterization of R factor beta-lactamases by the acidimetric method. Antimicrob Agents Chemother. 1973 Jan;3(1):68–73. doi: 10.1128/aac.3.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith A. L. Antibiotics and invasive Haemophilus influenzae. N Engl J Med. 1976 Jun 10;294(24):1329–1331. doi: 10.1056/NEJM197606102942406. [DOI] [PubMed] [Google Scholar]
- Thomas W. J., McReynolds J. W., Mock C. R., Bailey D. W. Letter: Ampicillin-resistant Haemophilus influenzae meningitis. Lancet. 1974 Feb 23;1(7852):313–313. doi: 10.1016/s0140-6736(74)92617-8. [DOI] [PubMed] [Google Scholar]
- Thornsberry C., Kirven L. A. Ampicillin resistance in Haemophilus influenzae as determined by a rapid test for beta-lactamase production. Antimicrob Agents Chemother. 1974 Nov;6(5):653–654. doi: 10.1128/aac.6.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]