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. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: Yeast. 2012 Nov 21;29(12):519–530. doi: 10.1002/yea.2932

Comparison of pheromone induction profiles for FUS1 and reporter gene expression. (A) Plot comparing pheromone induction profiles for FUS1 () and GFP* () mRNA in ^PFUS1-UBI-MΔkGFP* (C699-198) and ^PFUS1-UBI-YΔkGFP* (C699-199) strains. Data points are the average from six independent induction time courses (three with strain C699-198 and three with strain C699-199). Error bars show 95% confidence limits. (B) Plot comparing the pheromone induction profile for GFP* fluorescence in the ^PFUS1-UBI-MΔkGFP* (C699-198) () and ^PFUS1-UBI-YΔkGFP* (C699-199) reporter strains. More than 25 or 50 individual cells were scored for each time point in a single time course with strain C699-198 or C699-199, respectively. Each data point is the average from four independent time courses. Error bars show 95% confidence limits. (C) Model prediction of the pheromone induced FUS1 mRNA profile using YΔkGFP* fluorescence measurements as input. Plot compares the model mean () and range (min, max ) to the empirically determined reporter GFP* mRNA profile from (A).

Inline graphic — Comparison of pheromone induction profiles for FUS1 and reporter gene expression. (A) Plot comparing pheromone induction profiles for FUS1 () and GFP* () mRNA in ^PFUS1-UBI-MΔkGFP* (C699-198) and ^PFUS1-UBI-YΔkGFP* (C699-199) strains. Data points are the average from six independent induction time courses (three with strain C699-198 and three with strain C699-199). Error bars show 95% confidence limits. (B) Plot comparing the pheromone induction profile for GFP* fluorescence in the ^PFUS1-UBI-MΔkGFP* (C699-198) () and ^PFUS1-UBI-YΔkGFP* (C699-199) reporter strains. More than 25 or 50 individual cells were scored for each time point in a single time course with strain C699-198 or C699-199, respectively. Each data point is the average from four independent time courses. Error bars show 95% confidence limits. (C) Model prediction of the pheromone induced FUS1 mRNA profile using YΔkGFP* fluorescence measurements as input. Plot compares the model mean () and range (min, max ) to the empirically determined reporter GFP* mRNA profile from (A).