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. 1977 Jun;11(6):993–998. doi: 10.1128/aac.11.6.993

Evaluation of Three Newer Methods for Investigating Protein Interactions of Penicillin G

Lancer R Peterson 1, Dale N Gerding 1, Horace H Zinneman 1, Barbara M Moore 1
PMCID: PMC352116  PMID: 879764

Abstract

The interaction of penicillin G with human serum proteins was evaluated by three different techniques: rate of dialysis, cross-linked dextran exclusion, and ultracentrifugation. The rate-of-dialysis technique demonstrated that penicillin G binding to serum was immediate but incompletely reversible. Cross-linked dextran adsorbed or trapped significant amounts of penicillin G, necessitating correction factors of more than 10%. Ultracentrifugation was found to be the most reliable method for quantitative protein-binding determinations of penicillins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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