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. 1977 Mar;11(3):541–552. doi: 10.1128/aac.11.3.541

Comparison of the Effects of Mecillinam and 6-Aminopenicillanic Acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus

Victor Lorian 1, Barbara Atkinson 2
PMCID: PMC352018  PMID: 193439

Abstract

Single strains of Proteus mirabilis, Escherichia coli, and Staphylococcus aureus were grown on filter membranes placed on agar containing concentration series of mecillinam (FL 1060), 6-aminopenicillanic acid (6-APA), or ampicillin. P. mirabilis and E. coli were also exposed to combinations of mecillinam or 6-APA with ampicillin. Colony-forming units were counted, and cells were examined by interference phase-contrast and transmission electron microscopy. Mecillinam and 6-APA were very effective in reducing the viability of the two gram-negative species, but they were less effective against S. aureus. Combinations of mecillinam and 6-APA with ampicillin acted synergistically against the gram-negative bacilli. When the antibiotics were presented consecutively, their effects on viability were usually no greater than the effects of the individual antibiotics acting alone. When P. mirabilis and E. coli were exposed to mecillinam alone or in combination with ampicillin, the cells became rounded. 6-APA alone or in combination with ampicillin produced elongated polymorphic cells in these species. The most unusual morphological effects were ultrastructural. Mecillinam and, to a lesser extent, 6-APA produced inward growth of numerous pairs of trilamellar membranous structures within the cells. It is possible that these membranes represent the growth initiation of aberrant cross walls. Both mecillinam and 6-APA produced multiple, thick cross walls in S. aureus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore R. S., Klein J. O., Wilcox C., Finland M. Synergy of mecillinam (FL1060) with penicillins and cephalosporins against Proteus and Klebsiella, with observations on combinations with other antibiotics and against other bacterial species. Antimicrob Agents Chemother. 1976 Apr;9(4):701–705. doi: 10.1128/aac.9.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FLEMING A., VOUREKA A., KRAMER I. R. H., HUGHES W. H. The morphology and motility of Proteus vulgaris and other organisms cultured in the presence of penicillin. J Gen Microbiol. 1950 May;4(2):257–269. doi: 10.1099/00221287-4-2-257. [DOI] [PubMed] [Google Scholar]
  3. Gilleland H. E., Jr, Murray R. G. Ultrastructural study of polymyxin-resistant isolates of Pseudomonas aeruginosa. J Bacteriol. 1976 Jan;125(1):267–281. doi: 10.1128/jb.125.1.267-281.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greenwood D., O'Grady F. FL 1060: a new beta-lactam antibiotic with novel properties. J Clin Pathol. 1973 Jan;26(1):1–6. doi: 10.1136/jcp.26.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grunberg E., Cleeland R., Beskid G., DeLorenzo W. F. In vivo synergy between 6 beta-amidinopenicillanic acid derivatives and other antibiotics. Antimicrob Agents Chemother. 1976 Apr;9(4):589–594. doi: 10.1128/aac.9.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lorain V., Sabath L. D. Penicillins and cephalosporins: differences in morphologic effects on Proteus mirabilis. J Infect Dis. 1972 May;125(5):560–564. doi: 10.1093/infdis/125.5.560. [DOI] [PubMed] [Google Scholar]
  7. Lorian V., Atkinson B. Effects of subinhibitory concentrations of antibiotics on cross walls of cocci. Antimicrob Agents Chemother. 1976 Jun;9(6):1043–1055. doi: 10.1128/aac.9.6.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lorian V., Popoola B. The effect of nitrofurantoin on the morphology of gram-negative bacilli. J Infect Dis. 1972 Feb;125(2):187–189. doi: 10.1093/infdis/125.2.187. [DOI] [PubMed] [Google Scholar]
  9. Lorian V., Sabath L. D., Simionescu M., Watson D. W. Decrease in ribosomal density of Proteus mirabilis exposed to subinhibitory concentrations of ampicillin or cephalothin. Proc Soc Exp Biol Med. 1975 Jul;149(3):731–735. doi: 10.3181/00379727-149-38888. [DOI] [PubMed] [Google Scholar]
  10. Lorian V. Some effect of subinbilitory concentrations of penicillin on the structure and division of staphylococci. Antimicrob Agents Chemother. 1975 Jun;7(6):864–867. doi: 10.1128/aac.7.6.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lorian V. Some effects of subinhibitory concentrations of antibiotics on bacteria. Bull N Y Acad Med. 1975 Oct;51(9):1046–1055. [PMC free article] [PubMed] [Google Scholar]
  12. Matsuhashi S., Kamiryo T., Blumberg P. M., Linnett P., Willoughby E., Strominger J. L. Mechanism of action and development of resistance to a new amidino penicillin. J Bacteriol. 1974 Feb;117(2):578–587. doi: 10.1128/jb.117.2.578-587.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Melchior N. H., Blom J., Tybring L., Birch-Andersen A. Light and electron microscopy of the early response of Escherichia coli to a 6beta-amidinopenicillanic acid (FL 1060). Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Aug;81(4):393–407. doi: 10.1111/j.1699-0463.1973.tb02222.x. [DOI] [PubMed] [Google Scholar]
  14. Neu H. C. Mecillinam, a novel penicillanic acid derivative with unusual activity against gram-negative bacteria. Antimicrob Agents Chemother. 1976 May;9(5):793–799. doi: 10.1128/aac.9.5.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Spratt B. G. Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K12. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2999–3003. doi: 10.1073/pnas.72.8.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spratt B. G., Pardee A. B. Penicillin-binding proteins and cell shape in E. coli. Nature. 1975 Apr 10;254(5500):516–517. doi: 10.1038/254516a0. [DOI] [PubMed] [Google Scholar]
  17. Tybring L., Melchior N. H. Mecillinam (FL 1060), a 6beta-amidinopenicillanic acid derivative: bactericidal action and synergy in vitro. Antimicrob Agents Chemother. 1975 Sep;8(3):271–276. doi: 10.1128/aac.8.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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